No Cover Image

E-Thesis 291 views 733 downloads

Exploring metabolomic biomarkers using mass spectrometry techniques / KATHRYN COATES

Swansea University Author: KATHRYN COATES

  • Coates_Kathryn_T_PhD_Thesis_Final_Cronfa.pdf

    PDF | E-Thesis – open access

    Copyright: The Author, Kathryn T. Coates, 2025.

    Download (6.43MB)

DOI (Published version): 10.23889/SUthesis.69791

Abstract

Mass spectrometry based metabolomic approaches prove an excellent source for biomarker discovery through their ability to seek out robust and unique disease signatures in a fast, accurate and high throughput manner whilst delivering a great deal of sensitivity and biological detail from a broad rang...

Full description

Published: Swansea, Wales, UK 2025
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: Dudley, Ed ; Thornton, Catherine
URI: https://cronfa.swan.ac.uk/Record/cronfa69791
first_indexed 2025-06-23T10:32:21Z
last_indexed 2025-06-24T04:56:12Z
id cronfa69791
recordtype RisThesis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2025-06-23T11:42:10.1336244</datestamp><bib-version>v2</bib-version><id>69791</id><entry>2025-06-23</entry><title>Exploring metabolomic biomarkers using mass spectrometry techniques</title><swanseaauthors><author><sid>b32c35cc413d8cbe1abc9fcae4f35d4f</sid><firstname>KATHRYN</firstname><surname>COATES</surname><name>KATHRYN COATES</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2025-06-23</date><abstract>Mass spectrometry based metabolomic approaches prove an excellent source for biomarker discovery through their ability to seek out robust and unique disease signatures in a fast, accurate and high throughput manner whilst delivering a great deal of sensitivity and biological detail from a broad range of sample source. This study has identified a number of unique and novel biological signatures to improve diagnosis/ metabolic pathway understanding for a broad range of conditions. Chapter 2- Pancreatic cancer is a disease that occludes diagnosis through its lack of unique symptomatic presentation in patients leading to late-stage diagnosis, and low survival rates. This chapter successfully identifies novel metabolites capable of distinguishing pancreatic cancer from healthy and pancreatitis conditions using both internally held metabolites and externally excreted modified nucleosides for both diagnosis and screening potential. Further experimental analysis using SILAC to trace glucose and glutamine use by cancerous and healthy cells has been used to elucidate differences in energy resource utilisation. Chapter 3- Looks at the development of a metabolomic analysis capable of distinguishing various serum-based conditions from each other as well as analysing metabolic profile changes between males, females (pregnant and non-pregnant) and cord samples to establish key profile changes that occur in pregnancy as well as establishing how these changes may be conveyed in breast-milk production to further support baby development after birth. Chapter 4- Captures faecal markers capable of distinguishing successfully from unsuccessfully reproducing captive elephant, seeking diagnostic and explanatory, indicating potential upstream mis-regulations that could be causative of the ailment, markers aiding to potential treatments or future preventions to aid reproduction. Chapter 5- Evaluates drosophila fly as a suitable model for future neurological studies including Alzheimer&#x2019;s disease. This study has successfully identified numerous lipids, including plasmalogens, in fly heads that make them a suitable candidate in future studies as well as showing the lipidomic changes resultant of disrupted metabolic pathways associated with Alzheimer&#x2019;s disease. Chapter 6- Establishes the relationship between taurine consumption and exercise performance relating to thermoregulation, through detecting and analysing taurine&#x2019;s presence in plasma samples taken before and after exercise periods.