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Development and characterisation of a novel complex triple cell culture model of the human alveolar epithelial barrier
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Martin Clift
In vitro models, Volume: 3, Issue: 2-3, Pages: 125 - 137
Swansea University Authors:
Kirsty Meldrum, Josh Bateman, Shareen Doak , Martin Clift
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© The Author(s) 2024. This article is licensed under a Creative Commons Attribution 4.0 International License.
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DOI (Published version): 10.1007/s44164-024-00075-2
Abstract
Owing to increased pressure from ethical groups and the public to avoid unnecessary animal testing, the need for new, responsive and biologically relevant in vitro models has surged. Models of the human alveolar epithelium are of particular interest since thorough investigations into air pollution a...
| Published in: | In vitro models |
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| ISSN: | 2731-3441 |
| Published: |
Springer Science and Business Media LLC
2024
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa67177 |
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| Abstract: |
Owing to increased pressure from ethical groups and the public to avoid unnecessary animal testing, the need for new, responsive and biologically relevant in vitro models has surged. Models of the human alveolar epithelium are of particular interest since thorough investigations into air pollution and the effects of inhaled nanoparticles and e-cigarettes are needed. The lung is a crucial organ of interest due to potential exposures to endogenous material during occupational and ambient settings. Here, an in vitro model of the alveolar barrier has been created in preparation for use in the quasi-air liquid interface (qALI) and (aerosol) air–liquid interface (ALI) exposures. The model consists of an alveolar type 1-like cell line (TT1), an alveolar type 2-like cell line (NCI-H441) and a model of (alveolar) macrophages (dTHP-1). The model formulates a complex, multi-cellular system, cultured at the air–liquid interface, that mimics the apical layer of the alveolar epithelial region in the human lung. Characterisation data has shown that both TT1 and NCI-H441 epithelial cells are able to be cultured together in addition to dTHP-1 cells through imaging (morphology), pro-inflammatory response and viability measurements. This dataset also demonstrates evidence of a reasonable barrier created by the cell culture in comparison to negative controls. Furthermore, it shows that while maintaining a low baseline of (pro)-inflammatory mediator expression during normal conditions, the model is highly responsive to inflammatory stimuli. This model is proposed to be suitable for use in toxicology testing of inhaled exogenous agents. |
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| Keywords: |
In vitro; Multi-cellular system; Epithelial cells; New approach Methodologies (NAMs); Alveolar; 3D cell culture |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
This work has been funded by a combination of scholarships awarded by Knowledge Economy Skills Scholarships (KESS) 2 and UKHSA (UK Health Security Agency). |
| Issue: |
2-3 |
| Start Page: |
125 |
| End Page: |
137 |