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Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

Clotilde Théry Orcid Logo, Kenneth W Witwer Orcid Logo, Elena Aikawa, Maria Jose Alcaraz, Johnathon D Anderson, Ramaroson Andriantsitohaina, Anna Antoniou, Tanina Arab, Fabienne Archer, Georgia K Atkin‐Smith, D Craig Ayre, Jean‐Marie Bach, Daniel Bachurski, Hossein Baharvand, Leonora Balaj, Shawn Baldacchino, Natalie N Bauer, Amy A Baxter, Mary Bebawy, Carla Beckham, Apolonija Bedina Zavec, Abderrahim Benmoussa, Anna C Berardi, Paolo Bergese, Ewa Bielska, Cherie Blenkiron, Sylwia Bobis‐Wozowicz, Eric Boilard, Wilfrid Boireau, Antonella Bongiovanni, Francesc E Borràs, Steffi Bosch, Chantal M Boulanger, Xandra Breakefield, Andrew M Breglio, Meadhbh Á Brennan, David R Brigstock, Alain Brisson, Marike LD Broekman, Jacqueline F Bromberg, Paulina Bryl‐Górecka, Shilpa Buch, Amy H Buck, Dylan Burger, Sara Busatto, Dominik Buschmann, Benedetta Bussolati, Edit I Buzás, James Bryan Byrd, Giovanni Camussi, David RF Carter, Sarah Caruso, Lawrence W Chamley, Yu‐Ting Chang, Chihchen Chen, Shuai Chen, Lesley Cheng, Andrew R Chin, Aled Clayton, Stefano P Clerici, Alex Cocks, Emanuele Cocucci, Robert J Coffey, Anabela Cordeiro‐da‐Silva, Yvonne Couch, Frank AW Coumans, Beth Coyle, Rossella Crescitelli, Miria Ferreira Criado, Crislyn D'Souza‐Schorey, Saumya Das, Amrita Datta Chaudhuri, Paola de Candia, Eliezer F De Santana, Olivier De Wever, Hernando A del Portillo, Tanguy Demaret, Sarah Deville, Andrew Devitt, Bert Dhondt, Dolores Di Vizio, Lothar C Dieterich, Vincenza Dolo, Ana Paula Dominguez Rubio, Massimo Dominici, Mauricio R Dourado, Tom AP Driedonks, Filipe V Duarte, Heather M Duncan, Ramon M Eichenberger, Karin Ekström, Samir EL Andaloussi, Celine Elie‐Caille, Uta Erdbrügger, Juan M Falcón‐Pérez, Farah Fatima, Jason E Fish, Miguel Flores‐Bellver, András Försönits, Annie Frelet‐Barrand, Fabia Fricke, Gregor Fuhrmann, Susanne Gabrielsson, Ana Gámez‐Valero, Chris Gardiner, Kathrin Gärtner, Raphael Gaudin, Yong Song Gho, Bernd Giebel, Caroline Gilbert, Mario Gimona, Ilaria Giusti, Deborah CI Goberdhan, André Görgens, Sharon M Gorski, David W Greening, Julia Christina Gross, Alice Gualerzi, Gopal N Gupta, Dakota Gustafson, Aase Handberg, Reka A Haraszti, Paul Harrison, Hargita Hegyesi, An Hendrix, Andrew F Hill, Fred H Hochberg, Karl F Hoffmann, Beth Holder, Harry Holthofer, Baharak Hosseinkhani, Guoku Hu, Yiyao Huang, Veronica Huber, Stuart Hunt, Ahmed Gamal‐Eldin Ibrahim, Tsuneya Ikezu, Jameel M Inal, Mustafa Isin, Alena Ivanova, Hannah K Jackson, Soren Jacobsen, Steven M Jay, Muthuvel Jayachandran, Guido Jenster, Lanzhou Jiang, Suzanne M Johnson, Jennifer C Jones, Ambrose Jong, Tijana Jovanovic‐Talisman, Stephanie Jung, Raghu Kalluri, Shin‐ichi Kano, Sukhbir Kaur, Yumi Kawamura, Evan T Keller, Delaram Khamari, Elena Khomyakova, Anastasia Khvorova, Peter Kierulf, Kwang Pyo Kim, Thomas Kislinger, Mikael Klingeborn, David J Klinke, Miroslaw Kornek, Maja M Kosanović, Árpád Ferenc Kovács, Eva‐Maria Krämer‐Albers, Susanne Krasemann, Mirja Krause, Igor V Kurochkin, Gina D Kusuma, Sören Kuypers, Saara Laitinen, Scott M Langevin, Lucia R Languino, Joanne Lannigan, Cecilia Lässer, Louise C Laurent, Gregory Lavieu, Elisa Lázaro‐Ibáñez, Soazig Le Lay, Myung‐Shin Lee, Yi Xin Fiona Lee, Debora S Lemos, Metka Lenassi, Aleksandra Leszczynska, Isaac TS Li, Ke Liao, Sten F Libregts, Erzsebet Ligeti, Rebecca Lim, Sai Kiang Lim, Aija Linē, Karen Linnemannstöns, Alicia Llorente, Catherine