No Cover Image

Journal article 6 views

The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations

Michael J. Burgum, Víctor Alcolea-Rodríguez, Hanna Saarelainen, Raquel Portela, Julián J. Reinosa, José F. Fernández, Verónica I. Dumit, Julia Catalán, Felice C. Simeone, Lara Faccani, Martin Clift Orcid Logo, Stephen Evans Orcid Logo, Miguel A. Bañares, Shareen Doak Orcid Logo

NanoImpact, Start page: 100539

Swansea University Authors: Martin Clift Orcid Logo, Stephen Evans Orcid Logo, Shareen Doak Orcid Logo

Full text not available from this repository: check for access using links below.

Check full text

DOI (Published version): 10.1016/j.impact.2024.100539

Abstract

Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they ma...

Full description

Published in: NanoImpact
ISSN: 2452-0748
Published: Elsevier BV 2024
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa68619
first_indexed 2025-01-09T20:33:59Z
last_indexed 2025-01-09T20:33:59Z
id cronfa68619
recordtype SURis
fullrecord <?xml version="1.0"?><rfc1807><datestamp>2024-12-23T10:14:23.3880134</datestamp><bib-version>v2</bib-version><id>68619</id><entry>2024-12-23</entry><title>The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations</title><swanseaauthors><author><sid>71bf49b157691e541950f5c3f49c9169</sid><ORCID>0000-0001-6133-3368</ORCID><firstname>Martin</firstname><surname>Clift</surname><name>Martin Clift</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>cfca981bdfb8492873a48cc1629def9a</sid><ORCID>0000-0002-5352-9800</ORCID><firstname>Stephen</firstname><surname>Evans</surname><name>Stephen Evans</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>8f70286908f67238a527a98cbf66d387</sid><ORCID>0000-0002-6753-1987</ORCID><firstname>Shareen</firstname><surname>Doak</surname><name>Shareen Doak</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2024-12-23</date><deptcode>MEDS</deptcode><abstract>Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o&#x2212; human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24&#x202F;h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o&#x2212; with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.</abstract><type>Journal Article</type><journal>NanoImpact</journal><volume/><journalNumber/><paginationStart>100539</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>2452-0748</issnPrint><issnElectronic/><keywords>CNTs, Oxidative potential, In, vitro genotoxicity, Secondary genotoxicity, NanoGenoTox</keywords><publishedDay>21</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2024</publishedYear><publishedDate>2024-12-21</publishedDate><doi>10.1016/j.impact.2024.100539</doi><url>https://doi.org/10.1016/j.impact.2024.100539</url><notes/><college>COLLEGE NANME</college><department>Medical School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDS</DepartmentCode><institution>Swansea University</institution><apcterm>SU Library paid the OA fee (TA Institutional Deal)</apcterm><funders>Horizon 2020, NanoInformaTIX project 814426</funders><projectreference/><lastEdited>2024-12-23T10:14:23.3880134</lastEdited><Created>2024-12-23T09:59:12.5287383</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Michael J.</firstname><surname>Burgum</surname><order>1</order></author><author><firstname>V&#xED;ctor</firstname><surname>Alcolea-Rodr&#xED;guez</surname><order>2</order></author><author><firstname>Hanna</firstname><surname>Saarelainen</surname><order>3</order></author><author><firstname>Raquel</firstname><surname>Portela</surname><order>4</order></author><author><firstname>Juli&#xE1;n J.</firstname><surname>Reinosa</surname><order>5</order></author><author><firstname>Jos&#xE9; F.</firstname><surname>Fern&#xE1;ndez</surname><order>6</order></author><author><firstname>Ver&#xF3;nica I.</firstname><surname>Dumit</surname><order>7</order></author><author><firstname>Julia</firstname><surname>Catal&#xE1;n</surname><order>8</order></author><author><firstname>Felice C.</firstname><surname>Simeone</surname><order>9</order></author><author><firstname>Lara</firstname><surname>Faccani</surname><order>10</order></author><author><firstname>Martin</firstname><surname>Clift</surname><orcid>0000-0001-6133-3368</orcid><order>11</order></author><author><firstname>Stephen</firstname><surname>Evans</surname><orcid>0000-0002-5352-9800</orcid><order>12</order></author><author><firstname>Miguel A.