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Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens

Wenya Xue Orcid Logo, Manon F. Pritchard Orcid Logo, Saira Khan Orcid Logo, Lydia Powell Orcid Logo, Joana Stokniene Orcid Logo, Jingxiang Wu Orcid Logo, Nicholas Claydon Orcid Logo, Paul Reddell Orcid Logo, David W. Thomas Orcid Logo, Katja E. Hill Orcid Logo

Journal of Oral Microbiology, Volume: 15, Issue: 1

Swansea University Author: Lydia Powell Orcid Logo

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DOI (Published version): 10.1080/20002297.2023.2241326

Abstract

Background: Peri-implantitis has become an inexorable clinical challenge in implantology. Topical immunomodulatory epoxy-tiglianes (EBCs), derived from the Queensland blushwood tree, which induce remodeling and resolve dermal infection via induction of the inflammasome and biofilm disruption, may of...

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Published in: Journal of Oral Microbiology
ISSN: 2000-2297
Published: Informa UK Limited 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64590
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Topical immunomodulatory epoxy-tiglianes (EBCs), derived from the Queensland blushwood tree, which induce remodeling and resolve dermal infection via induction of the inflammasome and biofilm disruption, may offer a novel therapeutic approach. Design: In vitro antimicrobial activity of EBC structures (EBC-46, EBC-1013 and EBC-147) against Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in minimum inhibitory concentration, growth curve and permeabilization assays were determined. Antibiofilm activity was assessed using minimum biofilm eradication concentration (MBEC) experiments. Biofilm formation and disruption assays were analyzed using confocal laser scanning microscopy, scanning electron microscopy and direct plate counting. Results: The observed antimicrobial efficacy of the tested compounds (EBC-1013 &gt; EBC-46 &gt; EBC-147) was directly related to significant membrane permeabilization and growth inhibition (p &lt; 0.05) against planktonic S. mutans and P. gingivalis. Antibiofilm activity was evident in MBEC assays, with S. mutans biofilm formation assays revealing significantly lower biomass volume and increased DEAD:LIVE cell ratio observed for EBC-1013 (p &lt; 0.05). Furthermore, biofilm disruption assays on titanium discs induced significant biofilm disruption in S. mutans and P. gingivalis (p &lt; 0.05). 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spelling v2 64590 2023-09-21 Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens 0e7e702952672bcbfdfd4974199202fb 0000-0002-8641-0160 Lydia Powell Lydia Powell true false 2023-09-21 BMS Background: Peri-implantitis has become an inexorable clinical challenge in implantology. Topical immunomodulatory epoxy-tiglianes (EBCs), derived from the Queensland blushwood tree, which induce remodeling and resolve dermal infection via induction of the inflammasome and biofilm disruption, may offer a novel therapeutic approach. Design: In vitro antimicrobial activity of EBC structures (EBC-46, EBC-1013 and EBC-147) against Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in minimum inhibitory concentration, growth curve and permeabilization assays were determined. Antibiofilm activity was assessed using minimum biofilm eradication concentration (MBEC) experiments. Biofilm formation and disruption assays were analyzed using confocal laser scanning microscopy, scanning electron microscopy and direct plate counting. Results: The observed antimicrobial efficacy of the tested compounds (EBC-1013 > EBC-46 > EBC-147) was directly related to significant membrane permeabilization and growth inhibition (p < 0.05) against planktonic S. mutans and P. gingivalis. Antibiofilm activity was evident in MBEC assays, with S. mutans biofilm formation assays revealing significantly lower biomass volume and increased DEAD:LIVE cell ratio observed for EBC-1013 (p < 0.05). Furthermore, biofilm disruption assays on titanium discs induced significant biofilm disruption in S. mutans and P. gingivalis (p < 