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Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories

Marcos F. Martinez-Moreno Orcid Logo, Cristina Povedano-Priego, Mar Morales-Hidalgo Orcid Logo, Adam Mumford, Elisabet Aranda Orcid Logo, Ramiro Vilchez-Vargas, Fadwa Jroundi, Jesus Ojeda Ledo Orcid Logo, Mohamed L. Merroun

Environmental Pollution, Volume: 358, Start page: 124491

Swansea University Authors: Adam Mumford, Jesus Ojeda Ledo Orcid Logo

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Abstract

The deep geological repository (DGR) concept consists of storing radioactive waste in metal canisters, surrounded by compacted bentonite, and placed deeply into a geological formation. Here, bentonite slurry microcosms with copper canisters, inoculated with bacterial consortium and amended with acet...

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Published in: Environmental Pollution
ISSN: 0269-7491
Published: Elsevier BV 2024
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URI: https://cronfa.swan.ac.uk/Record/cronfa66919
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Here, bentonite slurry microcosms with copper canisters, inoculated with bacterial consortium and amended with acetate, lactate and sulfate were set up to investigate their geochemical evolution over a year under anoxic conditions. The impact of microbial communities on the corrosion of the copper canisters in an early-stage (45 days) was also assessed. The amended bacterial consortium and electron donors/acceptor accelerated the microbial activity, while the heat-shocked process had a retarding effect. The microbial communities partially oxidize lactate to acetate, which is subsequently consumed when the lactate is depleted. Early-stage microbial communities showed that the bacterial consortium reduced microbial diversity with Pseudomonas and Stenotrophomonas dominating the community. However, sulfate-reducing bacteria such as Desulfocurvibacter, Anaerosolibacter, and Desulfosporosinus were enriched coupling oxidation of lactate/acetate with reduction of sulfates. The generated biogenic sulfides, which could mediate the conversion of copper oxides (possibly formed by trapped oxygen molecules on the bentonite or driven by the reduction of H2O) to copper sulfide (Cu2S), were identified by X-ray photoelectron spectroscopy (XPS). 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spelling 2024-09-30T15:17:01.4794294 v2 66919 2024-07-01 Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories 65cb3dadba310d966d2494c03364df52 Adam Mumford Adam Mumford true false 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2024-07-01 The deep geological repository (DGR) concept consists of storing radioactive waste in metal canisters, surrounded by compacted bentonite, and placed deeply into a geological formation. Here, bentonite slurry microcosms with copper canisters, inoculated with bacterial consortium and amended with acetate, lactate and sulfate were set up to investigate their geochemical evolution over a year under anoxic conditions. The impact of microbial communities on the corrosion of the copper canisters in an early-stage (45 days) was also assessed. The amended bacterial consortium and electron donors/acceptor accelerated the microbial activity, while the heat-shocked process had a retarding effect. The microbial communities partially oxidize lactate to acetate, which is subsequently consumed when the lactate is depleted. Early-stage microbial communities showed that the bacterial consortium reduced microbial diversity with Pseudomonas and Stenotrophomonas dominating the community. However, sulfate-reducing bacteria such as Desulfocurvibacter, Anaerosolibacter, and Desulfosporosinus were enriched coupling oxidation of lactate/acetate with reduction of sulfates. The generated biogenic sulfides, which could mediate the conversion of copper oxides (possibly formed by trapped oxygen molecules on the bentonite or driven by the reduction of H2O) to copper sulfide (Cu2S), were identified by X-ray photoelectron spectroscopy (XPS). Overall, these findings shed light on the ideal geochemical conditions that would affect the stability of DGR barriers, emphasizing the impact of the SRB on the corrosion of the metal canisters, the gas generation, and the interaction with components of the bentonite. Journal Article Environmental Pollution 358 124491 Elsevier BV 0269-7491 DGR; Spanish bentonite; Microbial diversity; Electron donors/acceptor; Copper corrosion 1 10 2024 2024-10-01 10.1016/j.envpol.2024.124491 COLLEGE NANME COLLEGE CODE Swansea University Another institution paid the OA fee The present work was supported by the grant RTI2018–101548-B-I00 “ERDF A way of making Europe” to MLM from the “Ministerio de Ciencia, Innovación y Universidades” (Spanish Government). The project leading to this application has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 847593 to MLM. ADM acknowledges funding from the UK Engineering and Physical Sciences Research Council (EPSRC) DTP scholarship (project reference: 2748843). 