Enabling large-scale production of algal oil in continuous output mode

Slocombe, Steve; Huete-Ortega, Maria; Kapoore, Rahul Vijay; Okurowska, Katarzyna; Mair, Alison; Day, John G.; Stanley, Michele S.; Vaidyanathan, Seetharaman

doi:10.1016/j.isci.2021.102743

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Enabling large-scale production of algal oil in continuous output mode

Steve Slocombe

, Maria Huete-Ortega, Rahul Vijay Kapoore, Katarzyna Okurowska, Alison Mair, John G. Day, Michele S. Stanley, Seetharaman Vaidyanathan

iScience, Volume: 24, Issue: 7, Start page: 102743

Swansea University Author: Steve Slocombe

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DOI (Published version): 10.1016/j.isci.2021.102743

Abstract

Large-scale algal oil production requires continuous outputs and a trade-off between growth and oil content. Two unrelated marine algae (Nannochloropsis oceanica [CCAP 849/10] and Chlorella vulgaris [CCAP 211/21A]) that showed high oil production under batch culture were studied under controlled sem...

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Published in:	iScience
ISSN:	2589-0042
Published:	Elsevier BV 2021
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa65455

first_indexed	2024-01-22T12:31:19Z
last_indexed	2024-11-25T14:16:07Z
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spelling	2024-03-21T10:16:16.8033249 v2 65455 2024-01-17 Enabling large-scale production of algal oil in continuous output mode 4a1ea486a78ed357efdfa053a277ae40 0000-0002-3549-7999 Steve Slocombe Steve Slocombe true false 2024-01-17 BGPS Large-scale algal oil production requires continuous outputs and a trade-off between growth and oil content. Two unrelated marine algae (Nannochloropsis oceanica [CCAP 849/10] and Chlorella vulgaris [CCAP 211/21A]) that showed high oil production under batch culture were studied under controlled semicontinuous cultivation conditions. Three essential attributes maximized oil productivity: (i) downregulation of cell size to maximize light absorption under N limitation; (ii) low nutrient-depletion thresholds to trigger oil induction; (iii) a means of carbohydrate suppression in favor of oil. N. oceanica responded better to input N/P variations and is more suited to continuous oil production. A low N/P ratio was effective in both suppressing carbohydrate and reducing cell size concomitant with oil production. In C. vulgaris, nutrient starvation thresholds for oil were higher and carbohydrate was preferentially induced, which impeded stress-level optimization for oil. These differences, which impact continuous oil production at scale, are driven by species adaptation to specific marine habitats. Journal Article iScience 24 7 102743 Elsevier BV 2589-0042 23 7 2021 2021-07-23 10.1016/j.isci.2021.102743 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University We are very grateful to Debra Brennan and Elaine Mitchell for technical assistance at SAMS. All other members of the project consortium, who did not directly participate in this study, are gratefully acknowledged for their indirect contributions. This work is a result of the UKRI BBSRC-DBT funded UK-India SuBB project (BB/K020633/1). S.V. also acknowledges financial support from UKRI EPSRC (EP/E036252/1) to Sheffield. 2024-03-21T10:16:16.8033249 2024-01-17T15:53:19.3729324 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 0000-0002-3549-7999 1 Maria Huete-Ortega 2 Rahul Vijay Kapoore 3 Katarzyna Okurowska 4 Alison Mair 5 John G. Day 6 Michele S. Stanley 7 Seetharaman Vaidyanathan 8 65455__29773__5bf8248666d04ef5ba4eb51f626b035e.pdf 65455_VoR.pdf 2024-03-21T10:14:21.0718468 Output 5001199 application/pdf Version of Record true Copyright: 2021 The Authors. This is an open access article under the CC BY-NC-ND license true eng http://creativecommons.org/licenses/by-nc-nd/4.0/
title	Enabling large-scale production of algal oil in continuous output mode
spellingShingle	Enabling large-scale production of algal oil in continuous output mode Steve Slocombe
title_short	Enabling large-scale production of algal oil in continuous output mode
title_full	Enabling large-scale production of algal oil in continuous output mode
title_fullStr	Enabling large-scale production of algal oil in continuous output mode
title_full_unstemmed	Enabling large-scale production of algal oil in continuous output mode
title_sort	Enabling large-scale production of algal oil in continuous output mode
author_id_str_mv	4a1ea486a78ed357efdfa053a277ae40
author_id_fullname_str_mv	4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
author	Steve Slocombe
author2	Steve Slocombe Maria Huete-Ortega Rahul Vijay Kapoore Katarzyna Okurowska Alison Mair John G. Day Michele S. Stanley Seetharaman Vaidyanathan
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container_title	iScience
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publisher	Elsevier BV
college_str	Faculty of Science and Engineering
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description	Large-scale algal oil production requires continuous outputs and a trade-off between growth and oil content. Two unrelated marine algae (Nannochloropsis oceanica [CCAP 849/10] and Chlorella vulgaris [CCAP 211/21A]) that showed high oil production under batch culture were studied under controlled semicontinuous cultivation conditions. Three essential attributes maximized oil productivity: (i) downregulation of cell size to maximize light absorption under N limitation; (ii) low nutrient-depletion thresholds to trigger oil induction; (iii) a means of carbohydrate suppression in favor of oil. N. oceanica responded better to input N/P variations and is more suited to continuous oil production. A low N/P ratio was effective in both suppressing carbohydrate and reducing cell size concomitant with oil production. In C. vulgaris, nutrient starvation thresholds for oil were higher and carbohydrate was preferentially induced, which impeded stress-level optimization for oil. These differences, which impact continuous oil production at scale, are driven by species adaptation to specific marine habitats.
published_date	2021-07-23T05:32:04Z
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Enabling large-scale production of algal oil in continuous output mode

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