A Lombard, Magdalena J Lorenowicz, Ákos M Lörincz, Jan Lötvall, Jason Lovett, Michelle C Lowry, Xavier Loyer, Quan Lu, Barbara Lukomska, Taral R Lunavat, Sybren LN Maas, Harmeet Malhi, Antonio Marcilla, Jacopo Mariani, Javier Mariscal, Elena S Martens‐Uzunova, Lorena Martin‐Jaular, M Carmen Martinez, Vilma Regina Martins, Mathilde Mathieu, Suresh Mathivanan, Marco Maugeri, Lynda K McGinnis, Mark J McVey, David G Meckes, Katie L Meehan, Inge Mertens, Valentina R Minciacchi, Andreas Möller, Malene Møller Jørgensen, Aizea Morales‐Kastresana, Jess Morhayim, François Mullier, Maurizio Muraca, Luca Musante, Veronika Mussack, Dillon C Muth, Kathryn H Myburgh, Tanbir Najrana, Muhammad Nawaz, Irina Nazarenko, Peter Nejsum, Christian Neri, Tommaso Neri, Rienk Nieuwland, Leonardo Nimrichter, John P Nolan, Esther NM Nolte‐'t Hoen, Nicole Noren Hooten, Lorraine O'Driscoll, Tina O'Grady, Ana O'Loghlen, Takahiro Ochiya, Martin Olivier, Alberto Ortiz, Luis A Ortiz, Xabier Osteikoetxea, Ole Østergaard, Matias Ostrowski, Jaesung Park, D. Michiel Pegtel, Hector Peinado, Francesca Perut, Michael W Pfaffl, Donald G Phinney, Bartijn CH Pieters, Ryan C Pink, David S Pisetsky, Elke Pogge von Strandmann, Iva Polakovicova, Ivan KH Poon, Bonita H Powell, Ilaria Prada, Lynn Pulliam, Peter Quesenberry, Annalisa Radeghieri, Robert L Raffai, Stefania Raimondo, Janusz Rak, Marcel I Ramirez, Graça Raposo, Morsi S Rayyan, Neta Regev‐Rudzki, Franz L Ricklefs, Paul D Robbins, David D Roberts, Silvia C Rodrigues, Eva Rohde, Sophie Rome, Kasper MA Rouschop, Aurelia Rughetti, Ashley E Russell, Paula Saá, Susmita Sahoo, Edison Salas‐Huenuleo, Catherine Sánchez, Julie A Saugstad, Meike J Saul, Raymond M Schiffelers, Raphael Schneider, Tine Hiorth Schøyen, Aaron Scott, Eriomina Shahaj, Shivani Sharma, Olga Shatnyeva, Faezeh Shekari, Ganesh Vilas Shelke, Ashok K Shetty, Kiyotaka Shiba, Pia R‐M Siljander, Andreia M Silva, Agata Skowronek, Orman L Snyder, Rodrigo Pedro Soares, Barbara W Sódar, Carolina Soekmadji, Javier Sotillo, Philip D Stahl, Willem Stoorvogel, Shannon L Stott, Erwin F Strasser, Simon Swift, Hidetoshi Tahara, Muneesh Tewari, Kate Timms, Swasti Tiwari, Rochelle Tixeira, Mercedes Tkach, Wei Seong Toh, Richard Tomasini, Ana Claudia Torrecilhas, Juan Pablo Tosar, Vasilis Toxavidis, Lorena Urbanelli, Pieter Vader, Bas WM van Balkom, Susanne G van der Grein, Jan Van Deun, Martijn JC van Herwijnen, Kendall Van Keuren‐Jensen, Guillaume van Niel, Martin E van Royen, Andre J van Wijnen, M Helena Vasconcelos, Ivan J Vechetti, Tiago D Veit, Laura J Vella, Émilie Velot, Frederik J Verweij, Beate Vestad, Jose L Viñas, Tamás Visnovitz, Krisztina V Vukman, Jessica Wahlgren, Dionysios C Watson, Marca HM Wauben, Alissa Weaver, Jason Webber Orcid Logo, Viktoria Weber, Ann M Wehman, Daniel J Weiss, Joshua A Welsh, Sebastian Wendt, Asa M Wheelock, Zoltán Wiener, Leonie Witte, Joy Wolfram, Angeliki Xagorari, Patricia Xander, Jing Xu, Xiaomei Yan, María Yáñez‐Mó, Hang Yin, Yuana Yuana, Valentina Zappulli, Jana Zarubova, Vytautas Žėkas, Jian‐ye Zhang, Zezhou Zhao, Lei Zheng, Alexander R Zheutlin, Antje M Zickler, Pascale Zimmermann, Angela M Zivkovic, Davide Zocco, Ewa K Zuba‐Surma

Journal of Extracellular Vesicles, Volume: 7, Issue: 1

Swansea University Author: Jason Webber Orcid Logo

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    © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. Distributed under the terms of a Creative Commons Attribution Non Commercial 4.0 License (CC BY-NC 4.0).

