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Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment

Marcos F. Martinez-Moreno, Cristina Povedano-Priego, Mar Morales-Hidalgo, Adam Mumford, Guillermo Lazuen-Lopez, Elisabet Aranda, Ramiro Vilchez-Vargas, Pier L. Solari, Yon Ju-Nam Orcid Logo, Fadwa Jroundi, Jesus Ojeda Ledo Orcid Logo, Mohamed L. Merroun

Science of The Total Environment, Volume: 965, Start page: 178613

Swansea University Authors: Adam Mumford, Yon Ju-Nam Orcid Logo, Jesus Ojeda Ledo Orcid Logo

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DOI (Published version): 10.1016/j.scitotenv.2025.178613

Abstract

Deep Geological Repository design, the internationally safest option for the long term disposal of high-level radioactive waste (HLW), features metal canisters encased in compacted bentonite clay and embedded deep within a host rock. Despite presenting a hostile environment for microorganisms, DGRs...

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Published in: Science of The Total Environment
ISSN: 0048-9697
Published: Elsevier BV 2025
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URI: https://cronfa.swan.ac.uk/Record/cronfa68712
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Despite presenting a hostile environment for microorganisms, DGRs scenarios with favorable microbial activity conditions must be considered for the safety assessment of this disposal. This study investigated the impact of Se(IV), as a natural analogue of 79Se present in the HLW, in anoxic microcosms of bentonite slurry spiked with a bacterial consortium and amended with lactate, acetate, and sulfate as electron donors/acceptor. The addition of the bacterial consortium promoted the rate of Se(IV) reduction to Se(0), while the tyndallization (heat shock) of bentonite slowed this process. Se(IV) reduced the relative abundance of most genera of sulfate-reducing bacteria (SRB), while stimulating the abundance of Se-tolerant bacteria, which played an important role in Se(IV) reduction. Moreover, it was observed that lactate was the preferred electron donor, linking with the production, and subsequent consumption of acetate. X-ray absorption spectroscopy (XAS) and high-resolution transmission electron microscopy (HRTEM) revealed the reduction of Se(IV) forming amorphous Se(0) nanospheres. In addition, HRTEM showed that the biogenic Se(0) undergo a biotransformation to more stable crystalline forms, contributing to the immobilization of Se in the case of HLW release. Additionally, the sulfide generated by the activity of SRB reacted with Cu producing corrosion products (CuxS) on the surface of the copper material.</abstract><type>Journal Article</type><journal>Science of The Total Environment</journal><volume>965</volume><journalNumber/><paginationStart>178613</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>0048-9697</issnPrint><issnElectronic/><keywords>Deep geological repository; Selenium speciation; Bentonite; Bacteria; Copper biocorrosion</keywords><publishedDay>15</publishedDay><publishedMonth>2</publishedMonth><publishedYear>2025</publishedYear><publishedDate>2025-02-15</publishedDate><doi>10.1016/j.scitotenv.2025.178613</doi><url/><notes/><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>The present work was supported by the grant RTI2018&#x2013;101548-B-I00 &#x201C;ERDF A way of making Europe&#x201D; to MLM from the &#x201C;Ministerio de Ciencia, Innovaci&#xF3;n y Universidades&#x201D; (Spanish Government). The project leading to this application has received funding from the European Union&#x2019;s Horizon 2020 research and innovation programme under grant agreement No 847593 to MLM. 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spelling 2025-03-03T11:45:02.7166122 v2 68712 2025-01-20 Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment 950f680b2dfca0e78d8dd229be5babd7 Adam Mumford Adam Mumford true false b219fdfea246d96dfc9c4eecfd60f2a6 0000-0003-2972-8073 Yon Ju-Nam Yon Ju-Nam true false 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2025-01-20 Deep Geological Repository design, the internationally safest option for the long term disposal of high-level radioactive waste (HLW), features metal canisters encased in compacted bentonite clay and embedded deep within a host rock. Despite presenting a hostile environment for microorganisms, DGRs scenarios with favorable microbial activity conditions must be considered for the safety assessment of this disposal. This study investigated the impact of Se(IV), as a natural analogue of 79Se present in the HLW, in anoxic microcosms of bentonite slurry spiked with a bacterial consortium and amended with lactate, acetate, and sulfate as electron donors/acceptor. The addition of the bacterial consortium promoted the rate of Se(IV) reduction to Se(0), while the tyndallization (heat shock) of bentonite slowed this process. Se(IV) reduced the relative abundance of most genera of sulfate-reducing bacteria (SRB), while stimulating the abundance of Se-tolerant bacteria, which played an important role in Se(IV) reduction. Moreover, it was observed that lactate was the preferred electron donor, linking with the production, and subsequent consumption of acetate. X-ray absorption spectroscopy (XAS) and