The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity

Davies, Nia; Carriere, Julanta; GOPAL, ANEESHA; RAJAN, ANNIE; Wallace, Lisa; Seeley, Aidan

doi:10.1016/j.pbb.2024.173953

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The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity

Nia Davies, Julanta Carriere, ANEESHA GOPAL, ANNIE RAJAN, Lisa Wallace

, Aidan Seeley

Pharmacology Biochemistry and Behavior, Volume: 247, Start page: 173953

Swansea University Authors: Nia Davies, Julanta Carriere, ANEESHA GOPAL, ANNIE RAJAN, Lisa Wallace , Aidan Seeley

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DOI (Published version): 10.1016/j.pbb.2024.173953

Abstract

Nicotine has been shown to induce profound physiological and behavioural responses in invertebrate model organisms such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus is an aquatic oligochaete worm which we have previously demonstrated has application within pharmacolo...

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Published in:	Pharmacology Biochemistry and Behavior
ISSN:	0091-3057 1873-5177
Published:	Elsevier BV 2025
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa68627

first_indexed	2025-01-09T20:34:00Z
last_indexed	2025-01-09T20:34:00Z
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spelling	2025-01-08T14:00:46.0840231 v2 68627 2024-12-31 The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity 977abe5c673627024e4913d034dcbc95 Nia Davies Nia Davies true false 4d91151193de7925ea048bf39f32198b Julanta Carriere Julanta Carriere true false 5e44e9e220512a05e821030192c06160 ANEESHA GOPAL ANEESHA GOPAL true false 8904f590a1fafb850f1bb67cf9d682d2 ANNIE RAJAN ANNIE RAJAN true false 5cebf16bdbc8022118a35da9d13f5087 0000-0001-7471-9833 Lisa Wallace Lisa Wallace true false c69dba86b3ccf9a140b67b7e97d68bba 0000-0001-7085-4296 Aidan Seeley Aidan Seeley true false 2024-12-31 MEDS Nicotine has been shown to induce profound physiological and behavioural responses in invertebrate model organisms such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus is an aquatic oligochaete worm which we have previously demonstrated has application within pharmacological research. Herein, we demonstrate the presence of endogenous acetylcholine and cholinesterase activity within L. variegatus and show the time-dependent effects on the sensitivity of L. variegatus to nicotine. We describe the effects of a broad range of concentrations of nicotine (1 μM – 1 mM) on L. variegatus response to tactile stimulation and locomotor activity following acute (10-min) and chronic (24-h) exposure. Here, we show that 10 min of exposure to ≥0.1 mM nicotine reversibly reduces the ability of tactile stimulation to elicit stereotypical movements of body reversal and helical swimming, and locomotor activity in L. variegatus. We also demonstrate that exposure to ≥0.1 mM nicotine for 24 h was toxic to L. variegatus. Chronic low-dose nicotine ≥25 μM similarly inhibits L. variegatus behaviours with 50 μM causing irreversible inhibition of movement. Thus, L. variegatus presents a model for studying the effects of nicotine and further demonstrates the application of the in vivo model L. variegatus for behavioural pharmacology research. Journal Article Pharmacology Biochemistry and Behavior 247 173953 Elsevier BV 0091-3057 1873-5177 Behavioural pharmacology; Nicotine; Invertebrate; Behavior; Lumbriculus variegatus 1 2 2025 2025-02-01 10.1016/j.pbb.2024.173953 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University SU Library paid the OA fee (TA Institutional Deal) This work was supported by funding from a Saint David's Medical Foundation Seed-corn Research grant 2021 - 2022. 2025-01-08T14:00:46.0840231 2024-12-31T12:19:30.1893647 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Nia Davies 1 Julanta Carriere 2 ANEESHA GOPAL 3 ANNIE RAJAN 4 Lisa Wallace 0000-0001-7471-9833 5 Aidan Seeley 0000-0001-7085-4296 6
title	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
spellingShingle	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity Nia Davies Julanta Carriere ANEESHA GOPAL ANNIE RAJAN Lisa Wallace Aidan Seeley
title_short	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
title_full	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
title_fullStr	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
title_full_unstemmed	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
title_sort	The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity
author_id_str_mv	977abe5c673627024e4913d034dcbc95 4d91151193de7925ea048bf39f32198b 5e44e9e220512a05e821030192c06160 8904f590a1fafb850f1bb67cf9d682d2 5cebf16bdbc8022118a35da9d13f5087 c69dba86b3ccf9a140b67b7e97d68bba
author_id_fullname_str_mv	977abe5c673627024e4913d034dcbc95_*_Nia Davies 4d91151193de7925ea048bf39f32198b__Julanta Carriere 5e44e9e220512a05e821030192c06160__ANEESHA GOPAL 8904f590a1fafb850f1bb67cf9d682d2__ANNIE RAJAN 5cebf16bdbc8022118a35da9d13f5087__Lisa Wallace c69dba86b3ccf9a140b67b7e97d68bba_*_Aidan Seeley
author	Nia Davies Julanta Carriere ANEESHA GOPAL ANNIE RAJAN Lisa Wallace Aidan Seeley
author2	Nia Davies Julanta Carriere ANEESHA GOPAL ANNIE RAJAN Lisa Wallace Aidan Seeley
format	Journal article
container_title	Pharmacology Biochemistry and Behavior
container_volume	247
container_start_page	173953
publishDate	2025
institution	Swansea University
issn	0091-3057 1873-5177
doi_str_mv	10.1016/j.pbb.2024.173953
publisher	Elsevier BV
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description	Nicotine has been shown to induce profound physiological and behavioural responses in invertebrate model organisms such as Caenorhabditis elegans and Drosophila melanogaster. Lumbriculus variegatus is an aquatic oligochaete worm which we have previously demonstrated has application within pharmacological research. Herein, we demonstrate the presence of endogenous acetylcholine and cholinesterase activity within L. variegatus and show the time-dependent effects on the sensitivity of L. variegatus to nicotine. We describe the effects of a broad range of concentrations of nicotine (1 μM – 1 mM) on L. variegatus response to tactile stimulation and locomotor activity following acute (10-min) and chronic (24-h) exposure. Here, we show that 10 min of exposure to ≥0.1 mM nicotine reversibly reduces the ability of tactile stimulation to elicit stereotypical movements of body reversal and helical swimming, and locomotor activity in L. variegatus. We also demonstrate that exposure to ≥0.1 mM nicotine for 24 h was toxic to L. variegatus. Chronic low-dose nicotine ≥25 μM similarly inhibits L. variegatus behaviours with 50 μM causing irreversible inhibition of movement. Thus, L. variegatus presents a model for studying the effects of nicotine and further demonstrates the application of the in vivo model L. variegatus for behavioural pharmacology research.
published_date	2025-02-01T14:39:20Z
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score	11.048042

The inhibitory effect of nicotine on Lumbriculus variegatus stereotypical movements and locomotor activity

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