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DOI (Published version): 10.1080/20013078.2018.1535750

Abstract

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles,...

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Published in: Journal of Extracellular Vesicles
ISSN: 2001-3078 2001-3078
Published: Wiley 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa64722
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fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>64722</id><entry>2023-10-11</entry><title>Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines</title><swanseaauthors><author><sid>25d1a26f9b8bb556bd9412080e40351d</sid><ORCID>0000-0003-4772-3014</ORCID><firstname>Jason</firstname><surname>Webber</surname><name>Jason Webber</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2023-10-11</date><deptcode>BMS</deptcode><abstract>The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.</abstract><type>Journal Article</type><journal>Journal of Extracellular Vesicles</journal><volume>7</volume><journalNumber>1</journalNumber><paginationStart/><paginationEnd/><publisher>Wiley</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>2001-3078</issnPrint><issnElectronic>2001-3078</issnElectronic><keywords>Extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-12-31</publishedDate><doi>10.1080/20013078.2018.1535750</doi><url>http://dx.doi.org/10.1080/20013078.2018.1535750</url><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm>Another institution paid the OA fee</apcterm><funders>Funding is listed only for the corresponding authors. CT’s laboratory is funded by INSERM, Institut Curie, Ministry of Education, and grants from INCa (INCA-11548), French National Research Agency (ANR-10-IDEX-0001-02 PSL* and ANR-11-LABX-0043), SIDACTION (17-1-AAE-1138), Fondation ARC (PGA1 RF20180206962, PJA 20171206453). KWW and CT receive support from NIDA (DA040385). KWW is also supported in part by NIA AG057430, NIDA DA047807 and NIMH MH118164.</funders><projectreference/><lastEdited>2023-11-28T11:21:13.1097758</lastEdited><Created>2023-10-11T16:33:10.5994088</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Biomedical Science</level></path><authors><author><firstname>Clotilde</firstname><surname>Théry</surname><orcid>0000-0001-8294-6884</orcid><order>1</order></author><author><firstname>Kenneth W</firstname><surname>Witwer</surname><orcid>0000-0003-1664-4233</orcid><order>2</order></author><author><firstname>Elena</firstname><surname>Aikawa</surname><order>3</order></author><author><firstname>Maria Jose</firstname><surname>Alcaraz</surname><order>4</order></author><author><firstname>Johnathon D</firstname><surname>Anderson</surname><order>5</order></author><author><firstname>Ramaroson</firstname><surname>Andriantsitohaina</surname><order>6</order></author><author><firstname>Anna</firstname><surname>Antoniou</surname><order>7</order></author><author><firstname>Tanina</firstname><surname>Arab</surname><order>8</order></author><author><firstname>Fabienne</firstname><surname>Archer</surname><order>9</order></author><author><firstname>Georgia K</firstname><surname>Atkin‐Smith</surname><order>10</order></author><author><firstname>D 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spelling v2 64722 2023-10-11 Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines 25d1a26f9b8bb556bd9412080e40351d 0000-0003-4772-3014 Jason Webber Jason Webber true false 2023-10-11 BMS The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points. Journal Article Journal of Extracellular Vesicles 7 1 Wiley 2001-3078 2001-3078 Extracellular vesicles, exosomes, ectosomes, microvesicles, minimal information requirements, guidelines, standardization, microparticles, rigor, reproducibility 31 12 2018 2018-12-31 10.1080/20013078.2018.1535750 http://dx.doi.org/10.1080/20013078.2018.1535750 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Another institution paid the OA fee Funding is listed only for the corresponding authors. CT’s laboratory is funded by INSERM, Institut Curie, Ministry of Education, and grants from INCa (INCA-11548), French National Research Agency (ANR-10-IDEX-0001-02 PSL* and ANR-11-LABX-0043), SIDACTION (17-1-AAE-1138), Fondation ARC (PGA1 RF20180206962, PJA 20171206453). KWW and CT receive support from NIDA (DA040385). KWW is also supported in part by NIA AG057430, NIDA DA047807 and NIMH MH118164. 2023-11-28T11:21:13.1097758 2023-10-11T16:33:10.5994088 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Clotilde Théry 0000-0001-8294-6884 1 Kenneth W Witwer 0000-0003-1664-4233 2 Elena Aikawa 3 Maria Jose Alcaraz 4 Johnathon D Anderson 5 Ramaroson Andriantsitohaina 6 Anna Antoniou 7 Tanina Arab 8 Fabienne Archer 9 Georgia K Atkin‐Smith 10 D Craig Ayre 11 Jean‐Marie Bach 12 Daniel Bachurski 13 Hossein Baharvand 14 Leonora Balaj 15 Shawn Baldacchino 16 Natalie N Bauer 17 Amy A Baxter 18 Mary Bebawy 19 Carla Beckham 20 Apolonija Bedina Zavec 21 Abderrahim Benmoussa 22 Anna C Berardi 23 Paolo Bergese 24 Ewa Bielska 25 Cherie Blenkiron 26 Sylwia Bobis‐Wozowicz 27 Eric Boilard 28 Wilfrid Boireau 29 Antonella Bongiovanni 30 Francesc E Borràs 31 Steffi Bosch 32 Chantal M Boulanger 33 Xandra Breakefield 34 Andrew M Breglio 35 Meadhbh Á Brennan 36 David R Brigstock 37 Alain Brisson 38 Marike LD Broekman 39 Jacqueline F Bromberg 40 Paulina Bryl‐Górecka 41 Shilpa Buch 42 Amy H Buck 43 Dylan Burger 44 Sara Busatto 45 Dominik Buschmann 46 Benedetta Bussolati 47 Edit I Buzás 48 James Bryan Byrd 49 Giovanni Camussi 50 David RF Carter 51 Sarah Caruso 52 Lawrence W Chamley 53 Yu‐Ting Chang 54 Chihchen Chen 55 Shuai Chen 56 Lesley Cheng 57 Andrew R Chin 58 Aled Clayton 59 Stefano P Clerici 60 Alex Cocks 61 Emanuele Cocucci 62 Robert J Coffey 63 Anabela Cordeiro‐da‐Silva 64 Yvonne Couch 65 Frank AW Coumans 66 Beth Coyle 67 Rossella Crescitelli 68 Miria Ferreira Criado 69 Crislyn D'Souza‐Schorey 70 Saumya Das 71 Amrita Datta Chaudhuri 72 Paola de Candia 73 Eliezer F De Santana 74 Olivier De Wever 75 Hernando A del Portillo 76 Tanguy Demaret 77 Sarah Deville 78 Andrew Devitt 79 Bert Dhondt 80 Dolores Di Vizio 81 Lothar C Dieterich 82 Vincenza Dolo 83 Ana Paula Dominguez Rubio 84 Massimo Dominici 85 Mauricio R Dourado 86 Tom AP Driedonks 87 Filipe V Duarte 88 Heather M Duncan 89 Ramon M Eichenberger 90 Karin Ekström 91 Samir EL Andaloussi 92 Celine Elie‐Caille 93 Uta Erdbrügger 94 Juan M Falcón‐Pérez 95 Farah Fatima 96 Jason E Fish 97 Miguel Flores‐Bellver 98 András Försönits 99 Annie Frelet‐Barrand 100 Fabia Fricke 101 Gregor Fuhrmann 102 Susanne Gabrielsson 103 Ana Gámez‐Valero 104 Chris Gardiner 105 Kathrin Gärtner 