high-resolution transmission electron microscopy (HRTEM) revealed the reduction of Se(IV) forming amorphous Se(0) nanospheres. In addition, HRTEM showed that the biogenic Se(0) undergo a biotransformation to more stable crystalline forms, contributing to the immobilization of Se in the case of HLW release. Additionally, the sulfide generated by the activity of SRB reacted with Cu producing corrosion products (CuxS) on the surface of the copper material. Journal Article Science of The Total Environment 965 178613 Elsevier BV 0048-9697 Deep geological repository; Selenium speciation; Bentonite; Bacteria; Copper biocorrosion 15 2 2025 2025-02-15 10.1016/j.scitotenv.2025.178613 COLLEGE NANME COLLEGE CODE Swansea University Another institution paid the OA fee The present work was supported by the grant RTI2018–101548-B-I00 “ERDF A way of making Europe” to MLM from the “Ministerio de Ciencia, Innovación y Universidades” (Spanish Government). The project leading to this application has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 847593 to MLM. ADM acknowledges funding from the UK Engineering and Physical Sciences Research Council (EPSRC) DTP scholarship (project reference: 2748843). 2025-03-03T11:45:02.7166122 2025-01-20T22:24:50.8527262 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Marcos F. Martinez-Moreno 1 Cristina Povedano-Priego 2 Mar Morales-Hidalgo 3 Adam Mumford 4 Guillermo Lazuen-Lopez 5 Elisabet Aranda 6 Ramiro Vilchez-Vargas 7 Pier L. Solari 8 Yon Ju-Nam 0000-0003-2972-8073 9 Fadwa Jroundi 10 Jesus Ojeda Ledo 0000-0002-2046-1010 11 Mohamed L. Merroun 12 68712__33383__aacf3e96025c42c19726e69494ba7b57.pdf accepted manuscript.pdf 2025-01-20T22:35:36.9812723 Output 14991305 application/pdf Accepted Manuscript true Author accepted manuscript document released under the terms of a Creative Commons CC-BY licence using the Swansea University Research Publications Policy (rights retention). true eng https://creativecommons.org/licenses/by/4.0/deed.en
title Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
spellingShingle Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
Adam Mumford
Yon Ju-Nam
Jesus Ojeda Ledo
title_short Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
title_full Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
title_fullStr Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
title_full_unstemmed Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
title_sort Dual effect of Se(IV) and bentonite microbial community interactions on the corrosion of copper and Se speciation: Implication on repository safety assessment
author_id_str_mv 950f680b2dfca0e78d8dd229be5babd7
b219fdfea246d96dfc9c4eecfd60f2a6
4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv 950f680b2dfca0e78d8dd229be5babd7_***_Adam Mumford
b219fdfea246d96dfc9c4eecfd60f2a6_***_Yon Ju-Nam
4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author Adam Mumford
Yon Ju-Nam
Jesus Ojeda Ledo
author2 Marcos F. Martinez-Moreno
Cristina Povedano-Priego
Mar Morales-Hidalgo
Adam Mumford
Guillermo Lazuen-Lopez
Elisabet Aranda
Ramiro Vilchez-Vargas
Pier L. Solari
Yon Ju-Nam
Fadwa Jroundi
Jesus Ojeda Ledo
Mohamed L. Merroun
format Journal article
container_title Science of The Total Environment
container_volume 965
container_start_page 178613
publishDate 2025
institution Swansea University
issn 0048-9697
doi_str_mv 10.1016/j.scitotenv.2025.178613
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
active_str 0
description Deep Geological Repository design, the internationally safest option for the long term disposal of high-level radioactive waste (HLW), features metal canisters encased in compacted bentonite clay and embedded deep within a host rock. Despite presenting a hostile environment for microorganisms, DGRs scenarios with favorable microbial activity conditions must be considered for the safety assessment of this disposal. This study investigated the impact of Se(IV), as a natural analogue of 79Se present in the HLW, in anoxic microcosms of bentonite slurry spiked with a bacterial consortium and amended with lactate, acetate, and sulfate as electron donors/acceptor. The addition of the bacterial consortium promoted the rate of Se(IV) reduction to Se(0), while the tyndallization (heat shock) of bentonite slowed this process. Se(IV) reduced the relative abundance of most genera of sulfate-reducing bacteria (SRB), while stimulating the abundance of Se-tolerant bacteria, which played an important role in Se(IV) reduction. Moreover, it was observed that lactate was the preferred electron donor, linking with the production, and subsequent consumption of acetate. X-ray absorption spectroscopy (XAS) and high-resolution transmission electron microscopy (HRTEM) revealed the reduction of Se(IV) forming amorphous Se(0) nanospheres. In addition, HRTEM showed that the biogenic Se(0) undergo a biotransformation to more stable crystalline forms, contributing to the immobilization of Se in the case of HLW release. Additionally, the sulfide generated by the activity of SRB reacted with Cu producing corrosion products (CuxS) on the surface of the copper material.
published_date 2025-02-15T12:42:34Z
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score 10.90527

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