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Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts

Selçuk Hazir Orcid Logo, Harry K. Kaya Orcid Logo, Mustapha Touray Orcid Logo, Harun Çimen Orcid Logo, David Shapiro-Ilan Orcid Logo

Turkish Journal of Zoology, Volume: 46, Issue: 4, Pages: 305 - 350

Swansea University Author: Mustapha Touray Orcid Logo

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DOI (Published version): 10.55730/1300-0179.3085

Abstract

Broad spectrum chemical pesticides are harmful to humans and other nontarget organisms. Biological control, which entails the use of natural enemies, is a viable alternative. Isolation, identification, and application of biocontrol agents such as the entomopathogenic nematodes (EPNs), Steinernema an...

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Published in: Turkish Journal of Zoology
ISSN: 1300-0179 1303-6114
Published: Scientific and Technological Research Council of Türkiye (TÜBİTAK) 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa69463
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spelling 2025-06-17T13:01:38.4109767 v2 69463 2025-05-08 Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts 525f9e9af0d60813fdaee65dc0cb7cdf 0000-0002-9550-0782 Mustapha Touray Mustapha Touray true false 2025-05-08 BGPS Broad spectrum chemical pesticides are harmful to humans and other nontarget organisms. Biological control, which entails the use of natural enemies, is a viable alternative. Isolation, identification, and application of biocontrol agents such as the entomopathogenic nematodes (EPNs), Steinernema and Heterorhabditis and their symbiotic bacteria, Xenorhabdus and Photorhabdus have increased substantially over the last four decades, and the trend continues with advancement of molecular techniques. Yet, there is a need for a simple hands-on guide for their proper identification, classification, and handling, especially for researchers and users who are not totally familiar with these biocontrol agents. Thus, this manual is intended to provide a practical guide for students and researchers interested in or wanting to focus on these organisms. The manual describes the general biology and bionomics of these nematode/bacterium complexes and explains various basic standard protocols and methods frequently used in research and field laboratories ranging from isolation to application methods. Methods for rearing the insects, Galleria mellonella and Tenebrio molitor, which are routinely used in bioassays and recovery of EPNs, are also included. Journal Article Turkish Journal of Zoology 46 4 305 350 Scientific and Technological Research Council of Türkiye (TÜBİTAK) 1300-0179 1303-6114 Biological control, insect-parasitic nematodes, Steinernema, Heterorhabditis, basic methods, Photorhabdus, Xenorhabdus, standard protocols 6 7 2022 2022-07-06 10.55730/1300-0179.3085 Review COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University Not Required 2025-06-17T13:01:38.4109767 2025-05-08T22:47:37.5257518 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Selçuk Hazir 0000-0001-9298-1472 1 Harry K. Kaya 0000-0001-6742-7779 2 Mustapha Touray 0000-0002-9550-0782 3 Harun Çimen 0000-0002-0106-4183 4 David Shapiro-Ilan 0000-0002-5666-4848 5 69463__34494__b3eecb9ca9b24c2b8666b1fe0f5ce25c.pdf 69463.VOR.pdf 2025-06-17T12:57:13.3975235 Output 8440460 application/pdf Version of Record true This work is licensed under a Creative Commons Attribution 4.0 International License. true eng https://creativecommons.org/licenses/by/4.0/
title Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
spellingShingle Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
Mustapha Touray
title_short Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
title_full Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
title_fullStr Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
title_full_unstemmed Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
title_sort Basic laboratory and field manual for conducting research with the entomopathogenic nematodes, Steinernema and Heterorhabditis, and their bacterial symbionts
author_id_str_mv 525f9e9af0d60813fdaee65dc0cb7cdf
author_id_fullname_str_mv 525f9e9af0d60813fdaee65dc0cb7cdf_***_Mustapha Touray
author Mustapha Touray
author2 Selçuk Hazir
Harry K. Kaya
Mustapha Touray
Harun Çimen
David Shapiro-Ilan
format Journal article
container_title Turkish Journal of Zoology
container_volume 46
container_issue 4
container_start_page 305
publishDate 2022
institution Swansea University
issn 1300-0179
1303-6114
doi_str_mv 10.55730/1300-0179.3085
publisher Scientific and Technological Research Council of Türkiye (TÜBİTAK)
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Broad spectrum chemical pesticides are harmful to humans and other nontarget organisms. Biological control, which entails the use of natural enemies, is a viable alternative. Isolation, identification, and application of biocontrol agents such as the entomopathogenic nematodes (EPNs), Steinernema and Heterorhabditis and their symbiotic bacteria, Xenorhabdus and Photorhabdus have increased substantially over the last four decades, and the trend continues with advancement of molecular techniques. Yet, there is a need for a simple hands-on guide for their proper identification, classification, and handling, especially for researchers and users who are not totally familiar with these biocontrol agents. Thus, this manual is intended to provide a practical guide for students and researchers interested in or wanting to focus on these organisms. The manual describes the general biology and bionomics of these nematode/bacterium complexes and explains various basic standard protocols and methods frequently used in research and field laboratories ranging from isolation to application methods. Methods for rearing the insects, Galleria mellonella and Tenebrio molitor, which are routinely used in bioassays and recovery of EPNs, are also included.
published_date 2022-07-06T05:28:15Z
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