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Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow

Bregje W. M. de Wildt Orcid Logo, Feihu Zhao Orcid Logo, Iris Lauwers, Bert van Rietbergen, Keita Ito, Sandra Hofmann Orcid Logo

Biotechnology and Bioengineering, Volume: 120, Issue: 7, Pages: 2013 - 2026

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.1002/bit.28418

Abstract

The transition in the field of bone tissue engineering from bone regeneration to in vitro models has come with the challenge of recreating a dense and anisotropic bone-like extracellular matrix (ECM). Although the mechanism by which bone ECM gains its structure is not fully understood, mechanical lo...

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Published in: Biotechnology and Bioengineering
ISSN: 0006-3592 1097-0290
Published: Wiley 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64828
first_indexed 2023-10-26T10:44:15Z
last_indexed 2024-11-25T14:14:49Z
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spelling 2023-11-15T10:11:51.2710224 v2 64828 2023-10-26 Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2023-10-26 EAAS The transition in the field of bone tissue engineering from bone regeneration to in vitro models has come with the challenge of recreating a dense and anisotropic bone-like extracellular matrix (ECM). Although the mechanism by which bone ECM gains its structure is not fully understood, mechanical loading and curvature have been identified as potential contributors. Here, guided by computational simulations, we evaluated cell and bone-like tissue growth and organization in a concave channel with and without directional fluid flow stimulation. Human mesenchymal stromal cells were seeded on donut-shaped silk fibroin scaffolds and osteogenically stimulated for 42 days statically or in a flow perfusion bioreactor. After 14, 28, and 42 days, constructs were investigated for cell and tissue growth and organization. As a result, directional fluid flow was able to improve organic tissue growth but not organization. Cells tended to orient in the tangential direction of the channel, possibly attributed to its curvature. Based on our results, we suggest that organic ECM production but not anisotropy can be stimulated through the application of fluid flow. With this study, an initial attempt in three-dimensions was made to improve the resemblance of in vitro produced bone-like ECM to the physiological bone ECM. Journal Article Biotechnology and Bioengineering 120 7 2013 2026 Wiley 0006-3592 1097-0290 Bone tissue engineering, cell and tissue organization, computational fluid dynamics model, extracellular matrix, in vitro model 31 7 2023 2023-07-31 10.1002/bit.28418 http://dx.doi.org/10.1002/bit.28418 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee NWO-TTW (TTW 016.Vidi.188.021) 2023-11-15T10:11:51.2710224 2023-10-26T11:40:51.2008636 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Bregje W. M. de Wildt 0000-0001-7502-8794 1 Feihu Zhao 0000-0003-0515-6808 2 Iris Lauwers 3 Bert van Rietbergen 4 Keita Ito 5 Sandra Hofmann 0000-0002-2568-8388 6 64828__28879__2bd44d0722104ce0b84af15bba620611.pdf 64828.pdf 2023-10-26T11:43:38.4554382 Output 6961315 application/pdf Version of Record true © 2023 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC. Distributed under the terms of a Creative Commons Attribution 4.0 International License (CC BY 4.0). true eng http://creativecommons.org/licenses/by/4.0/
title Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
spellingShingle Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
Feihu Zhao
title_short Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
title_full Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
title_fullStr Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
title_full_unstemmed Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
title_sort Characterization of three‐dimensional bone‐like tissue growth and organization under influence of directional fluid flow
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Bregje W. M. de Wildt
Feihu Zhao
Iris Lauwers
Bert van Rietbergen
Keita Ito
Sandra Hofmann
format Journal article
container_title Biotechnology and Bioengineering
container_volume 120
container_issue 7
container_start_page 2013
publishDate 2023
institution Swansea University
issn 0006-3592
1097-0290
doi_str_mv 10.1002/bit.28418
publisher Wiley
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
url http://dx.doi.org/10.1002/bit.28418
document_store_str 1
active_str 0
description The transition in the field of bone tissue engineering from bone regeneration to in vitro models has come with the challenge of recreating a dense and anisotropic bone-like extracellular matrix (ECM). Although the mechanism by which bone ECM gains its structure is not fully understood, mechanical loading and curvature have been identified as potential contributors. Here, guided by computational simulations, we evaluated cell and bone-like tissue growth and organization in a concave channel with and without directional fluid flow stimulation. Human mesenchymal stromal cells were seeded on donut-shaped silk fibroin scaffolds and osteogenically stimulated for 42 days statically or in a flow perfusion bioreactor. After 14, 28, and 42 days, constructs were investigated for cell and tissue growth and organization. As a result, directional fluid flow was able to improve organic tissue growth but not organization. Cells tended to orient in the tangential direction of the channel, possibly attributed to its curvature. Based on our results, we suggest that organic ECM production but not anisotropy can be stimulated through the application of fluid flow. With this study, an initial attempt in three-dimensions was made to improve the resemblance of in vitro produced bone-like ECM to the physiological bone ECM.
published_date 2023-07-31T14:34:49Z
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