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Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts

Salvatore Vaiasicca, David James, GIANMARCO MELONE, OMAR SAEED, Lewis Francis Orcid Logo, Bruna Corradetti

Stem Cell Research & Therapy, Volume: 16, Start page: 151

Swansea University Authors: David James, GIANMARCO MELONE, OMAR SAEED, Lewis Francis Orcid Logo, Bruna Corradetti

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Abstract

Several immunosuppressive therapies have been proposed as key treatment options for critically ill patients since the first appearance of severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) from different sources have been considered for their potential to attenuate the cy...

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Published in: Stem Cell Research & Therapy
ISSN: 1757-6512
Published: Springer Nature 2025
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URI: https://cronfa.swan.ac.uk/Record/cronfa69273
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spelling 2025-04-11T12:33:44.0006058 v2 69273 2025-04-11 Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts 31b39419835be9525450cf1420e63996 David James David James true false 32bf44fef9e3d7d7ac3a05106898d3f5 GIANMARCO MELONE GIANMARCO MELONE true true 324097be6a7ee14699388db59b5c5066 OMAR SAEED OMAR SAEED true true 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false aa6a235c9e53c5b9b00e751422db5277 Bruna Corradetti Bruna Corradetti true false 2025-04-11 ONDF Several immunosuppressive therapies have been proposed as key treatment options for critically ill patients since the first appearance of severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) from different sources have been considered for their potential to attenuate the cytokine storm associated to COVID-19 and the consequent multi-organ failure, providing evidence for safe and efficacious treatments. Among them, administration of umbilical cord-derived MSCs (UC-MSCs) has demonstrated a significant increase in survival rates, largely due to their potent immunosuppressive properties. We applied next-generation sequencing (NGS) analysis to compare the transcriptomic profiles of MSCs isolated from two gestational sources: amniotic fluid (AF) obtained during prenatal diagnosis and their clinically relevant umbilical cord counterparts, for which datasets were publicly available. A full meta-analysis was performed to identify suitable GEO and NGS datasets for comparison between AF- and UC-MSC samples. Transcriptome analysis revelaed significant differences between groups, despite both cell lines being strongly involved in the tissue development, crucial to achieve the complex task of wound healing. Significantly enriched hallmark genes suggest AF-MSC superior immunomodulatory features against signaling pathways actively involved in the cytokine storm (i.e., IL-2/STAT, TNF-a/NFkB, IL-2/STAT5, PI3K/AKT/mTOR). The data presented here suggest that AF-MSCs hold significant promise for treating not only COVID-19-associated cytokine storms but also a variety of other inflammatory syndromes (i.e., those induced by bacterial infections, autoimmune disorders, and therapeutic interventions). Realizing the full potential of AF-MSCs as a comprehensive therapeutic approach in inflammatory disease management will require more extensive clinical trials and in-depth mechanistic studies. Journal Article Stem Cell Research & Therapy 16 151 Springer Nature 1757-6512 Mesenchymal stem cells; Amniotic fluid; Umbilical cord; Cytokine storm; Transcriptomic analysis; Regulatory moieties; Immunosuppression; GSEA; COVID-19 28 3 2025 2025-03-28 10.1186/s13287-025-04262-0 COLLEGE NANME Other/Subsidiary Companies - Not Defined COLLEGE CODE ONDF Swansea University Another institution paid the OA fee B.C. received salary support from the Sêr Cymru II programme, funded by the European Commission through the Horizon 2020 Marie Skłodowska-Curie Actions (MSCA) COFUND scheme and the Welsh European Funding Office (WEFO) under the European Regional Development Fund (ERDF) (2018–2022). This funding body played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. 2025-04-11T12:33:44.0006058 2025-04-11T10:18:45.4214893 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Salvatore Vaiasicca 1 David James 2 GIANMARCO MELONE 3 OMAR SAEED 4 Lewis Francis 0000-0002-7803-7714 5 Bruna Corradetti 6 69273__34015__b6903da53e9a4e50bc4279271035fd7e.pdf 69273.VOR.pdf 2025-04-11T12:06:41.1246978 Output 5172160 application/pdf Version of Record true © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). true eng http://creativecommons.org/licenses/by/4.0/
title Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
spellingShingle Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
David James
GIANMARCO MELONE
OMAR SAEED
Lewis Francis
Bruna Corradetti
title_short Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
title_full Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
title_fullStr Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
title_full_unstemmed Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
title_sort Amniotic fluid-derived mesenchymal stem cells as a therapeutic tool against cytokine storm: a comparison with umbilical cord counterparts
author_id_str_mv 31b39419835be9525450cf1420e63996
32bf44fef9e3d7d7ac3a05106898d3f5
324097be6a7ee14699388db59b5c5066
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aa6a235c9e53c5b9b00e751422db5277
author_id_fullname_str_mv 31b39419835be9525450cf1420e63996_***_David James
32bf44fef9e3d7d7ac3a05106898d3f5_***_GIANMARCO MELONE
324097be6a7ee14699388db59b5c5066_***_OMAR SAEED
10f61f9c1248951c1a33f6a89498f37d_***_Lewis Francis
aa6a235c9e53c5b9b00e751422db5277_***_Bruna Corradetti
author David James
GIANMARCO MELONE
OMAR SAEED
Lewis Francis
Bruna Corradetti
author2 Salvatore Vaiasicca
David James
GIANMARCO MELONE
OMAR SAEED
Lewis Francis
Bruna Corradetti
format Journal article
container_title Stem Cell Research & Therapy
container_volume 16
container_start_page 151
publishDate 2025
institution Swansea University
issn 1757-6512
doi_str_mv 10.1186/s13287-025-04262-0
publisher Springer Nature
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description Several immunosuppressive therapies have been proposed as key treatment options for critically ill patients since the first appearance of severe acute respiratory syndrome coronavirus 2. Mesenchymal stem cells (MSCs) from different sources have been considered for their potential to attenuate the cytokine storm associated to COVID-19 and the consequent multi-organ failure, providing evidence for safe and efficacious treatments. Among them, administration of umbilical cord-derived MSCs (UC-MSCs) has demonstrated a significant increase in survival rates, largely due to their potent immunosuppressive properties. We applied next-generation sequencing (NGS) analysis to compare the transcriptomic profiles of MSCs isolated from two gestational sources: amniotic fluid (AF) obtained during prenatal diagnosis and their clinically relevant umbilical cord counterparts, for which datasets were publicly available. A full meta-analysis was performed to identify suitable GEO and NGS datasets for comparison between AF- and UC-MSC samples. Transcriptome analysis revelaed significant differences between groups, despite both cell lines being strongly involved in the tissue development, crucial to achieve the complex task of wound healing. Significantly enriched hallmark genes suggest AF-MSC superior immunomodulatory features against signaling pathways actively involved in the cytokine storm (i.e., IL-2/STAT, TNF-a/NFkB, IL-2/STAT5, PI3K/AKT/mTOR). The data presented here suggest that AF-MSCs hold significant promise for treating not only COVID-19-associated cytokine storms but also a variety of other inflammatory syndromes (i.e., those induced by bacterial infections, autoimmune disorders, and therapeutic interventions). Realizing the full potential of AF-MSCs as a comprehensive therapeutic approach in inflammatory disease management will require more extensive clinical trials and in-depth mechanistic studies.
published_date 2025-03-28T05:39:04Z
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