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The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial

Niall Coates Orcid Logo, Daniel A. John, Sue Jordan, Mel Storey, Cathy Thornton Orcid Logo, Iveta Garaiova Orcid Logo, Duolao Wang Orcid Logo, Stephen J. Allen Orcid Logo, Daryn R. Michael Orcid Logo, Susan F. Plummer, Paul Facey Orcid Logo

Microorganisms, Volume: 13, Issue: 5, Start page: 984

Swansea University Authors: Sue Jordan, Mel Storey, Cathy Thornton Orcid Logo, Paul Facey Orcid Logo

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DOI (Published version): 10.3390/microorganisms13050984

Abstract

Early-life establishment of the gut microbiota plays a role in lifelong health, with disruptions linked to heightened risks of metabolic and immune disorders. Probiotic supplementation may be used to modulate the infant gut microbiome to promote favourable development. Here, we evaluate how Lab4B pr...

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Published in: Microorganisms
ISSN: 2076-2607
Published: MDPI AG 2025
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URI: https://cronfa.swan.ac.uk/Record/cronfa69374
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spelling 2025-05-02T16:42:05.8459333 v2 69374 2025-04-30 The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial 24ce9db29b4bde1af4e83b388aae0ea1 Sue Jordan Sue Jordan true false adcafef962a8fc2f0a14d26215225b9c Mel Storey Mel Storey true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false dc25910b8004b2694df68ed7426e1286 0000-0002-3229-0255 Paul Facey Paul Facey true false 2025-04-30 Early-life establishment of the gut microbiota plays a role in lifelong health, with disruptions linked to heightened risks of metabolic and immune disorders. Probiotic supplementation may be used to modulate the infant gut microbiome to promote favourable development. Here, we evaluate how Lab4B probiotic supplementation shapes the development of the infant gut microbiome over the first 6 months. Faecal samples collected from infants enrolled in PROBAT (ISRCTN26287422), a randomised, double-blind, placebo-controlled trial, were analysed using culture-dependent and -independent (16S rDNA and metagenomic shotgun sequencing) techniques to examine the composition, diversity, and metabolic capabilities of the microbiome, as well as the abundance of antimicrobial resistance genes (ARGs). Probiotic supplementation encouraged the development of a microbiome with a distinct composition characterised by elevated abundances of Bifidobacteriaceae in the first 6 weeks (p = 0.006) and Lactobacillaceae throughout the first 6 months (p < 0.05 at every 6-week time point), accelerated microbial diversification, reduced abundance of beta-lactam- and cephalosporin-resistance genes, and differences in predicted metabolic capabilities at the start and end points. Supplementation of this neonatal population, which is at high risk of atopy, with the Lab4B probiotic significantly influenced the development of the infant gut microbiota during the first 6 months. Journal Article Microorganisms 13 5 984 MDPI AG 2076-2607 infant gut microbiota development; multi-strain probiotic; atopy; DNA sequencing 25 4 2025 2025-04-25 10.3390/microorganisms13050984 COLLEGE NANME COLLEGE CODE Swansea University Another institution paid the OA fee This research was funded by the Knowledge Exploitation Fund, Collaborative Industrial Research (Project No. HE09 COL 1002), European Regional Development Fund (ERDF) Accelerate Programme (Project code: PR-0228) and Cultech Ltd. (Port Talbot, UK). 2025-05-02T16:42:05.8459333 2025-04-30T15:14:27.0532533 Faculty of Medicine, Health and Life Sciences School of Health and Social Care - Nursing Niall Coates 0009-0001-3287-2095 1 Daniel A. John 2 Sue Jordan 3 Mel Storey 4 Cathy Thornton 0000-0002-5153-573X 5 Iveta Garaiova 0000-0002-8830-1101 6 Duolao Wang 0000-0003-2788-2464 7 Stephen J. Allen 0000-0001-6675-249x 8 Daryn R. Michael 0000-0001-8546-1153 9 Susan F. Plummer 10 Paul Facey 0000-0002-3229-0255 11 69374__34182__5c975965af2c410991b43b3fdbedb3c4.pdf 69374.VoR.pdf 2025-05-02T16:40:02.5890205 Output 9327228 application/pdf Version of Record true © 2025 by the authors. This is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. true eng https://creativecommons.org/ licenses/by/4.0/
title The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
spellingShingle The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
Sue Jordan
Mel Storey
Cathy Thornton
Paul Facey
title_short The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
title_full The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
title_fullStr The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
title_full_unstemmed The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
title_sort The Impact of Probiotic Supplementation on the Development of the Infant Gut Microbiota: An Exploratory Follow-Up of a Randomised Controlled Trial
author_id_str_mv 24ce9db29b4bde1af4e83b388aae0ea1
adcafef962a8fc2f0a14d26215225b9c
c71a7a4be7361094d046d312202bce0c
dc25910b8004b2694df68ed7426e1286
author_id_fullname_str_mv 24ce9db29b4bde1af4e83b388aae0ea1_***_Sue Jordan
adcafef962a8fc2f0a14d26215225b9c_***_Mel Storey
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
dc25910b8004b2694df68ed7426e1286_***_Paul Facey
author Sue Jordan
Mel Storey
Cathy Thornton
Paul Facey
author2 Niall Coates
Daniel A. John
Sue Jordan
Mel Storey
Cathy Thornton
Iveta Garaiova
Duolao Wang
Stephen J. Allen
Daryn R. Michael
Susan F. Plummer
Paul Facey
format Journal article
container_title Microorganisms
container_volume 13
container_issue 5
container_start_page 984
publishDate 2025
institution Swansea University
issn 2076-2607
doi_str_mv 10.3390/microorganisms13050984
publisher MDPI AG
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str School of Health and Social Care - Nursing{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}School of Health and Social Care - Nursing
document_store_str 1
active_str 0
description Early-life establishment of the gut microbiota plays a role in lifelong health, with disruptions linked to heightened risks of metabolic and immune disorders. Probiotic supplementation may be used to modulate the infant gut microbiome to promote favourable development. Here, we evaluate how Lab4B probiotic supplementation shapes the development of the infant gut microbiome over the first 6 months. Faecal samples collected from infants enrolled in PROBAT (ISRCTN26287422), a randomised, double-blind, placebo-controlled trial, were analysed using culture-dependent and -independent (16S rDNA and metagenomic shotgun sequencing) techniques to examine the composition, diversity, and metabolic capabilities of the microbiome, as well as the abundance of antimicrobial resistance genes (ARGs). Probiotic supplementation encouraged the development of a microbiome with a distinct composition characterised by elevated abundances of Bifidobacteriaceae in the first 6 weeks (p = 0.006) and Lactobacillaceae throughout the first 6 months (p < 0.05 at every 6-week time point), accelerated microbial diversification, reduced abundance of beta-lactam- and cephalosporin-resistance genes, and differences in predicted metabolic capabilities at the start and end points. Supplementation of this neonatal population, which is at high risk of atopy, with the Lab4B probiotic significantly influenced the development of the infant gut microbiota during the first 6 months.
published_date 2025-04-25T05:29:53Z
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score 11.096068

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