106 Raphael Gaudin 107 Yong Song Gho 108 Bernd Giebel 109 Caroline Gilbert 110 Mario Gimona 111 Ilaria Giusti 112 Deborah CI Goberdhan 113 André Görgens 114 Sharon M Gorski 115 David W Greening 116 Julia Christina Gross 117 Alice Gualerzi 118 Gopal N Gupta 119 Dakota Gustafson 120 Aase Handberg 121 Reka A Haraszti 122 Paul Harrison 123 Hargita Hegyesi 124 An Hendrix 125 Andrew F Hill 126 Fred H Hochberg 127 Karl F Hoffmann 128 Beth Holder 129 Harry Holthofer 130 Baharak Hosseinkhani 131 Guoku Hu 132 Yiyao Huang 133 Veronica Huber 134 Stuart Hunt 135 Ahmed Gamal‐Eldin Ibrahim 136 Tsuneya Ikezu 137 Jameel M Inal 138 Mustafa Isin 139 Alena Ivanova 140 Hannah K Jackson 141 Soren Jacobsen 142 Steven M Jay 143 Muthuvel Jayachandran 144 Guido Jenster 145 Lanzhou Jiang 146 Suzanne M Johnson 147 Jennifer C Jones 148 Ambrose Jong 149 Tijana Jovanovic‐Talisman 150 Stephanie Jung 151 Raghu Kalluri 152 Shin‐ichi Kano 153 Sukhbir Kaur 154 Yumi Kawamura 155 Evan T Keller 156 Delaram Khamari 157 Elena Khomyakova 158 Anastasia Khvorova 159 Peter Kierulf 160 Kwang Pyo Kim 161 Thomas Kislinger 162 Mikael Klingeborn 163 David J Klinke 164 Miroslaw Kornek 165 Maja M Kosanović 166 Árpád Ferenc Kovács 167 Eva‐Maria Krämer‐Albers 168 Susanne Krasemann 169 Mirja Krause 170 Igor V Kurochkin 171 Gina D Kusuma 172 Sören Kuypers 173 Saara Laitinen 174 Scott M Langevin 175 Lucia R Languino 176 Joanne Lannigan 177 Cecilia Lässer 178 Louise C Laurent 179 Gregory Lavieu 180 Elisa Lázaro‐Ibáñez 181 Soazig Le Lay 182 Myung‐Shin Lee 183 Yi Xin Fiona Lee 184 Debora S Lemos 185 Metka Lenassi 186 Aleksandra Leszczynska 187 Isaac TS Li 188 Ke Liao 189 Sten F Libregts 190 Erzsebet Ligeti 191 Rebecca Lim 192 Sai Kiang Lim 193 Aija Linē 194 Karen Linnemannstöns 195 Alicia Llorente 196 Catherine A Lombard 197 Magdalena J Lorenowicz 198 Ákos M Lörincz 199 Jan Lötvall 200 Jason Lovett 201 Michelle C Lowry 202 Xavier Loyer 203 Quan Lu 204 Barbara Lukomska 205 Taral R Lunavat 206 Sybren LN Maas 207 Harmeet Malhi 208 Antonio Marcilla 209 Jacopo Mariani 210 Javier Mariscal 211 Elena S Martens‐Uzunova 212 Lorena Martin‐Jaular 213 M Carmen Martinez 214 Vilma Regina Martins 215 Mathilde Mathieu 216 Suresh Mathivanan 217 Marco Maugeri 218 Lynda K McGinnis 219 Mark J McVey 220 David G Meckes 221 Katie L Meehan 222 Inge Mertens 223 Valentina R Minciacchi 224 Andreas Möller 225 Malene Møller Jørgensen 226 Aizea Morales‐Kastresana 227 Jess Morhayim 228 François Mullier 229 Maurizio Muraca 230 Luca Musante 231 Veronika Mussack 232 Dillon C Muth 233 Kathryn H Myburgh 234 Tanbir Najrana 235 Muhammad Nawaz 236 Irina Nazarenko 237 Peter Nejsum 238 Christian Neri 239 Tommaso Neri 240 Rienk Nieuwland 241 Leonardo Nimrichter 242 John P Nolan 243 Esther NM Nolte‐'t Hoen 244 Nicole Noren Hooten 245 Lorraine O'Driscoll 246 Tina O'Grady 247 Ana O'Loghlen 248 Takahiro Ochiya 249 Martin Olivier 250 Alberto Ortiz 251 Luis A Ortiz 252 Xabier Osteikoetxea 253 Ole Østergaard 254 Matias Ostrowski 255 Jaesung Park 256 D. Michiel Pegtel 257 Hector Peinado 258 Francesca Perut 259 Michael W Pfaffl 260 Donald G Phinney 261 Bartijn CH Pieters 262 Ryan C Pink 263 David S Pisetsky 264 Elke Pogge von Strandmann 265 Iva Polakovicova 266 Ivan KH Poon 267 Bonita H Powell 268 Ilaria Prada 269 Lynn Pulliam 270 Peter Quesenberry 271 Annalisa Radeghieri 