Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation

Morales-Hidalgo, Mar; Povedano-Priego, Cristina; Martinez-Moreno, Marcos F.; Ledo, Jesus Ojeda; Jroundi, Fadwa; Merroun, Mohamed L.

doi:10.1016/j.jhazmat.2024.135044

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Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation

Mar Morales-Hidalgo, Cristina Povedano-Priego, Marcos F. Martinez-Moreno, Jesus Ojeda Ledo

, Fadwa Jroundi, Mohamed L. Merroun

Journal of Hazardous Materials, Volume: 476, Start page: 135044

Swansea University Author: Jesus Ojeda Ledo

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DOI (Published version): 10.1016/j.jhazmat.2024.135044

Abstract

Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as...

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Published in:	Journal of Hazardous Materials
ISSN:	0304-3894
Published:	Elsevier BV 2024
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa66892

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spelling	2024-10-09T09:41:31.0363551 v2 66892 2024-06-25 Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2024-06-25 EAAS Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as waste leakage (e.g., U) and groundwater infiltration of electron rich donors in the bentonite. After a three-year anaerobic incubation, Illumina sequencing results revealed a bacterial diversity dominated by anaerobic and spore-forming micro-organisms mainly from the phylum Firmicutes. Highly U tolerant and viable bacterial isolates from the genera Peribacillus, Bacillus, and some SRB such as Desulfovibrio and Desulfosporosinus, were enriched from U-amended bentonite. The results obtained by XPS and XRD showed that U was present as U(VI) and as U(IV) species. Regarding U(VI), we have identified biogenic U(VI) phosphates, U(UO2)·(PO4)2, located in the inner part of the bacterial cell membranes in addition to U(VI)-adsorbed to clays such as montmorillonite. Biogenic U(IV) species as uraninite may be produced as result of bacterial enzymatic U(VI) reduction. These findings suggest that under electron donor-rich water-saturation conditions, bentonite microbial community can control U speciation, immobilizing it, and thus enhancing future DGR safety if container rupture and waste leakage occurs. Journal Article Journal of Hazardous Materials 476 135044 Elsevier BV 0304-3894 DGR; bentonite slurry; uranium; long-term incubation, microbial diversity; sulfate reducing bacteria 5 9 2024 2024-09-05 10.1016/j.jhazmat.2024.135044 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee This study was supported by the grant RTI2018–101548-B-I00 “ERDF A way of making Europe” to MLM from the “Ministerio de Ciencia, Innovación y Universidades” (Spanish Government). The project leading to this application has received funding from the European Union's Horizon 2020 research and innovation program under grant agreement No 847593 to MLM and the grant FPU20/00583 to the first author from the “Ministerio de Universidades” (Spanish Government). 2024-10-09T09:41:31.0363551 2024-06-25T16:16:15.4309313 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Mar Morales-Hidalgo 1 Cristina Povedano-Priego 2 Marcos F. Martinez-Moreno 3 Jesus Ojeda Ledo 0000-0002-2046-1010 4 Fadwa Jroundi 5 Mohamed L. Merroun 6 66892__32558__a726b5786b124bdc9c19a566dfa3c575.pdf 66892.VoR.pdf 2024-10-09T09:38:11.6803444 Output 8958192 application/pdf Version of Record true © 2024 The Authors. This is an open access article under the CC BY license. true eng http://creativecommons.org/licenses/by/4.0/
title	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
spellingShingle	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation Jesus Ojeda Ledo
title_short	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
title_full	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
title_fullStr	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
title_full_unstemmed	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
title_sort	Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation
author_id_str_mv	4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv	4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author	Jesus Ojeda Ledo
author2	Mar Morales-Hidalgo Cristina Povedano-Priego Marcos F. Martinez-Moreno Jesus Ojeda Ledo Fadwa Jroundi Mohamed L. Merroun
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publisher	Elsevier BV
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description	Deep geological repositories (DGRs) stand out as one of the optimal options for managing high-level radioactive waste (HLW) such as uranium (U) in the near future. Here, we provide novel insights into microbial behavior in the DGR bentonite barrier, addressing potential worst-case scenarios such as waste leakage (e.g., U) and groundwater infiltration of electron rich donors in the bentonite. After a three-year anaerobic incubation, Illumina sequencing results revealed a bacterial diversity dominated by anaerobic and spore-forming micro-organisms mainly from the phylum Firmicutes. Highly U tolerant and viable bacterial isolates from the genera Peribacillus, Bacillus, and some SRB such as Desulfovibrio and Desulfosporosinus, were enriched from U-amended bentonite. The results obtained by XPS and XRD showed that U was present as U(VI) and as U(IV) species. Regarding U(VI), we have identified biogenic U(VI) phosphates, U(UO2)·(PO4)2, located in the inner part of the bacterial cell membranes in addition to U(VI)-adsorbed to clays such as montmorillonite. Biogenic U(IV) species as uraninite may be produced as result of bacterial enzymatic U(VI) reduction. These findings suggest that under electron donor-rich water-saturation conditions, bentonite microbial community can control U speciation, immobilizing it, and thus enhancing future DGR safety if container rupture and waste leakage occurs.
published_date	2024-09-05T11:56:41Z
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score	11.08895

Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: A mechanistic characterization of U speciation

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