2024-09-30T15:17:01.4794294 2024-07-01T14:09:59.9077411 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Marcos F. Martinez-Moreno 0000-0002-6808-1922 1 Cristina Povedano-Priego 2 Mar Morales-Hidalgo 0000-0002-4034-6212 3 Adam Mumford 4 Elisabet Aranda 0000-0001-5915-2445 5 Ramiro Vilchez-Vargas 6 Fadwa Jroundi 7 Jesus Ojeda Ledo 0000-0002-2046-1010 8 Mohamed L. Merroun 9 66919__31482__3c3693f3988449b589e4a9e0a743bb3e.pdf 66919.VoR.pdf 2024-09-30T15:14:32.2503870 Output 8401948 application/pdf Version of Record true © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/
title Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
spellingShingle Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
Adam Mumford
Jesus Ojeda Ledo
title_short Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
title_full Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
title_fullStr Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
title_full_unstemmed Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
title_sort Microbial influence in Spanish bentonite slurry microcosms: Unveiling a-year long geochemical evolution and early-stage copper corrosion related to nuclear waste repositories
author_id_str_mv 65cb3dadba310d966d2494c03364df52
4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv 65cb3dadba310d966d2494c03364df52_***_Adam Mumford
4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author Adam Mumford
Jesus Ojeda Ledo
author2 Marcos F. Martinez-Moreno
Cristina Povedano-Priego
Mar Morales-Hidalgo
Adam Mumford
Elisabet Aranda
Ramiro Vilchez-Vargas
Fadwa Jroundi
Jesus Ojeda Ledo
Mohamed L. Merroun
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container_title Environmental Pollution
container_volume 358
container_start_page 124491
publishDate 2024
institution Swansea University
issn 0269-7491
doi_str_mv 10.1016/j.envpol.2024.124491
publisher Elsevier BV
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
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description The deep geological repository (DGR) concept consists of storing radioactive waste in metal canisters, surrounded by compacted bentonite, and placed deeply into a geological formation. Here, bentonite slurry microcosms with copper canisters, inoculated with bacterial consortium and amended with acetate, lactate and sulfate were set up to investigate their geochemical evolution over a year under anoxic conditions. The impact of microbial communities on the corrosion of the copper canisters in an early-stage (45 days) was also assessed. The amended bacterial consortium and electron donors/acceptor accelerated the microbial activity, while the heat-shocked process had a retarding effect. The microbial communities partially oxidize lactate to acetate, which is subsequently consumed when the lactate is depleted. Early-stage microbial communities showed that the bacterial consortium reduced microbial diversity with Pseudomonas and Stenotrophomonas dominating the community. However, sulfate-reducing bacteria such as Desulfocurvibacter, Anaerosolibacter, and Desulfosporosinus were enriched coupling oxidation of lactate/acetate with reduction of sulfates. The generated biogenic sulfides, which could mediate the conversion of copper oxides (possibly formed by trapped oxygen molecules on the bentonite or driven by the reduction of H2O) to copper sulfide (Cu2S), were identified by X-ray photoelectron spectroscopy (XPS). Overall, these findings shed light on the ideal geochemical conditions that would affect the stability of DGR barriers, emphasizing the impact of the SRB on the corrosion of the metal canisters, the gas generation, and the interaction with components of the bentonite.
published_date 2024-10-01T08:21:51Z
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