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SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein

Steve Slocombe Orcid Logo, Frédéric Beaudoin, Paul G Donaghy, D.Grahame Hardie, J.Richard Dickinson, Nigel G Halford

Plant Physiology and Biochemistry, Volume: 42, Issue: 2, Pages: 111 - 116

Swansea University Author: Steve Slocombe Orcid Logo

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DOI (Published version): 10.1016/j.plaphy.2003.11.009

Abstract

The nucleotide sequence of cBSnIP2, a cDNA that had been cloned from a barley (Hordeum vulgare) seed endosperm cDNA library by two-hybrid screening with barley SNF1-related protein kinase (SnRKI) was determined. It was found to contain a complete open reading frame encoding a class I heat shock prot...

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Published in: Plant Physiology and Biochemistry
ISSN: 0981-9428
Published: Elsevier BV 2004
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URI: https://cronfa.swan.ac.uk/Record/cronfa65485
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spelling 2024-03-21T16:31:01.1157906 v2 65485 2024-01-22 SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein 4a1ea486a78ed357efdfa053a277ae40 0000-0002-3549-7999 Steve Slocombe Steve Slocombe true false 2024-01-22 BGPS The nucleotide sequence of cBSnIP2, a cDNA that had been cloned from a barley (Hordeum vulgare) seed endosperm cDNA library by two-hybrid screening with barley SNF1-related protein kinase (SnRKI) was determined. It was found to contain a complete open reading frame encoding a class I heat shock protein. Transcripts corresponding to the cDNA (renamed cBHSP17) were detectable in RNA isolated from barley seeds harvested in mid-development but not RNA from roots or leaves. BHSP17 protein was expressed in Escherischia coli and shown to be phosphorylated by SnRKI from barley endosperm and spinach leaf. It was found to be a less effective substrate than 3-hydroxy-3-methylglutaryl-Coenzyme A, a previously identified substrate of SnRK1. However, a specific phosphorylation site at serine-35 was identified by solid phase sequencing of RP-HPLC-purified peptides after phosphorylation by spinach SnRK1. Journal Article Plant Physiology and Biochemistry 42 2 111 116 Elsevier BV 0981-9428 Barley; Endosperm; Hordeum vulgare; Metabolic signalling; Seed; Two-hybrid 1 2 2004 2004-02-01 10.1016/j.plaphy.2003.11.009 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University 2024-03-21T16:31:01.1157906 2024-01-22T14:11:48.8819818 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 0000-0002-3549-7999 1 Frédéric Beaudoin 2 Paul G Donaghy 3 D.Grahame Hardie 4 J.Richard Dickinson 5 Nigel G Halford 6
title SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
spellingShingle SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
Steve Slocombe
title_short SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
title_full SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
title_fullStr SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
title_full_unstemmed SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
title_sort SNF1-related protein kinase (SnRK1) phosphorylates class I heat shock protein
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author Steve Slocombe
author2 Steve Slocombe
Frédéric Beaudoin
Paul G Donaghy
D.Grahame Hardie
J.Richard Dickinson
Nigel G Halford
format Journal article
container_title Plant Physiology and Biochemistry
container_volume 42
container_issue 2
container_start_page 111
publishDate 2004
institution Swansea University
issn 0981-9428
doi_str_mv 10.1016/j.plaphy.2003.11.009
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 0
active_str 0
description The nucleotide sequence of cBSnIP2, a cDNA that had been cloned from a barley (Hordeum vulgare) seed endosperm cDNA library by two-hybrid screening with barley SNF1-related protein kinase (SnRKI) was determined. It was found to contain a complete open reading frame encoding a class I heat shock protein. Transcripts corresponding to the cDNA (renamed cBHSP17) were detectable in RNA isolated from barley seeds harvested in mid-development but not RNA from roots or leaves. BHSP17 protein was expressed in Escherischia coli and shown to be phosphorylated by SnRKI from barley endosperm and spinach leaf. It was found to be a less effective substrate than 3-hydroxy-3-methylglutaryl-Coenzyme A, a previously identified substrate of SnRK1. However, a specific phosphorylation site at serine-35 was identified by solid phase sequencing of RP-HPLC-purified peptides after phosphorylation by spinach SnRK1.
published_date 2004-02-01T08:27:41Z
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score 11.047501

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