272 Robert L Raffai 273 Stefania Raimondo 274 Janusz Rak 275 Marcel I Ramirez 276 Graça Raposo 277 Morsi S Rayyan 278 Neta Regev‐Rudzki 279 Franz L Ricklefs 280 Paul D Robbins 281 David D Roberts 282 Silvia C Rodrigues 283 Eva Rohde 284 Sophie Rome 285 Kasper MA Rouschop 286 Aurelia Rughetti 287 Ashley E Russell 288 Paula Saá 289 Susmita Sahoo 290 Edison Salas‐Huenuleo 291 Catherine Sánchez 292 Julie A Saugstad 293 Meike J Saul 294 Raymond M Schiffelers 295 Raphael Schneider 296 Tine Hiorth Schøyen 297 Aaron Scott 298 Eriomina Shahaj 299 Shivani Sharma 300 Olga Shatnyeva 301 Faezeh Shekari 302 Ganesh Vilas Shelke 303 Ashok K Shetty 304 Kiyotaka Shiba 305 Pia R‐M Siljander 306 Andreia M Silva 307 Agata Skowronek 308 Orman L Snyder 309 Rodrigo Pedro Soares 310 Barbara W Sódar 311 Carolina Soekmadji 312 Javier Sotillo 313 Philip D Stahl 314 Willem Stoorvogel 315 Shannon L Stott 316 Erwin F Strasser 317 Simon Swift 318 Hidetoshi Tahara 319 Muneesh Tewari 320 Kate Timms 321 Swasti Tiwari 322 Rochelle Tixeira 323 Mercedes Tkach 324 Wei Seong Toh 325 Richard Tomasini 326 Ana Claudia Torrecilhas 327 Juan Pablo Tosar 328 Vasilis Toxavidis 329 Lorena Urbanelli 330 Pieter Vader 331 Bas WM van Balkom 332 Susanne G van der Grein 333 Jan Van Deun 334 Martijn JC van Herwijnen 335 Kendall Van Keuren‐Jensen 336 Guillaume van Niel 337 Martin E van Royen 338 Andre J van Wijnen 339 M Helena Vasconcelos 340 Ivan J Vechetti 341 Tiago D Veit 342 Laura J Vella 343 Émilie Velot 344 Frederik J Verweij 345 Beate Vestad 346 Jose L Viñas 347 Tamás Visnovitz 348 Krisztina V Vukman 349 Jessica Wahlgren 350 Dionysios C Watson 351 Marca HM Wauben 352 Alissa Weaver 353 Jason Webber 0000-0003-4772-3014 354 Viktoria Weber 355 Ann M Wehman 356 Daniel J Weiss 357 Joshua A Welsh 358 Sebastian Wendt 359 Asa M Wheelock 360 Zoltán Wiener 361 Leonie Witte 362 Joy Wolfram 363 Angeliki Xagorari 364 Patricia Xander 365 Jing Xu 366 Xiaomei Yan 367 María Yáñez‐Mó 368 Hang Yin 369 Yuana Yuana 370 Valentina Zappulli 371 Jana Zarubova 372 Vytautas Žėkas 373 Jian‐ye Zhang 374 Zezhou Zhao 375 Lei Zheng 376 Alexander R Zheutlin 377 Antje M Zickler 378 Pascale Zimmermann 379 Angela M Zivkovic 380 Davide Zocco 381 Ewa K Zuba‐Surma 382 64722__29131__5f7d7ebe4d3d41ccb40df1693c3f8fd8.pdf 64722.VOR.pdf 2023-11-28T11:19:17.1687673 Output 4067469 application/pdf Version of Record true © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles. Distributed under the terms of a Creative Commons Attribution Non Commercial 4.0 License (CC BY-NC 4.0). true eng https://creativecommons.org/licenses/by-nc/4.0/
title Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
spellingShingle Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Jason Webber
title_short Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
title_full Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
title_fullStr Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
title_full_unstemmed Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
title_sort Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
author_id_str_mv 25d1a26f9b8bb556bd9412080e40351d
author_id_fullname_str_mv 25d1a26f9b8bb556bd9412080e40351d_***_Jason Webber
author Jason Webber
author2 Clotilde Théry
Kenneth W Witwer
Elena Aikawa
Maria Jose Alcaraz
Johnathon D Anderson
Ramaroson Andriantsitohaina