</abstract><type>E-Thesis</type><journal/><volume/><journalNumber/><paginationStart/><paginationEnd/><publisher/><placeOfPublication>Swansea, Wales, UK</placeOfPublication><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic/><keywords>Mass spectrometry, GC-MS, MALDI, Shotgun lipidomics, Orbitrap, HPLC, UPLC, biomarkers, metabolomics, cell culture, heavy metabolite tracing, fertility, cancer, serum, plasma, urine, faeces, drosophila, Alzheimer&#x2019;s, plasmalogens, Taurine, Lipids, amino acids, modified nucleosides, thermoregulation</keywords><publishedDay>10</publishedDay><publishedMonth>6</publishedMonth><publishedYear>2025</publishedYear><publishedDate>2025-06-10</publishedDate><doi>10.23889/SUthesis.69791</doi><url/><notes/><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><supervisor>Dudley, Ed ; Thornton, Catherine</supervisor><degreelevel>Doctoral</degreelevel><degreename>Ph.D</degreename><apcterm/><funders/><projectreference/><lastEdited>2025-06-23T11:42:10.1336244</lastEdited><Created>2025-06-23T11:22:11.0825385</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Biomedical Science</level></path><authors><author><firstname>KATHRYN</firstname><surname>COATES</surname><order>1</order></author></authors><documents><document><filename>69791__34540__47119b7e3bc74b34a09e3e200059ffa1.pdf</filename><originalFilename>Coates_Kathryn_T_PhD_Thesis_Final_Cronfa.pdf</originalFilename><uploaded>2025-06-23T11:39:23.3204842</uploaded><type>Output</type><contentLength>6747403</contentLength><contentType>application/pdf</contentType><version>E-Thesis &#x2013; open access</version><cronfaStatus>true</cronfaStatus><documentNotes>Copyright: The Author, Kathryn T. Coates, 2025.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2025-06-23T11:42:10.1336244 v2 69791 2025-06-23 Exploring metabolomic biomarkers using mass spectrometry techniques b32c35cc413d8cbe1abc9fcae4f35d4f KATHRYN COATES KATHRYN COATES true false 2025-06-23 Mass spectrometry based metabolomic approaches prove an excellent source for biomarker discovery through their ability to seek out robust and unique disease signatures in a fast, accurate and high throughput manner whilst delivering a great deal of sensitivity and biological detail from a broad range of sample source. This study has identified a number of unique and novel biological signatures to improve diagnosis/ metabolic pathway understanding for a broad range of conditions. Chapter 2- Pancreatic cancer is a disease that occludes diagnosis through its lack of unique symptomatic presentation in patients leading to late-stage diagnosis, and low survival rates. This chapter successfully identifies novel metabolites capable of distinguishing pancreatic cancer from healthy and pancreatitis conditions using both internally held metabolites and externally excreted modified nucleosides for both diagnosis and screening potential. Further experimental analysis using SILAC to trace glucose and glutamine use by cancerous and healthy cells has been used to elucidate differences in energy resource utilisation. Chapter 3- Looks at the development of a metabolomic analysis capable of distinguishing various serum-based conditions from each other as well as analysing metabolic profile changes between males, females (pregnant and non-pregnant) and cord samples to establish key profile changes that occur in pregnancy as well as establishing how these changes may be conveyed in breast-milk production to further support baby development after birth. Chapter 4- Captures faecal markers capable of distinguishing successfully from unsuccessfully reproducing captive elephant, seeking diagnostic and explanatory, indicating potential upstream mis-regulations that could be causative of the ailment, markers aiding to potential treatments or future preventions to aid reproduction. Chapter 5- Evaluates drosophila fly as a suitable model for future neurological studies including Alzheimer’s disease. This study has successfully identified numerous lipids, including plasmalogens, in fly heads that make them a suitable candidate in future studies as well as showing the lipidomic changes resultant of disrupted metabolic pathways associated with Alzheimer’s disease. Chapter 6- Establishes the relationship between taurine consumption and exercise performance relating to thermoregulation, through detecting and analysing taurine’s presence in plasma samples taken before and after exercise periods. E-Thesis Swansea, Wales, UK Mass spectrometry, GC-MS, MALDI, Shotgun