Journal article 320 views
Unlocking nature’s treasure-chest: screening for oleaginous algae
Steve Slocombe 
,
QianYi Zhang,
Michael Ross,
Avril Anderson,
Naomi J. Thomas,
Ángela Lapresa,
Cecilia Rad-Menéndez,
Christine N. Campbell,
Kenneth D. Black,
Michele S. Stanley,
John G. Day
,
QianYi Zhang,
Michael Ross,
Avril Anderson,
Naomi J. Thomas,
Ángela Lapresa,
Cecilia Rad-Menéndez,
Christine N. Campbell,
Kenneth D. Black,
Michele S. Stanley,
John G. Day
Scientific Reports, Volume: 5, Issue: 1
Swansea University Author:
Steve Slocombe
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1038/srep09844
Abstract
Micro-algae synthesize high levels of lipids, carbohydrates and proteins photoautotrophically, thus attracting considerable interest for the biotechnological production of fuels, environmental remediation, functional foods and nutraceuticals. Currently, only a few micro-algae species are grown comme...
| Published in: | Scientific Reports |
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| ISSN: | 2045-2322 |
| Published: |
Springer Science and Business Media LLC
2015
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa65472 |
| Abstract: |
Micro-algae synthesize high levels of lipids, carbohydrates and proteins photoautotrophically, thus attracting considerable interest for the biotechnological production of fuels, environmental remediation, functional foods and nutraceuticals. Currently, only a few micro-algae species are grown commercially at large-scale, primarily for "health-foods" and pigments. For a range of potential products (fuel to pharma), high lipid productivity strains are required to mitigate the economic costs of mass culture. Here we present a screen concentrating on marine micro-algal strains, which if suitable for scale-up would minimise competition with agriculture for water. Mass-Spectrophotometric analysis (MS) of nitrogen (N) and carbon (C) was subsequently validated by measurement of total fatty acids (TFA) by Gas-Chromatography (GC). This identified a rapid and accurate screening strategy based on elemental analysis. The screen identified Nannochloropsis oceanica CCAP 849/10 and a marine isolate of Chlorella vulgaris CCAP 211/21A as the best lipid producers. Analysis of C, N, protein, carbohydrate and Fatty Acid (FA) composition identified a suite of strains for further biotechnological applications e.g. Dunaliella polymorpha CCAP 19/14, significantly the most productive for carbohydrates, and Cyclotella cryptica CCAP 1070/2, with utility for EPA production and N-assimilation. |
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| College: |
Faculty of Science and Engineering |
| Issue: |
1 |