Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Touray, Mustapha; Cimen, Harun; Bode, Edna; Bode, Helge B.; Hazir, Selcuk

doi:10.1007/s10340-024-01760-7

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Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

Mustapha Touray

, Harun Cimen, Edna Bode, Helge B. Bode, Selcuk Hazir

Journal of Pest Science, Volume: 97, Issue: 4, Pages: 2203 - 2215

Swansea University Author: Mustapha Touray

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DOI (Published version): 10.1007/s10340-024-01760-7

Abstract

Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial s...

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Published in:	Journal of Pest Science
ISSN:	1612-4758 1612-4766
Published:	Springer Science and Business Media LLC 2024
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa69458

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Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. 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spelling	2025-06-17T14:57:56.3256497 v2 69458 2025-05-08 Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus 525f9e9af0d60813fdaee65dc0cb7cdf 0000-0002-9550-0782 Mustapha Touray Mustapha Touray true false 2025-05-08 BGPS Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such deterrents targeting egg-laying could be valuable for controlling populations by disrupting their breeding in suitable habitats. Journal Article Journal of Pest Science 97 4 2203 2215 Springer Science and Business Media LLC 1612-4758 1612-4766 Oviposition; Deterrence; Mosquito; Xenorhabdus; Photorhabdus; Fabclavines; easyPACId method 1 9 2024 2024-09-01 10.1007/s10340-024-01760-7 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) Open access funding provided by the Scientific and Technological Research Council of Türkiye (TÜBİTAK). Work in Hazir lab was funded by Aydin Adnan Menderes University (project no. FEF-22014) and TUBITAK-116Z074. Work in Bode lab was supported by LOEWE TBG funded by the state of Hesse and an ERC Advanced Grant (835108). 2025-06-17T14:57:56.3256497 2025-05-08T22:44:15.9931724 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Mustapha Touray 0000-0002-9550-0782 1 Harun Cimen 2 Edna Bode 3 Helge B. Bode 4 Selcuk Hazir 5 69458__34496__d1bcedaeaf964bbea2002ed196709196.pdf 69458.VOR.pdf 2025-06-17T14:00:06.1551663 Output 1702155 application/pdf Version of Record true © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License. true eng http://creativecommons.org/licenses/by/4.0/
title	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
spellingShingle	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus Mustapha Touray
title_short	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
title_full	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
title_fullStr	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
title_full_unstemmed	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
title_sort	Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus
author_id_str_mv	525f9e9af0d60813fdaee65dc0cb7cdf
author_id_fullname_str_mv	525f9e9af0d60813fdaee65dc0cb7cdf_***_Mustapha Touray
author	Mustapha Touray
author2	Mustapha Touray Harun Cimen Edna Bode Helge B. Bode Selcuk Hazir
format	Journal article
container_title	Journal of Pest Science
container_volume	97
container_issue	4
container_start_page	2203
publishDate	2024
institution	Swansea University
issn	1612-4758 1612-4766
doi_str_mv	10.1007/s10340-024-01760-7
publisher	Springer Science and Business Media LLC
college_str	Faculty of Science and Engineering
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description	Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such deterrents targeting egg-laying could be valuable for controlling populations by disrupting their breeding in suitable habitats.
published_date	2024-09-01T05:28:14Z
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Effects of Xenorhabdus and Photorhabdus bacterial metabolites on the ovipositional activity of Aedes albopictus

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