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Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach

Sebnem Hazal Gulsen, Evren Tileklioglu, Edna Bode, Harun Cimen, Hatice Ertabaklar, Derya Ulug, Sema Ertug, Sebastian L. Wenski, Mustapha Touray Orcid Logo, Canan Hazir, Duygu Kaya Bilecenoglu, Ibrahim Yildiz, Helge B. Bode, Selcuk Hazir

Scientific Reports, Volume: 12, Issue: 1

Swansea University Author: Mustapha Touray Orcid Logo

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DOI (Published version): 10.1038/s41598-022-13722-z

Abstract

Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the a...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: Springer Science and Business Media LLC 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa69477
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spelling 2025-06-16T12:27:11.3571190 v2 69477 2025-05-08 Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach 525f9e9af0d60813fdaee65dc0cb7cdf 0000-0002-9550-0782 Mustapha Touray Mustapha Touray true false 2025-05-08 BGPS Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future. Journal Article Scientific Reports 12 1 Springer Science and Business Media LLC 2045-2322 24 6 2022 2022-06-24 10.1038/s41598-022-13722-z COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University Other Open Access funding enabled and organized by Projekt DEAL. This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK-Project Number: 116S387) and Aydin Adnan Menderes University, Project Number: 20001). Work in the Bode lab was supported by the BMBF (Project Number: 01DL17009) and the LOEWE Center TBG funded by the state of Hesse. 2025-06-16T12:27:11.3571190 2025-05-08T23:03:19.1957867 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Sebnem Hazal Gulsen 1 Evren Tileklioglu 2 Edna Bode 3 Harun Cimen 4 Hatice Ertabaklar 5 Derya Ulug 6 Sema Ertug 7 Sebastian L. Wenski 8 Mustapha Touray 0000-0002-9550-0782 9 Canan Hazir 10 Duygu Kaya Bilecenoglu 11 Ibrahim Yildiz 12 Helge B. Bode 13 Selcuk Hazir 14 69477__34480__6ca1770a81ab4f8bb1b555aa9364ef18.pdf 69477.VoR.pdf 2025-06-13T16:21:15.9188839 Output 1904188 application/pdf Version of Record true © The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International License. true eng http://creativecommons.org/licenses/by/4.0/
title Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
spellingShingle Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
Mustapha Touray
title_short Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
title_full Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
title_fullStr Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
title_full_unstemmed Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
title_sort Antiprotozoal activity of different Xenorhabdus and Photorhabdus bacterial secondary metabolites and identification of bioactive compounds using the easyPACId approach
author_id_str_mv 525f9e9af0d60813fdaee65dc0cb7cdf
author_id_fullname_str_mv 525f9e9af0d60813fdaee65dc0cb7cdf_***_Mustapha Touray
author Mustapha Touray
author2 Sebnem Hazal Gulsen
Evren Tileklioglu
Edna Bode
Harun Cimen
Hatice Ertabaklar
Derya Ulug
Sema Ertug
Sebastian L. Wenski
Mustapha Touray
Canan Hazir
Duygu Kaya Bilecenoglu
Ibrahim Yildiz
Helge B. Bode
Selcuk Hazir
format Journal article
container_title Scientific Reports
container_volume 12
container_issue 1
publishDate 2022
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-022-13722-z
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.
published_date 2022-06-24T05:28:18Z
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