</firstname><surname>Ba&#xF1;ares</surname><order>13</order></author><author><firstname>Shareen</firstname><surname>Doak</surname><orcid>0000-0002-6753-1987</orcid><order>14</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2024-12-23T10:14:23.3880134 v2 68619 2024-12-23 The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false cfca981bdfb8492873a48cc1629def9a 0000-0002-5352-9800 Stephen Evans Stephen Evans true false 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 2024-12-23 MEDS Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o− human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o− with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms. Journal Article NanoImpact 100539 Elsevier BV 2452-0748 CNTs, Oxidative potential, In, vitro genotoxicity, Secondary genotoxicity, NanoGenoTox 21 12 2024 2024-12-21 10.1016/j.impact.2024.100539 https://doi.org/10.1016/j.impact.2024.100539 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University SU Library paid the OA fee (TA Institutional Deal) Horizon 2020, NanoInformaTIX project 814426 2024-12-23T10:14:23.3880134 2024-12-23T09:59:12.5287383 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Michael J. Burgum 1 Víctor Alcolea-Rodríguez 2 Hanna Saarelainen 3 Raquel Portela 4 Julián J. Reinosa 5 José F. Fernández 6 Verónica I. Dumit 7 Julia Catalán 8 Felice C. Simeone 9 Lara Faccani 10 Martin Clift 0000-0001-6133-3368 11 Stephen Evans 0000-0002-5352-9800 12 Miguel A. Bañares 13 Shareen Doak 0000-0002-6753-1987 14
title The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
spellingShingle The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
Martin Clift
Stephen Evans
Shareen Doak
title_short The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
title_full The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
title_fullStr The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
title_full_unstemmed The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
title_sort The dispersion method does not affect the in vitro genotoxicity of multi-walled carbon nanotubes despite inducing surface alterations
author_id_str_mv 71bf49b157691e541950f5c3f49c9169
cfca981bdfb8492873a48cc1629def9a
8f70286908f67238a527a98cbf66d387
author_id_fullname_str_mv 71bf49b157691e541950f5c3f49c9169_***_Martin Clift
cfca981bdfb8492873a48cc1629def9a_***_Stephen Evans
8f70286908f67238a527a98cbf66d387_***_Shareen Doak
author Martin Clift
Stephen Evans
Shareen Doak
author2 Michael J. Burgum
Víctor Alcolea-Rodríguez
Hanna Saarelainen
Raquel Portela
Julián J. Reinosa
José F. Fernández
Verónica I. Dumit
Julia Catalán
Felice C. Simeone
Lara Faccani
Martin Clift
Stephen Evans
Miguel A. Bañares
Shareen Doak
format Journal article
container_title NanoImpact
container_start_page 100539
publishDate 2024
institution Swansea University
issn 2452-0748
doi_str_mv 10.1016/j.impact.2024.100539
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url https://doi.org/10.1016/j.impact.2024.100539
document_store_str 0
active_str 0
description Multi-walled carbon nanotubes (MWCNTs) are a desirable class of high aspect ratio nanomaterials (HARNs) owing to their extensive applications. Given their demand, the growing occupational and consumer exposure to these materials has warranted an extensive investigation into potential hazards they may pose towards human health. This study utilised both the in vitro mammalian cell gene mutation and the cytokinesis-blocked micronucleus (CBMN) assays to investigate genotoxicity in human lymphoblastoid (TK6) and 16HBE14o− human lung epithelial cells, following exposure to NM-400 and NM-401 MWCNTs for 24 h. To evaluate the potential for secondary genotoxicity, the CBMN assay was applied on a co-culture of 16HBE14o− with differentiated human monocytic (dTHP-1) cells. In addition, two dispersion methods (NanoGenoTox vs. high shear mixing) were utilised prior to exposures and in acellular experiments to assess the effects on MWCNT oxidative potential, aspect ratio and surface properties. These were characterized in chemico as well as by electron microscopy and Raman spectroscopy. Structural damage of NM-400 was observed following both dispersion approaches; Raman spectra highlighted greater oxidative transformation under probe sonication as opposed to high shear mixing. Despite the changes to the oxidative potential of the MWCNTs, no statistically significant genotoxicity was observed under the conditions applied. There was also no visible signs of cellular internaliation of NM-400 or NM-401 into either cell type under the test conditions, which may support the negative genotoxic response. Whilst these HARNs may have oxidative potential, cells have natural protective mechanisms for repairing transient DNA damage. Therefore, it is crucial to evaluate biological endpoints which measure fixed DNA damage to account for the impact of DNA repair mechanisms.
published_date 2024-12-21T08:37:19Z
_version_ 1821393960227045376
score 11.04748

Similar Items