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Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes

Norman Ratcliffe

Developmental and Comparative Immunology, Volume: 159, Start page: 105223

Swansea University Author: Norman Ratcliffe

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DOI (Published version): 10.1016/j.dci.2024.105223

Abstract

Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiment...

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Published in: Developmental and Comparative Immunology
ISSN: 0145-305X
Published: Elsevier BV 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa66926
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last_indexed 2024-11-25T14:19:10Z
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spelling 2024-08-01T15:10:08.9571943 v2 66926 2024-07-03 Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes aebb70dc95237305e0a8f4bb80b5ef22 Norman Ratcliffe Norman Ratcliffe true false 2024-07-03 Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting. This article emphasizes that although there are similarities between mammalian neutrophils and arthropod haemocytes, the protocols required for the successful harvesting of these cells vary significantly. The various stages for the successful harvesting of arthropod haemocytes are described in detail and should provide invaluable advice to those requiring both high cell viability and recovery of the different cell types for subsequent experimentation. Journal Article Developmental and Comparative Immunology 159 105223 Elsevier BV 0145-305X Arthropod haemocyte immunity; LPS cell activation; Clotting and cell lysis; Mammalian neutrophils; Galleria and Drosophila models; Limulus and crustaceans clotting; Cell harvesting protocols 1 10 2024 2024-10-01 10.1016/j.dci.2024.105223 COLLEGE NANME COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) Swansea University 2024-08-01T15:10:08.9571943 2024-07-03T09:56:14.5265627 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Norman Ratcliffe 1 66926__31027__41300eed383448c79cf857217b1e9a1d.pdf 66926.VoR.pdf 2024-08-01T15:08:27.5658883 Output 423073 application/pdf Version of Record true Crown Copyright © 2024. This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/
title Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
spellingShingle Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
Norman Ratcliffe
title_short Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
title_full Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
title_fullStr Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
title_full_unstemmed Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
title_sort Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
author_id_str_mv aebb70dc95237305e0a8f4bb80b5ef22
author_id_fullname_str_mv aebb70dc95237305e0a8f4bb80b5ef22_***_Norman Ratcliffe
author Norman Ratcliffe
author2 Norman Ratcliffe
format Journal article
container_title Developmental and Comparative Immunology
container_volume 159
container_start_page 105223
publishDate 2024
institution Swansea University
issn 0145-305X
doi_str_mv 10.1016/j.dci.2024.105223
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting. This article emphasizes that although there are similarities between mammalian neutrophils and arthropod haemocytes, the protocols required for the successful harvesting of these cells vary significantly. The various stages for the successful harvesting of arthropod haemocytes are described in detail and should provide invaluable advice to those requiring both high cell viability and recovery of the different cell types for subsequent experimentation.
published_date 2024-10-01T08:14:17Z
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score 11.0578165

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