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Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria / ALEXANDRA GIMBLETT

Swansea University Author: ALEXANDRA GIMBLETT

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Abstract

Microalgal wastewater treatment systems may solve several problems with traditional systems. They can cope with high nutrient loads, do not require a supplementary oxygen input, and contribute to a circular economy. However, there are still challenges presented when upscaling these systems. Certain...

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Published: Swansea University, Wales, UK 2025
Institution: Swansea University
Degree level: Master of Research
Degree name: MRes
Supervisor: Sonnenschein, E., and Webster, T. U.
URI: https://cronfa.swan.ac.uk/Record/cronfa68885
first_indexed 2025-02-13T16:01:50Z
last_indexed 2025-02-14T05:46:34Z
id cronfa68885
recordtype RisThesis
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spelling 2025-02-13T16:08:52.2882263 v2 68885 2025-02-13 Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria 2b4c213aa5a25d2d2a58715cd940343b ALEXANDRA GIMBLETT ALEXANDRA GIMBLETT true false 2025-02-13 Microalgal wastewater treatment systems may solve several problems with traditional systems. They can cope with high nutrient loads, do not require a supplementary oxygen input, and contribute to a circular economy. However, there are still challenges presented when upscaling these systems. Certain bacterial strains can promote microalgal growth due to the mutualistic symbioticrelationships formed between these microbes. Therefore, the purpose of this study was to analyse microalgal microbiomes that have been cultivated using wastewater and identify potentially beneficial bacteria within these microbiomes with the aim to make microalgal wastewater treatment systems viable at large scales. DNA extraction, 16S rRNA amplicon sequencing, and co-cultivation experiments were conducted to analyse the biodiversity of the Tetradesmus obliquus microbiome when cultivated on agricultural digestate, identify possible beneficial bacterial strains for use withinT. obliquus wastewater treatment systems, and investigate the impact of specific bacterial strains onT. obliquus growth. The T. obliquus microbiomes cultivated on digestate had significantly different bacterial community compositions with lower species diversity and evenness compared to the T. obliquus microbiomes cultivated using a control medium. A bacterial strain belonging to the Pseudomonadaceae family was found in great abundance within the digestate-treated microbiomes and appeared to rapidly grow in abundance within this environment after the first day of cultivation. Co-cultivation analysis found that an isolate identified as Psuedomonas laurentiana, which shared significant sequence similarity to the bacterial strain belonging to the Pseudomonadaceae family, significantly increased T. obliquus growth. The isolate identified as P. laurentiana may therefore be suitable for use within and advance T. obliquus wastewater treatment systems. Further co-cultivation studies should be conducted within digestate and other wastewater environments at larger scales to determine if the consortia of T. obliquus and P. laurentiana are effective when trying to upscale T. obliquus wastewater treatment systems and make them more efficient. E-Thesis Swansea University, Wales, UK Microalgae, bacteria, wastewater treatment, interactions. 17 2 2025 2025-02-17 A selection of content is redacted or is partially redacted from this thesis to protect sensitive and personal information. COLLEGE NANME COLLEGE CODE Swansea University Sonnenschein, E., and Webster, T. U. Master of Research MRes 2025-02-13T16:08:52.2882263 2025-02-13T15:35:49.9995651 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences ALEXANDRA GIMBLETT 1 68885__33585__28fa644a3a1046e89d579ed57090e8aa.pdf 2024_Gimblett_A.final.68885.pdf 2025-02-13T16:04:25.4062370 Output 4227504 application/pdf E-Thesis – open access true Copyright: The Author, Alexandra Gimblett, 2024 Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0) true eng https://creativecommons.org/licenses/by/4.0/
title Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
spellingShingle Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
ALEXANDRA GIMBLETT
title_short Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
title_full Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
title_fullStr Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
title_full_unstemmed Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
title_sort Finding new biotechnological solutions - Advancing microalgal wastewater treatment with beneficial bacteria
author_id_str_mv 2b4c213aa5a25d2d2a58715cd940343b
author_id_fullname_str_mv 2b4c213aa5a25d2d2a58715cd940343b_***_ALEXANDRA GIMBLETT
author ALEXANDRA GIMBLETT
author2 ALEXANDRA GIMBLETT
format E-Thesis
publishDate 2025
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Microalgal wastewater treatment systems may solve several problems with traditional systems. They can cope with high nutrient loads, do not require a supplementary oxygen input, and contribute to a circular economy. However, there are still challenges presented when upscaling these systems. Certain bacterial strains can promote microalgal growth due to the mutualistic symbioticrelationships formed between these microbes. Therefore, the purpose of this study was to analyse microalgal microbiomes that have been cultivated using wastewater and identify potentially beneficial bacteria within these microbiomes with the aim to make microalgal wastewater treatment systems viable at large scales. DNA extraction, 16S rRNA amplicon sequencing, and co-cultivation experiments were conducted to analyse the biodiversity of the Tetradesmus obliquus microbiome when cultivated on agricultural digestate, identify possible beneficial bacterial strains for use withinT. obliquus wastewater treatment systems, and investigate the impact of specific bacterial strains onT. obliquus growth. The T. obliquus microbiomes cultivated on digestate had significantly different bacterial community compositions with lower species diversity and evenness compared to the T. obliquus microbiomes cultivated using a control medium. A bacterial strain belonging to the Pseudomonadaceae family was found in great abundance within the digestate-treated microbiomes and appeared to rapidly grow in abundance within this environment after the first day of cultivation. Co-cultivation analysis found that an isolate identified as Psuedomonas laurentiana, which shared significant sequence similarity to the bacterial strain belonging to the Pseudomonadaceae family, significantly increased T. obliquus growth. The isolate identified as P. laurentiana may therefore be suitable for use within and advance T. obliquus wastewater treatment systems. Further co-cultivation studies should be conducted within digestate and other wastewater environments at larger scales to determine if the consortia of T. obliquus and P. laurentiana are effective when trying to upscale T. obliquus wastewater treatment systems and make them more efficient.
published_date 2025-02-17T05:37:56Z
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score 11.379038

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