0.05). Conclusions: EBC-1013 is a safe, semi-synthetic, compound, demonstrating clear antimicrobial biofilm disruption potential in peri-implantitis. Journal Article Journal of Oral Microbiology 15 1 Informa UK Limited 2000-2297 Dental implants, antimicrobial therapy, peri-implantitis, titanium, biofilm, epoxy-tiglianes 31 12 2023 2023-12-31 10.1080/20002297.2023.2241326 http://dx.doi.org/10.1080/20002297.2023.2241326 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2023-10-18T17:05:42.7443216 2023-09-21T15:19:18.9041158 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Wenya Xue 0000-0002-6842-1288 1 Manon F. Pritchard 0000-0002-5135-4744 2 Saira Khan 0000-0002-3284-3198 3 Lydia Powell 0000-0002-8641-0160 4 Joana Stokniene 0000-0002-8836-4209 5 Jingxiang Wu 0009-0008-8907-7368 6 Nicholas Claydon 0000-0002-4151-1515 7 Paul Reddell 0000-0002-0993-8957 8 David W. Thomas 0000-0001-7319-5820 9 Katja E. Hill 0000-0002-8590-0117 10 64590__28823__131e7e98e37f4934abd7ac183f2be9c7.pdf 64590.VOR.pdf 2023-10-18T17:04:02.2518253 Output 12669189 application/pdf Version of Record true © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0). true eng https://creativecommons.org/licenses/by/4.0/
title Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
spellingShingle Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
Lydia Powell
title_short Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
title_full Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
title_fullStr Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
title_full_unstemmed Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
title_sort Defining in vitro topical antimicrobial and antibiofilm activity of epoxy-tigliane structures against oral pathogens
author_id_str_mv 0e7e702952672bcbfdfd4974199202fb
author_id_fullname_str_mv 0e7e702952672bcbfdfd4974199202fb_***_Lydia Powell
author Lydia Powell
author2 Wenya Xue
Manon F. Pritchard
Saira Khan
Lydia Powell
Joana Stokniene
Jingxiang Wu
Nicholas Claydon
Paul Reddell
David W. Thomas
Katja E. Hill
format Journal article
container_title Journal of Oral Microbiology
container_volume 15
container_issue 1
publishDate 2023
institution Swansea University
issn 2000-2297
doi_str_mv 10.1080/20002297.2023.2241326
publisher Informa UK Limited
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://dx.doi.org/10.1080/20002297.2023.2241326
document_store_str 1
active_str 0
description Background: Peri-implantitis has become an inexorable clinical challenge in implantology. Topical immunomodulatory epoxy-tiglianes (EBCs), derived from the Queensland blushwood tree, which induce remodeling and resolve dermal infection via induction of the inflammasome and biofilm disruption, may offer a novel therapeutic approach. Design: In vitro antimicrobial activity of EBC structures (EBC-46, EBC-1013 and EBC-147) against Streptococcus mutans, Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in minimum inhibitory concentration, growth curve and permeabilization assays were determined. Antibiofilm activity was assessed using minimum biofilm eradication concentration (MBEC) experiments. Biofilm formation and disruption assays were analyzed using confocal laser scanning microscopy, scanning electron microscopy and direct plate counting. Results: The observed antimicrobial efficacy of the tested compounds (EBC-1013 > EBC-46 > EBC-147) was directly related to significant membrane permeabilization and growth inhibition (p < 0.05) against planktonic S. mutans and P. gingivalis. Antibiofilm activity was evident in MBEC assays, with S. mutans biofilm formation assays revealing significantly lower biomass volume and increased DEAD:LIVE cell ratio observed for EBC-1013 (p < 0.05). Furthermore, biofilm disruption assays on titanium discs induced significant biofilm disruption in S. mutans and P. gingivalis (p < 0.05). Conclusions: EBC-1013 is a safe, semi-synthetic, compound, demonstrating clear antimicrobial biofilm disruption potential in peri-implantitis.
published_date 2023-12-31T17:05:44Z
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score 11.013371

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