Journal article
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes
Norman Ratcliffe
Developmental and Comparative Immunology, Start page: 105223
Swansea University Author: Norman Ratcliffe
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DOI (Published version): 10.1016/j.dci.2024.105223
Abstract
Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiment...
Published in: | Developmental and Comparative Immunology |
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ISSN: | 0145-305X |
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Elsevier BV
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URI: | https://cronfa.swan.ac.uk/Record/cronfa66926 |
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v2 66926 2024-07-03 Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes aebb70dc95237305e0a8f4bb80b5ef22 Norman Ratcliffe Norman Ratcliffe true false 2024-07-03 Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting. This article emphasizes that although there are similarities between mammalian neutrophils and arthropod haemocytes, the protocols required for the successful harvesting of these cells vary significantly. The various stages for the successful harvesting of arthropod haemocytes are described in detail and should provide invaluable advice to those requiring both high cell viability and recovery of the different cell types for subsequent experimentation. Journal Article Developmental and Comparative Immunology 0 105223 Elsevier BV 0145-305X Arthropod haemocyte immunity; LPS cell activation; Clotting and cell lysis; Mammalian neutrophils; Galleria and Drosophila models; Limulus and crustaceans clotting; Cell harvesting protocols 0 0 0 0001-01-01 10.1016/j.dci.2024.105223 COLLEGE NANME COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) Swansea University 2024-07-03T10:05:50.7193127 2024-07-03T09:56:14.5265627 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Norman Ratcliffe 1 |
title |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
spellingShingle |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes Norman Ratcliffe |
title_short |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
title_full |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
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Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
title_full_unstemmed |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
title_sort |
Back to the future: Forgotten protocols for optimizing the isolation of arthropod haemocytes |
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Norman Ratcliffe |
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Norman Ratcliffe |
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Developmental and Comparative Immunology |
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10.1016/j.dci.2024.105223 |
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Elsevier BV |
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Consideration is given to previous and more recent protocols for harvesting arthropod haemocytes from Galleria, Drosophila, mosquitoes, Limulus and crustaceans. The optimal harvesting of these cells is essential for meaningful studies of invertebrate immunity in vitro. The results of such experiments, however, have often been flawed due to a lack of understanding of the fragile nature of arthropod haemocytes on exposure to bacterial lipopolysaccharides, resulting in the aggregation and loss of cell types during haemolymph clotting. This article emphasizes that although there are similarities between mammalian neutrophils and arthropod haemocytes, the protocols required for the successful harvesting of these cells vary significantly. The various stages for the successful harvesting of arthropod haemocytes are described in detail and should provide invaluable advice to those requiring both high cell viability and recovery of the different cell types for subsequent experimentation. |
published_date |
0001-01-01T10:05:50Z |
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11.013148 |