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Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer / SUSAN CHANDLER

Swansea University Author: SUSAN CHANDLER

  • E-Thesis under embargo until: 23rd September 2026

DOI (Published version): 10.23889/SUthesis.65058

Abstract

Colorectal cancer is one of the most common cancers worldwide. There is a need for a biomarker with the ability to accurately detect disease status during treatment or follow up. Raman Spectroscopy is a technique using vibrational spectroscopy with the ability to accurately detect components such as...

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Published: Swansea, Wales, UK 2023
Institution: Swansea University
Degree level: Doctoral
Degree name: M.D
Supervisor: Jenkins, Gareth J., Dunstan Peter R. and Harris, Dean A.
URI: https://cronfa.swan.ac.uk/Record/cronfa65058
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first_indexed 2023-11-21T12:02:30Z
last_indexed 2023-11-21T12:02:30Z
id cronfa65058
recordtype RisThesis
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spelling v2 65058 2023-11-21 Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer 9def2ae9b1f40b1f10a808aabe389267 SUSAN CHANDLER SUSAN CHANDLER true false 2023-11-21 Colorectal cancer is one of the most common cancers worldwide. There is a need for a biomarker with the ability to accurately detect disease status during treatment or follow up. Raman Spectroscopy is a technique using vibrational spectroscopy with the ability to accurately detect components such as carbohydrates, proteins, nucleic acids, lipids and more in blood serum. This thesis is focused on the investigation of Raman Spectroscopy as a technique for disease monitoring during treatment of colorectal cancer. Serum samples of patients undergoing surgical resection of colorectal cancer in the perioperative period, and patients undergoing chemotherapy treatment for colorectal cancer were analysed looking for changes during treatment related to disease. Changes in the serum were detected throughout the perioperative period, in regions associated with guanine, phenylalanine and tryptophan. Phenylalanine and tryptophan have previously been used in the diagnosis of colorectal cancer using Raman spectroscopy. Changes seen in regions associated with DNA and nucleotide bases may be linked to circulating tumour DNA during the perioperative period. Changes were seen in areas of the spectra associated with fatty acids and amino acids, that correlate with changes in metabolism related to the hallmarks of cancer. In patients undergoing chemotherapy, changes were noted in patients with disease recurrence prior to detection of recurrent disease by current methods. The serum spectra of patients with recurrence became more variable in the later treatment cycles than non-recurrence patients. Testing of the diagnostic RAMAN-CRC model showed potential for a machine learning model to predict disease status, with data from this study providing information to commence model building. This thesis concludes by discussing the clinical application of Raman spectroscopy and the potential role as a disease monitoring tool during treatment. As a more accurate and efficient tool than current techniques, serum Raman spectroscopy could have significant impact on patient outcomes. E-Thesis Swansea, Wales, UK Colorectal cancer, Raman spectroscopy, biomarker 21 2 2023 2023-02-21 10.23889/SUthesis.65058 COLLEGE NANME COLLEGE CODE Swansea University Jenkins, Gareth J., Dunstan Peter R. and Harris, Dean A. Doctoral M.D Swansea University Swansea University 2023-11-21T12:08:45.2284880 2023-11-21T11:59:12.2293201 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science SUSAN CHANDLER 1 Under embargo Under embargo 2023-11-21T12:07:50.9349259 Output 8453610 application/pdf E-Thesis true 2026-09-23T00:00:00.0000000 Copyright: The Author, Susan Chandler, 2023. true eng
title Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
spellingShingle Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
SUSAN CHANDLER
title_short Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
title_full Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
title_fullStr Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
title_full_unstemmed Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
title_sort Serum Raman Spectroscopy as a disease monitoring tool during treatment for colorectal cancer
author_id_str_mv 9def2ae9b1f40b1f10a808aabe389267
author_id_fullname_str_mv 9def2ae9b1f40b1f10a808aabe389267_***_SUSAN CHANDLER
author SUSAN CHANDLER
author2 SUSAN CHANDLER
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publishDate 2023
institution Swansea University
doi_str_mv 10.23889/SUthesis.65058
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
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description Colorectal cancer is one of the most common cancers worldwide. There is a need for a biomarker with the ability to accurately detect disease status during treatment or follow up. Raman Spectroscopy is a technique using vibrational spectroscopy with the ability to accurately detect components such as carbohydrates, proteins, nucleic acids, lipids and more in blood serum. This thesis is focused on the investigation of Raman Spectroscopy as a technique for disease monitoring during treatment of colorectal cancer. Serum samples of patients undergoing surgical resection of colorectal cancer in the perioperative period, and patients undergoing chemotherapy treatment for colorectal cancer were analysed looking for changes during treatment related to disease. Changes in the serum were detected throughout the perioperative period, in regions associated with guanine, phenylalanine and tryptophan. Phenylalanine and tryptophan have previously been used in the diagnosis of colorectal cancer using Raman spectroscopy. Changes seen in regions associated with DNA and nucleotide bases may be linked to circulating tumour DNA during the perioperative period. Changes were seen in areas of the spectra associated with fatty acids and amino acids, that correlate with changes in metabolism related to the hallmarks of cancer. In patients undergoing chemotherapy, changes were noted in patients with disease recurrence prior to detection of recurrent disease by current methods. The serum spectra of patients with recurrence became more variable in the later treatment cycles than non-recurrence patients. Testing of the diagnostic RAMAN-CRC model showed potential for a machine learning model to predict disease status, with data from this study providing information to commence model building. This thesis concludes by discussing the clinical application of Raman spectroscopy and the potential role as a disease monitoring tool during treatment. As a more accurate and efficient tool than current techniques, serum Raman spectroscopy could have significant impact on patient outcomes.
published_date 2023-02-21T12:08:45Z
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score 11.013148

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