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One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors

F. Mastrotto, A. F. Breen, G. Sicilia, S. Murdan, A. D. Johnstone, Georgina Marsh, C. Grainger-Boultby, N. A. Russell, C. Alexander Orcid Logo, G. Mantovani

Polymer Chemistry, Volume: 7, Issue: 44, Pages: 6714 - 6724

Swansea University Author: Georgina Marsh

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DOI (Published version): 10.1039/c6py01292b

Abstract

Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Usin...

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Published in: Polymer Chemistry
ISSN: 1759-9954 1759-9962
Published: Royal Society of Chemistry (RSC) 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa64802
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spelling 2023-11-15T14:29:41.8237061 v2 64802 2023-10-23 One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors 60eb67dc80ac6072cbea39caa88d662c Georgina Marsh Georgina Marsh true false 2023-10-23 ONDF Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Using Perrier's protocol, well-defined amphiphilic diblock copolymers formed from PEG acrylate (mPEGA480), 2-(acryloyloxy)ethyl-3-chloro-4-hydroxybenzoate (ACH) or 2-(3-chloro-4-hydroxybenzamido)ethyl acrylate (CHB), have been synthesised by RAFT polymerisation in one-pot, pushing the monomer conversion for each block close to completion (≥94%). The reaction mixture, consisting of green biocompatible solvents (ethanol/water) have then been directly utilised to generate well-defined polymersomes, by simple cannulation into water or in a more automated process, by using a bespoke microfluidic device. Terbinafine and cyanocobalamine were used to demonstrate the suitability of the process to incorporate model hydrophobic and hydrophilic drugs, respectively. Vesicles size and morphology were characterised by DLS, TEM, and AFM. In this work we show that materials and experimental conditions can be chosen to allow facile and rapid generation drug-loaded polymersomes, through a suitable in-line process, directly from acrylate or acrylamide monomer building blocks. Journal Article Polymer Chemistry 7 44 6714 6724 Royal Society of Chemistry (RSC) 1759-9954 1759-9962 Polymersomes, monomers 20 9 2016 2016-09-20 10.1039/c6py01292b http://dx.doi.org/10.1039/c6py01292b COLLEGE NANME Other/Subsidiary Companies - Not Defined COLLEGE CODE ONDF Swansea University The authors thank the UK Engineering and Physical Sciences Research Council (EPSRC: Grants EP/H006915/1 and EP/H005625/1 Leadership Fellowship (C. A.); EP/L01646X (A. F. B.); EP/I01375X/1 (G. E. M.)). 2023-11-15T14:29:41.8237061 2023-10-23T14:58:38.9174721 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Pharmacy F. Mastrotto 1 A. F. Breen 2 G. Sicilia 3 S. Murdan 4 A. D. Johnstone 5 Georgina Marsh 6 C. Grainger-Boultby 7 N. A. Russell 8 C. Alexander 0000-0001-8337-1875 9 G. Mantovani 10 64802__29032__2b2db73a09a6405d904b476ec3752b58.pdf 64802.VOR.pdf 2023-11-15T14:17:12.7484522 Output 3414221 application/pdf Version of Record true Distributed under the terms of a Creative Commons Attribution 3.0 Unported License (CC BY 3.0). true eng https://creativecommons.org/licenses/by/3.0/
title One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
spellingShingle One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
Georgina Marsh
title_short One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
title_full One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
title_fullStr One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
title_full_unstemmed One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
title_sort One-pot RAFT and fast polymersomes assembly: a ‘beeline’ from monomers to drug-loaded nanovectors
author_id_str_mv 60eb67dc80ac6072cbea39caa88d662c
author_id_fullname_str_mv 60eb67dc80ac6072cbea39caa88d662c_***_Georgina Marsh
author Georgina Marsh
author2 F. Mastrotto
A. F. Breen
G. Sicilia
S. Murdan
A. D. Johnstone
Georgina Marsh
C. Grainger-Boultby
N. A. Russell
C. Alexander
G. Mantovani
format Journal article
container_title Polymer Chemistry
container_volume 7
container_issue 44
container_start_page 6714
publishDate 2016
institution Swansea University
issn 1759-9954
1759-9962
doi_str_mv 10.1039/c6py01292b
publisher Royal Society of Chemistry (RSC)
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Pharmacy{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Pharmacy
url http://dx.doi.org/10.1039/c6py01292b
document_store_str 1
active_str 0
description Rapid and simple routes to functional polymersomes are increasingly needed to expand their clinical or industrial applications. Here we describe a novel strategy where polymersomes are prepared through an in-line process in just a few hours, starting from simple acrylate or acrylamide monomers. Using Perrier's protocol, well-defined amphiphilic diblock copolymers formed from PEG acrylate (mPEGA480), 2-(acryloyloxy)ethyl-3-chloro-4-hydroxybenzoate (ACH) or 2-(3-chloro-4-hydroxybenzamido)ethyl acrylate (CHB), have been synthesised by RAFT polymerisation in one-pot, pushing the monomer conversion for each block close to completion (≥94%). The reaction mixture, consisting of green biocompatible solvents (ethanol/water) have then been directly utilised to generate well-defined polymersomes, by simple cannulation into water or in a more automated process, by using a bespoke microfluidic device. Terbinafine and cyanocobalamine were used to demonstrate the suitability of the process to incorporate model hydrophobic and hydrophilic drugs, respectively. Vesicles size and morphology were characterised by DLS, TEM, and AFM. In this work we show that materials and experimental conditions can be chosen to allow facile and rapid generation drug-loaded polymersomes, through a suitable in-line process, directly from acrylate or acrylamide monomer building blocks.
published_date 2016-09-20T18:19:45Z
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score 11.059359

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