lipidomics, Orbitrap, HPLC, UPLC, biomarkers, metabolomics, cell culture, heavy metabolite tracing, fertility, cancer, serum, plasma, urine, faeces, drosophila, Alzheimer’s, plasmalogens, Taurine, Lipids, amino acids, modified nucleosides, thermoregulation 10 6 2025 2025-06-10 10.23889/SUthesis.69791 COLLEGE NANME COLLEGE CODE Swansea University Dudley, Ed ; Thornton, Catherine Doctoral Ph.D 2025-06-23T11:42:10.1336244 2025-06-23T11:22:11.0825385 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science KATHRYN COATES 1 69791__34540__47119b7e3bc74b34a09e3e200059ffa1.pdf Coates_Kathryn_T_PhD_Thesis_Final_Cronfa.pdf 2025-06-23T11:39:23.3204842 Output 6747403 application/pdf E-Thesis – open access true Copyright: The Author, Kathryn T. Coates, 2025. true eng
title Exploring metabolomic biomarkers using mass spectrometry techniques
spellingShingle Exploring metabolomic biomarkers using mass spectrometry techniques
KATHRYN COATES
title_short Exploring metabolomic biomarkers using mass spectrometry techniques
title_full Exploring metabolomic biomarkers using mass spectrometry techniques
title_fullStr Exploring metabolomic biomarkers using mass spectrometry techniques
title_full_unstemmed Exploring metabolomic biomarkers using mass spectrometry techniques
title_sort Exploring metabolomic biomarkers using mass spectrometry techniques
author_id_str_mv b32c35cc413d8cbe1abc9fcae4f35d4f
author_id_fullname_str_mv b32c35cc413d8cbe1abc9fcae4f35d4f_***_KATHRYN COATES
author KATHRYN COATES
author2 KATHRYN COATES
format E-Thesis
publishDate 2025
institution Swansea University
doi_str_mv 10.23889/SUthesis.69791
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description Mass spectrometry based metabolomic approaches prove an excellent source for biomarker discovery through their ability to seek out robust and unique disease signatures in a fast, accurate and high throughput manner whilst delivering a great deal of sensitivity and biological detail from a broad range of sample source. This study has identified a number of unique and novel biological signatures to improve diagnosis/ metabolic pathway understanding for a broad range of conditions. Chapter 2- Pancreatic cancer is a disease that occludes diagnosis through its lack of unique symptomatic presentation in patients leading to late-stage diagnosis, and low survival rates. This chapter successfully identifies novel metabolites capable of distinguishing pancreatic cancer from healthy and pancreatitis conditions using both internally held metabolites and externally excreted modified nucleosides for both diagnosis and screening potential. Further experimental analysis using SILAC to trace glucose and glutamine use by cancerous and healthy cells has been used to elucidate differences in energy resource utilisation. Chapter 3- Looks at the development of a metabolomic analysis capable of distinguishing various serum-based conditions from each other as well as analysing metabolic profile changes between males, females (pregnant and non-pregnant) and cord samples to establish key profile changes that occur in pregnancy as well as establishing how these changes may be conveyed in breast-milk production to further support baby development after birth. Chapter 4- Captures faecal markers capable of distinguishing successfully from unsuccessfully reproducing captive elephant, seeking diagnostic and explanatory, indicating potential upstream mis-regulations that could be causative of the ailment, markers aiding to potential treatments or future preventions to aid reproduction. Chapter 5- Evaluates drosophila fly as a suitable model for future neurological studies including Alzheimer’s disease. This study has successfully identified numerous lipids, including plasmalogens, in fly heads that make them a suitable candidate in future studies as well as showing the lipidomic changes resultant of disrupted metabolic pathways associated with Alzheimer’s disease. Chapter 6- Establishes the relationship between taurine consumption and exercise performance relating to thermoregulation, through detecting and analysing taurine’s presence in plasma samples taken before and after exercise periods.
published_date 2025-06-10T05:29:06Z
_version_ 1851097923253698560
score 11.089386

Similar Items