In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis

THOMAS, NATHAN; Tang, Kam; Coates, Christopher J.

doi:10.1007/s13199-023-00964-2

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In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis

NATHAN THOMAS, Kam Tang

, Christopher J. Coates

Symbiosis, Volume: 92, Issue: 1, Pages: 137 - 148

Swansea University Authors: NATHAN THOMAS, Kam Tang

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DOI (Published version): 10.1007/s13199-023-00964-2

Abstract

Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coa...

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Published in:	Symbiosis
ISSN:	0334-5114 1878-7665
Published:	Springer Science and Business Media LLC 2024
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URI:	https://cronfa.swan.ac.uk/Record/cronfa65247
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spelling	v2 65247 2023-12-07 In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis ea301a73007049cf191d0caf171a3b8c NATHAN THOMAS NATHAN THOMAS true false 69af43a3b9da24aef65c5d3a44956fe3 0000-0001-9427-9564 Kam Tang Kam Tang true false 2023-12-07 Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coast, from Wales to Portugal. The only known population of the worm in the United Kingdom was discovered in Wales decades ago, but very little research has been conducted since. For 13 months, we measured how environmental conditions such as temperature, salinity and light intensity coincided with population size at the Welsh field site. To establish phylogenetic relationships among the different populations and their algal symbionts, we designed new polymerase chain reaction (PCR) oligonucleotides to assess the nucleotide diversity of the mitochondrial cytochrome c oxidase I subunit (COI) gene in gDNA extracted from representative worms across their known range (Wales, France, Portugal, Spain, and Guernsey). We also targeted the 18S rRNA gene of their algal symbiont, Tetraselmis convolutae. We observed temporal shifts in environmental factors coinciding with fluctuating worm colony size, notably temperature. Based on the molecular data, the worm exhibited different ecotypes across locations, while the algal symbiont showed little genetic variation. Journal Article Symbiosis 92 1 137 148 Springer Science and Business Media LLC 0334-5114 1878-7665 1 1 2024 2024-01-01 10.1007/s13199-023-00964-2 http://dx.doi.org/10.1007/s13199-023-00964-2 COLLEGE NANME COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) HEFCW. We would like to thank everyone who kindly provided samples from the various field locations: Mel Broadhurst-Allen (Alderney wildlife trust, Guernsey), Luis Oliveira (Portuguese Institute for Sea and Atmosphere-IMPA, Portugal), and Xavier Bailly (Station Biologique De Roscoff, France). We are grateful to Dr Jessica Bevan (née Thomas) for the technical support. 2024-04-04T16:15:53.6143099 2023-12-07T16:57:56.9046100 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences NATHAN THOMAS 1 Kam Tang 0000-0001-9427-9564 2 Christopher J. Coates 3 65247__29246__a99122aac78340239c7effb2dbcd9dd9.pdf Symbiosis_online.pdf 2023-12-12T09:26:18.4357234 Output 3196509 application/pdf Version of Record true Distributed using a Creative Commons Attribution CC-BY 4.0 licence. true eng http://creativecommons.org/licenses/by/4.0/
title	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
spellingShingle	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis NATHAN THOMAS Kam Tang
title_short	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
title_full	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
title_fullStr	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
title_full_unstemmed	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
title_sort	In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis
author_id_str_mv	ea301a73007049cf191d0caf171a3b8c 69af43a3b9da24aef65c5d3a44956fe3
author_id_fullname_str_mv	ea301a73007049cf191d0caf171a3b8c_*_NATHAN THOMAS 69af43a3b9da24aef65c5d3a44956fe3_*_Kam Tang
author	NATHAN THOMAS Kam Tang
author2	NATHAN THOMAS Kam Tang Christopher J. Coates
format	Journal article
container_title	Symbiosis
container_volume	92
container_issue	1
container_start_page	137
publishDate	2024
institution	Swansea University
issn	0334-5114 1878-7665
doi_str_mv	10.1007/s13199-023-00964-2
publisher	Springer Science and Business Media LLC
college_str	Faculty of Science and Engineering
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hierarchy_top_title	Faculty of Science and Engineering
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department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url	http://dx.doi.org/10.1007/s13199-023-00964-2
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description	Known as the Roscoff worm or mint-sauce worm, Symsagittifera roscoffensis is an Acoel distinguishable due to the presence of symbiotic alga Tetraselmis convolutae, held beneath the epidermis. Isolated populations of S. roscoffensis span a broad geographical range along the north-eastern Atlantic coast, from Wales to Portugal. The only known population of the worm in the United Kingdom was discovered in Wales decades ago, but very little research has been conducted since. For 13 months, we measured how environmental conditions such as temperature, salinity and light intensity coincided with population size at the Welsh field site. To establish phylogenetic relationships among the different populations and their algal symbionts, we designed new polymerase chain reaction (PCR) oligonucleotides to assess the nucleotide diversity of the mitochondrial cytochrome c oxidase I subunit (COI) gene in gDNA extracted from representative worms across their known range (Wales, France, Portugal, Spain, and Guernsey). We also targeted the 18S rRNA gene of their algal symbiont, Tetraselmis convolutae. We observed temporal shifts in environmental factors coinciding with fluctuating worm colony size, notably temperature. Based on the molecular data, the worm exhibited different ecotypes across locations, while the algal symbiont showed little genetic variation.
published_date	2024-01-01T16:15:49Z
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In situ environmental conditions and molecular identification of the photosymbiotic marine worm Symsagittifera roscoffensis

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