Anna Antoniou
Tanina Arab
Fabienne Archer
Georgia K Atkin‐Smith
D Craig Ayre
Jean‐Marie Bach
Daniel Bachurski
Hossein Baharvand
Leonora Balaj
Shawn Baldacchino
Natalie N Bauer
Amy A Baxter
Mary Bebawy
Carla Beckham
Apolonija Bedina Zavec
Abderrahim Benmoussa
Anna C Berardi
Paolo Bergese
Ewa Bielska
Cherie Blenkiron
Sylwia Bobis‐Wozowicz
Eric Boilard
Wilfrid Boireau
Antonella Bongiovanni
Francesc E Borràs
Steffi Bosch
Chantal M Boulanger
Xandra Breakefield
Andrew M Breglio
Meadhbh Á Brennan
David R Brigstock
Alain Brisson
Marike LD Broekman
Jacqueline F Bromberg
Paulina Bryl‐Górecka
Shilpa Buch
Amy H Buck
Dylan Burger
Sara Busatto
Dominik Buschmann
Benedetta Bussolati
Edit I Buzás
James Bryan Byrd
Giovanni Camussi
David RF Carter
Sarah Caruso
Lawrence W Chamley
Yu‐Ting Chang
Chihchen Chen
Shuai Chen
Lesley Cheng
Andrew R Chin
Aled Clayton
Stefano P Clerici
Alex Cocks
Emanuele Cocucci
Robert J Coffey
Anabela Cordeiro‐da‐Silva
Yvonne Couch
Frank AW Coumans
Beth Coyle
Rossella Crescitelli
Miria Ferreira Criado
Crislyn D'Souza‐Schorey
Saumya Das
Amrita Datta Chaudhuri
Paola de Candia
Eliezer F De Santana
Olivier De Wever
Hernando A del Portillo
Tanguy Demaret
Sarah Deville
Andrew Devitt
Bert Dhondt
Dolores Di Vizio
Lothar C Dieterich
Vincenza Dolo
Ana Paula Dominguez Rubio
Massimo Dominici
Mauricio R Dourado
Tom AP Driedonks
Filipe V Duarte
Heather M Duncan
Ramon M Eichenberger
Karin Ekström
Samir EL Andaloussi
Celine Elie‐Caille
Uta Erdbrügger
Juan M Falcón‐Pérez
Farah Fatima
Jason E Fish
Miguel Flores‐Bellver
András Försönits
Annie Frelet‐Barrand
Fabia Fricke
Gregor Fuhrmann
Susanne Gabrielsson
Ana Gámez‐Valero
Chris Gardiner
Kathrin Gärtner
Raphael Gaudin
Yong Song Gho
Bernd Giebel
Caroline Gilbert
Mario Gimona
Ilaria Giusti
Deborah CI Goberdhan
André Görgens
Sharon M Gorski
David W Greening
Julia Christina Gross
Alice Gualerzi
Gopal N Gupta
Dakota Gustafson
Aase Handberg
Reka A Haraszti
Paul Harrison
Hargita Hegyesi
An Hendrix
Andrew F Hill
Fred H Hochberg
Karl F Hoffmann
Beth Holder
Harry Holthofer
Baharak Hosseinkhani
Guoku Hu
Yiyao Huang
Veronica Huber
Stuart Hunt
Ahmed Gamal‐Eldin Ibrahim
Tsuneya Ikezu
Jameel M Inal
Mustafa Isin
Alena Ivanova
Hannah K Jackson
Soren Jacobsen
Steven M Jay
Muthuvel Jayachandran
Guido Jenster
Lanzhou Jiang
Suzanne M Johnson
Jennifer C Jones
Ambrose Jong
Tijana Jovanovic‐Talisman
Stephanie Jung
Raghu Kalluri
Shin‐ichi Kano
Sukhbir Kaur
Yumi Kawamura
Evan T Keller
Delaram Khamari
Elena Khomyakova
Anastasia Khvorova
Peter Kierulf
Kwang Pyo Kim
Thomas Kislinger
Mikael Klingeborn
David J Klinke
Miroslaw Kornek
Maja M Kosanović
Árpád Ferenc Kovács
Eva‐Maria Krämer‐Albers
Susanne Krasemann
Mirja Krause
Igor V Kurochkin
Gina D Kusuma
Sören Kuypers
Saara Laitinen
Scott M Langevin
Lucia R Languino
Joanne Lannigan
Cecilia Lässer
Louise C Laurent
Gregory Lavieu
Elisa Lázaro‐Ibáñez
Soazig Le Lay
Myung‐Shin Lee
Yi Xin Fiona Lee
Debora S Lemos
Metka Lenassi
Aleksandra Leszczynska
Isaac TS Li
Ke Liao
Sten F Libregts
Erzsebet Ligeti
Rebecca Lim
Sai Kiang Lim
Aija Linē
Karen Linnemannstöns
Alicia Llorente
Catherine A Lombard
Magdalena J Lorenowicz
Ákos M Lörincz
Jan Lötvall
Jason Lovett
Michelle C Lowry
Xavier Loyer
Quan Lu
Barbara Lukomska
Taral R Lunavat
Sybren LN Maas
Harmeet Malhi
Antonio Marcilla
Jacopo Mariani
Javier Mariscal
