No Cover Image

Journal article 380 views 71 downloads

Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor

Thabang B. M. Mosaka, John O. Unuofin Orcid Logo, Michael O. Daramola, Chedly Tizaoui Orcid Logo, Samuel A. Iwarere

Environmental Science and Pollution Research, Volume: 31, Issue: 37, Pages: 49811 - 49822

Swansea University Author: Chedly Tizaoui Orcid Logo

  • 69315.VoR.pdf

    PDF | Version of Record

    © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License.

    Download (1.33MB)

Check full text

DOI (Published version): 10.1007/s11356-024-34475-4

Abstract

Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the AR...

Full description

Published in: Environmental Science and Pollution Research
ISSN: 1614-7499
Published: Springer Science and Business Media LLC 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa69315
first_indexed 2025-04-21T16:01:37Z
last_indexed 2025-05-13T09:11:11Z
id cronfa69315
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2025-05-12T12:20:26.9582619</datestamp><bib-version>v2</bib-version><id>69315</id><entry>2025-04-21</entry><title>Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor</title><swanseaauthors><author><sid>4b34a0286d3c0b0b081518fa6987031d</sid><ORCID>0000-0003-2159-7881</ORCID><firstname>Chedly</firstname><surname>Tizaoui</surname><name>Chedly Tizaoui</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2025-04-21</date><deptcode>EAAS</deptcode><abstract>Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of&#x2009;&#x2265;&#x2009;8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs.</abstract><type>Journal Article</type><journal>Environmental Science and Pollution Research</journal><volume>31</volume><journalNumber>37</journalNumber><paginationStart>49811</paginationStart><paginationEnd>49822</paginationEnd><publisher>Springer Science and Business Media LLC</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>1614-7499</issnElectronic><keywords>Acinetobacter baumannii; Carbapenem-resistant gene; Cold atmospheric plasma; Disinfection</keywords><publishedDay>31</publishedDay><publishedMonth>7</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-07-31</publishedDate><doi>10.1007/s11356-024-34475-4</doi><url/><notes/><college>COLLEGE NANME</college><department>Engineering and Applied Sciences School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>EAAS</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>Open access funding provided by University of Pretoria. This research was supported by the Government of the United Kingdom through The Royal Society FLAIR award [FLR\R1\201683].</funders><projectreference/><lastEdited>2025-05-12T12:20:26.9582619</lastEdited><Created>2025-04-21T12:34:46.7249023</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Chemical Engineering</level></path><authors><author><firstname>Thabang B. M.</firstname><surname>Mosaka</surname><order>1</order></author><author><firstname>John O.</firstname><surname>Unuofin</surname><orcid>0000-0002-8096-1156</orcid><order>2</order></author><author><firstname>Michael O.</firstname><surname>Daramola</surname><order>3</order></author><author><firstname>Chedly</firstname><surname>Tizaoui</surname><orcid>0000-0003-2159-7881</orcid><order>4</order></author><author><firstname>Samuel A.</firstname><surname>Iwarere</surname><order>5</order></author></authors><documents><document><filename>69315__34247__645687e208dc40f6b42fd9e412d7b39d.pdf</filename><originalFilename>69315.VoR.pdf</originalFilename><uploaded>2025-05-12T12:12:22.3670393</uploaded><type>Output</type><contentLength>1397119</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2025-05-12T12:20:26.9582619 v2 69315 2025-04-21 Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor 4b34a0286d3c0b0b081518fa6987031d 0000-0003-2159-7881 Chedly Tizaoui Chedly Tizaoui true false 2025-04-21 EAAS Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs. Journal Article Environmental Science and Pollution Research 31 37 49811 49822 Springer Science and Business Media LLC 1614-7499 Acinetobacter baumannii; Carbapenem-resistant gene; Cold atmospheric plasma; Disinfection 31 7 2024 2024-07-31 10.1007/s11356-024-34475-4 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee Open access funding provided by University of Pretoria. This research was supported by the Government of the United Kingdom through The Royal Society FLAIR award [FLR\R1\201683]. 2025-05-12T12:20:26.9582619 2025-04-21T12:34:46.7249023 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Thabang B. M. Mosaka 1 John O. Unuofin 0000-0002-8096-1156 2 Michael O. Daramola 3 Chedly Tizaoui 0000-0003-2159-7881 4 Samuel A. Iwarere 5 69315__34247__645687e208dc40f6b42fd9e412d7b39d.pdf 69315.VoR.pdf 2025-05-12T12:12:22.3670393 Output 1397119 application/pdf Version of Record true © The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License. true eng http://creativecommons.org/licenses/by/4.0/
title Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
spellingShingle Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
Chedly Tizaoui
title_short Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
title_full Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
title_fullStr Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
title_full_unstemmed Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
title_sort Non-thermal obliteration of critically ranked carbapenem-resistant Acinetobacter baumannii and its resistance gene in a batch atmospheric plasma reactor
author_id_str_mv 4b34a0286d3c0b0b081518fa6987031d
author_id_fullname_str_mv 4b34a0286d3c0b0b081518fa6987031d_***_Chedly Tizaoui
author Chedly Tizaoui
author2 Thabang B. M. Mosaka
John O. Unuofin
Michael O. Daramola
Chedly Tizaoui
Samuel A. Iwarere
format Journal article
container_title Environmental Science and Pollution Research
container_volume 31
container_issue 37
container_start_page 49811
publishDate 2024
institution Swansea University
issn 1614-7499
doi_str_mv 10.1007/s11356-024-34475-4
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
active_str 0
description Wastewater treatment plants (WWTPs) have been implicated as direct key reservoir of both antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) associated with human infection, as high concentrations of ARBs and ARGs have been detected in recycled hospital wastewater. Among the ARBs, the carbapenem-resistant Acinetobacter baumannii has been ranked as priority 1 (critical) pathogen by the World Health Organization (WHO), due to its overwhelming burden on public health. Therefore, this study is aimed at investigating non-thermal plasma (NTP) technology as an alternative disinfection step to inactivate this bacterium and its ARGs. Culture-based method and PCR were employed in confirming the carbapenem resistance gene blaNDM-1 in A. baumannii (BAA 1605). Suspension of carbapenem-resistant A. baumannii (24 h culture) was prepared from the confirmed isolate and subjected to plasma treatment at varying time intervals (3 min, 6 min, 9 min, 12 min, and 15 min) in triplicates. The plasma-treated samples were evaluated for re-growth and the presence of the resistance gene. The treatment resulted in a 1.13 log reduction after 3 min and the highest log reduction of ≥ 8 after 15 min, and the results also showed that NTP was able to inactivate the blaNDM-1 gene. The log reduction and gel image results suggest that plasma disinfection has a great potential to be an efficient tertiary treatment step for WWTPs.
published_date 2024-07-31T05:27:50Z
_version_ 1851097844018053120
score 11.089386

Similar Items