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The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro

Sophie E. Thomas, Joshua Kerry-Smith, Susan F. Plummer, Jack P. Bate, Daniel A. John, Evie Lawrence, Lydia Powell Orcid Logo, Jordanna Dally, Ryan Moseley, Daryn R. Michael

Frontiers in Microbiomes, Volume: 3, Start page: 1488650

Swansea University Author: Lydia Powell Orcid Logo

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DOI (Published version): 10.3389/frmbi.2024.1488650

Abstract

Introduction: Dysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus s...

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Published in: Frontiers in Microbiomes
ISSN: 2813-4338
Published: Frontiers Media SA 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa68318
first_indexed 2024-11-27T19:46:30Z
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spelling 2025-02-13T15:23:18.6070474 v2 68318 2024-11-21 The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro 0e7e702952672bcbfdfd4974199202fb 0000-0002-8641-0160 Lydia Powell Lydia Powell true false 2024-11-21 MEDS Introduction: Dysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus species and two strains of Bifidobacterium species. Methods: Using serum deprived conditions to impair the functionality of immortalized human HaCaT keratinocytes, this study aimed to assess the impact of metabolites derived from the Lab4 probiotic consortium on keratinocyte function. Results: A significant improvement in HaCaT metabolic activity and lower apoptotic activity was observed in tandem with a reduction in Caspase-3 gene expression and a lower Bax/Bcl2 ratio following the addition of Lab4. The probiotic also supported barrier integrity which was better maintained with a significant increase in Filaggrin gene expression. In damaged keratinocytes, Lab4 enhanced rates of re-epithelialization, which were associated with significantly increased gene expression of MMP-1 and enhanced secretion of IL-6 and IL-8. Discussion: These results suggest that the Lab4 probiotic consortium may have the ability to benefit the functionality of skin. Journal Article Frontiers in Microbiomes 3 1488650 Frontiers Media SA 2813-4338 keratinocyte, serum deprivation, probiotic, gut-skin axis, Lab4, conditioned media 19 11 2024 2024-11-19 10.3389/frmbi.2024.1488650 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Other This study was funded by Cultech Ltd. 2025-02-13T15:23:18.6070474 2024-11-21T14:16:38.4592132 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Sophie E. Thomas 1 Joshua Kerry-Smith 2 Susan F. Plummer 3 Jack P. Bate 4 Daniel A. John 5 Evie Lawrence 6 Lydia Powell 0000-0002-8641-0160 7 Jordanna Dally 8 Ryan Moseley 9 Daryn R. Michael 10 68318__33324__c3e4f25e0aaa4aae8467be73e62f13a9.pdf 68318.VoR.pdf 2025-01-14T10:40:16.7221360 Output 996184 application/pdf Version of Record true © 2024 Thomas, Kerry-Smith, Plummer, Bate, John, Lawrence, Powell, Dally, Moseley and Michael. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng http://creativecommons.org/licenses/by/4.0/
title The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
spellingShingle The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
Lydia Powell
title_short The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
title_full The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
title_fullStr The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
title_full_unstemmed The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
title_sort The ability of the Lab4 probiotic consortium to impact upon the functionality of serum deprived human keratinocytes in vitro
author_id_str_mv 0e7e702952672bcbfdfd4974199202fb
author_id_fullname_str_mv 0e7e702952672bcbfdfd4974199202fb_***_Lydia Powell
author Lydia Powell
author2 Sophie E. Thomas
Joshua Kerry-Smith
Susan F. Plummer
Jack P. Bate
Daniel A. John
Evie Lawrence
Lydia Powell
Jordanna Dally
Ryan Moseley
Daryn R. Michael
format Journal article
container_title Frontiers in Microbiomes
container_volume 3
container_start_page 1488650
publishDate 2024
institution Swansea University
issn 2813-4338
doi_str_mv 10.3389/frmbi.2024.1488650
publisher Frontiers Media SA
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
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description Introduction: Dysfunction of keratinocytes contributes to a weakened skin barrier and impaired wound healing capability. Evidence suggests that probiotic supplementation can lead to improved skin function in vitro and in vivo. The Lab4 probiotic consortium comprises of two strains of Lactobacillus species and two strains of Bifidobacterium species. Methods: Using serum deprived conditions to impair the functionality of immortalized human HaCaT keratinocytes, this study aimed to assess the impact of metabolites derived from the Lab4 probiotic consortium on keratinocyte function. Results: A significant improvement in HaCaT metabolic activity and lower apoptotic activity was observed in tandem with a reduction in Caspase-3 gene expression and a lower Bax/Bcl2 ratio following the addition of Lab4. The probiotic also supported barrier integrity which was better maintained with a significant increase in Filaggrin gene expression. In damaged keratinocytes, Lab4 enhanced rates of re-epithelialization, which were associated with significantly increased gene expression of MMP-1 and enhanced secretion of IL-6 and IL-8. Discussion: These results suggest that the Lab4 probiotic consortium may have the ability to benefit the functionality of skin.
published_date 2024-11-19T05:36:20Z
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