Elena S Martens‐Uzunova
Lorena Martin‐Jaular
M Carmen Martinez
Vilma Regina Martins
Mathilde Mathieu
Suresh Mathivanan
Marco Maugeri
Lynda K McGinnis
Mark J McVey
David G Meckes
Katie L Meehan
Inge Mertens
Valentina R Minciacchi
Andreas Möller
Malene Møller Jørgensen
Aizea Morales‐Kastresana
Jess Morhayim
François Mullier
Maurizio Muraca
Luca Musante
Veronika Mussack
Dillon C Muth
Kathryn H Myburgh
Tanbir Najrana
Muhammad Nawaz
Irina Nazarenko
Peter Nejsum
Christian Neri
Tommaso Neri
Rienk Nieuwland
Leonardo Nimrichter
John P Nolan
Esther NM Nolte‐'t Hoen
Nicole Noren Hooten
Lorraine O'Driscoll
Tina O'Grady
Ana O'Loghlen
Takahiro Ochiya
Martin Olivier
Alberto Ortiz
Luis A Ortiz
Xabier Osteikoetxea
Ole Østergaard
Matias Ostrowski
Jaesung Park
D. Michiel Pegtel
Hector Peinado
Francesca Perut
Michael W Pfaffl
Donald G Phinney
Bartijn CH Pieters
Ryan C Pink
David S Pisetsky
Elke Pogge von Strandmann
Iva Polakovicova
Ivan KH Poon
Bonita H Powell
Ilaria Prada
Lynn Pulliam
Peter Quesenberry
Annalisa Radeghieri
Robert L Raffai
Stefania Raimondo
Janusz Rak
Marcel I Ramirez
Graça Raposo
Morsi S Rayyan
Neta Regev‐Rudzki
Franz L Ricklefs
Paul D Robbins
David D Roberts
Silvia C Rodrigues
Eva Rohde
Sophie Rome
Kasper MA Rouschop
Aurelia Rughetti
Ashley E Russell
Paula Saá
Susmita Sahoo
Edison Salas‐Huenuleo
Catherine Sánchez
Julie A Saugstad
Meike J Saul
Raymond M Schiffelers
Raphael Schneider
Tine Hiorth Schøyen
Aaron Scott
Eriomina Shahaj
Shivani Sharma
Olga Shatnyeva
Faezeh Shekari
Ganesh Vilas Shelke
Ashok K Shetty
Kiyotaka Shiba
Pia R‐M Siljander
Andreia M Silva
Agata Skowronek
Orman L Snyder
Rodrigo Pedro Soares
Barbara W Sódar
Carolina Soekmadji
Javier Sotillo
Philip D Stahl
Willem Stoorvogel
Shannon L Stott
Erwin F Strasser
Simon Swift
Hidetoshi Tahara
Muneesh Tewari
Kate Timms
Swasti Tiwari
Rochelle Tixeira
Mercedes Tkach
Wei Seong Toh
Richard Tomasini
Ana Claudia Torrecilhas
Juan Pablo Tosar
Vasilis Toxavidis
Lorena Urbanelli
Pieter Vader
Bas WM van Balkom
Susanne G van der Grein
Jan Van Deun
Martijn JC van Herwijnen
Kendall Van Keuren‐Jensen
Guillaume van Niel
Martin E van Royen
Andre J van Wijnen
M Helena Vasconcelos
Ivan J Vechetti
Tiago D Veit
Laura J Vella
Émilie Velot
Frederik J Verweij
Beate Vestad
Jose L Viñas
Tamás Visnovitz
Krisztina V Vukman
Jessica Wahlgren
Dionysios C Watson
Marca HM Wauben
Alissa Weaver
Jason Webber
Viktoria Weber
Ann M Wehman
Daniel J Weiss
Joshua A Welsh
Sebastian Wendt
Asa M Wheelock
Zoltán Wiener
Leonie Witte
Joy Wolfram
Angeliki Xagorari
Patricia Xander
Jing Xu
Xiaomei Yan
María Yáñez‐Mó
Hang Yin
Yuana Yuana
Valentina Zappulli
Jana Zarubova
Vytautas Žėkas
Jian‐ye Zhang
Zezhou Zhao
Lei Zheng
Alexander R Zheutlin
Antje M Zickler
Pascale Zimmermann
Angela M Zivkovic
Davide Zocco
Ewa K Zuba‐Surma
format Journal article
container_title Journal of Extracellular Vesicles
container_volume 7
container_issue 1
publishDate 2018
institution Swansea University
issn 2001-3078
2001-3078
doi_str_mv 10.1080/20013078.2018.1535750
publisher Wiley
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.1080/20013078.2018.1535750
document_store_str 1
active_str 0
description The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
published_date 2018-12-31T11:21:15Z
_version_ 1783806532878598144
score 11.013148

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