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Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

Uta Erdbrügger, Charles J. Blijdorp, Irene V. Bijnsdorp, Francesc E. Borràs, Dylan Burger, Benedetta Bussolati, James Brian Byrd, Aled Clayton, James W. Dear, Juan M. Falcón‐Pérez, Cristina Grange, Andrew F. Hill, Harry Holthöfer, Ewout J. Hoorn, Guido Jenster, Connie R. Jimenez, Kerstin Junker, John Klein, Mark A. Knepper, Erik H. Koritzinsky, James M. Luther, Metka Lenassi, Janne Leivo, Inge Mertens, Luca Musante, Eline Oeyen, Maija Puhka, Martin E. van Royen, Catherine Sánchez, Carolina Soekmadji, Visith Thongboonkerd, Volkert van Steijn, Gerald Verhaegh, Jason Webber Orcid Logo, Kenneth Witwer, Peter S.T. Yuen, Lei Zheng, Alicia Llorente, Elena S. Martens‐Uzunova

Journal of Extracellular Vesicles, Volume: 10, Issue: 7

Swansea University Author: Jason Webber Orcid Logo

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DOI (Published version): 10.1002/jev2.12093

Abstract

Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and path...

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Published in: Journal of Extracellular Vesicles
ISSN: 2001-3078
Published: Wiley 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa64711
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spelling v2 64711 2023-10-11 Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles 25d1a26f9b8bb556bd9412080e40351d 0000-0003-4772-3014 Jason Webber Jason Webber true false 2023-10-11 BMS Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field. Journal Article Journal of Extracellular Vesicles 10 7 Wiley 2001-3078 Biobank, biomarkers, bladder, extracellular vesicles, kidney, liquid biopsy, prostate, rigor and standardization, urine 31 5 2021 2021-05-31 10.1002/jev2.12093 http://dx.doi.org/10.1002/jev2.12093 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Another institution paid the OA fee ESM-U, CG, GV, GJ, IVB, MvR, and VvS, are members of the “IMMPROVE” consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015-8022). AL is supported by Norges Forskningsråd, Kreftforeningen and Helse Sør-Øst RHF (NO). UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23-HL-126101. CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05. 2023-11-27T16:29:08.2678016 2023-10-11T11:54:30.7774834 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Uta Erdbrügger 1 Charles J. Blijdorp 2 Irene V. Bijnsdorp 3 Francesc E. Borràs 4 Dylan Burger 5 Benedetta Bussolati 6 James Brian Byrd 7 Aled Clayton 8 James W. Dear 9 Juan M. Falcón‐Pérez 10 Cristina Grange 11 Andrew F. Hill 12 Harry Holthöfer 13 Ewout J. Hoorn 14 Guido Jenster 15 Connie R. Jimenez 16 Kerstin Junker 17 John Klein 18 Mark A. Knepper 19 Erik H. Koritzinsky 20 James M. Luther 21 Metka Lenassi 22 Janne Leivo 23 Inge Mertens 24 Luca Musante 25 Eline Oeyen 26 Maija Puhka 27 Martin E. van Royen 28 Catherine Sánchez 29 Carolina Soekmadji 30 Visith Thongboonkerd 31 Volkert van Steijn 32 Gerald Verhaegh 33 Jason Webber 0000-0003-4772-3014 34 Kenneth Witwer 35 Peter S.T. Yuen 36 Lei Zheng 37 Alicia Llorente 38 Elena S. Martens‐Uzunova 39 64711__29125__583c76c5b6d141a99e4eb7b29a4f6d0c.pdf 64711.VOR.pdf 2023-11-27T16:26:26.6157247 Output 2828141 application/pdf Version of Record true © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. Distributed under the terms of a Creative Commons Attribution 4.0 International License (CC BY 4.0). true eng https://creativecommons.org/licenses/by/4.0/
title Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
spellingShingle Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
Jason Webber
title_short Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_full Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_fullStr Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_full_unstemmed Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
title_sort Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles
author_id_str_mv 25d1a26f9b8bb556bd9412080e40351d
author_id_fullname_str_mv 25d1a26f9b8bb556bd9412080e40351d_***_Jason Webber
author Jason Webber
author2 Uta Erdbrügger
Charles J. Blijdorp
Irene V. Bijnsdorp
Francesc E. Borràs
Dylan Burger
Benedetta Bussolati
James Brian Byrd
Aled Clayton
James W. Dear
Juan M. Falcón‐Pérez
Cristina Grange
Andrew F. Hill
Harry Holthöfer
Ewout J. Hoorn
Guido Jenster
Connie R. Jimenez
Kerstin Junker
John Klein
Mark A. Knepper
Erik H. Koritzinsky
James M. Luther
Metka Lenassi
Janne Leivo
Inge Mertens
Luca Musante
Eline Oeyen
Maija Puhka
Martin E. van Royen
Catherine Sánchez
Carolina Soekmadji
Visith Thongboonkerd
Volkert van Steijn
Gerald Verhaegh
Jason Webber
Kenneth Witwer
Peter S.T. Yuen
Lei Zheng
Alicia Llorente
Elena S. Martens‐Uzunova
format Journal article
container_title Journal of Extracellular Vesicles
container_volume 10
container_issue 7
publishDate 2021
institution Swansea University
issn 2001-3078
doi_str_mv 10.1002/jev2.12093
publisher Wiley
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.1002/jev2.12093
document_store_str 1
active_str 0
description Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field.
published_date 2021-05-31T16:29:08Z
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score 11.037581

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