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The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field

Simone Azevedo Gomes, Aline Teixeira Carolino, Thais Berçot Pontes Teodoro, Gerson Adriano Silva Orcid Logo, Ricardo de Oliveira Barbosa Bitencourt, Carlos Peres Silva Orcid Logo, Abeer M. Alkhaibari Orcid Logo, Tariq Butt Orcid Logo, Richard Ian Samuels Orcid Logo

Journal of Fungi, Volume: 9, Issue: 7, Start page: 759

Swansea University Author: Tariq Butt Orcid Logo

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DOI (Published version): 10.3390/jof9070759

Abstract

Entomopathogenic fungi are promising as an environmentally benign alternative to chemical pesticides for mosquito control. The current study investigated the virulence of Metarhizium anisopliae blastospores against Aedes aegypti under both laboratory and field conditions. Virulence bioassays of coni...

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Published in: Journal of Fungi
ISSN: 2309-608X
Published: MDPI AG 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa64132
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Virulence bioassays of conidia and blastospores were conducted in the laboratory, while field simulation bioassays were conducted under two conditions: totally shaded (TS) or partially shaded (PS). In the first bioassay (zero h), the larvae were added to the cups shortly after the preparation of the blastospores, and in the subsequent assays, larvae were added to the cups 3, 6, 9, and 12 days later. The survival of the larvae exposed to blastospores in the laboratory was zero on day two, as was the case for the larvae exposed to conidia on the sixth day. Under TS conditions, zero survival was seen on the third day of the bioassay. Under PS conditions, low survival rates were recorded on day 7. For the persistence bioassay under PS conditions, low survival rates were also observed. Metarhizium anisopliae blastospores were more virulent to Ae. aegypti larvae than conidia in the laboratory. Blastospores remained virulent under field simulation conditions. 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spelling 2023-10-02T10:25:52.5486726 v2 64132 2023-08-24 The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field 85d1c2ddde272a1176e74978e25ebece 0000-0002-8789-9543 Tariq Butt Tariq Butt true false 2023-08-24 BGPS Entomopathogenic fungi are promising as an environmentally benign alternative to chemical pesticides for mosquito control. The current study investigated the virulence of Metarhizium anisopliae blastospores against Aedes aegypti under both laboratory and field conditions. Virulence bioassays of conidia and blastospores were conducted in the laboratory, while field simulation bioassays were conducted under two conditions: totally shaded (TS) or partially shaded (PS). In the first bioassay (zero h), the larvae were added to the cups shortly after the preparation of the blastospores, and in the subsequent assays, larvae were added to the cups 3, 6, 9, and 12 days later. The survival of the larvae exposed to blastospores in the laboratory was zero on day two, as was the case for the larvae exposed to conidia on the sixth day. Under TS conditions, zero survival was seen on the third day of the bioassay. Under PS conditions, low survival rates were recorded on day 7. For the persistence bioassay under PS conditions, low survival rates were also observed. Metarhizium anisopliae blastospores were more virulent to Ae. aegypti larvae than conidia in the laboratory. Blastospores remained virulent under field simulation conditions. However, virulence rapidly declined from the third day of field bioassays. Formulating blastospores in vegetable oil could protect these propagules when applied under adverse conditions. This is the first time that blastospores have been tested against mosquito larvae under simulated field conditions, and the current study could be the basis for the development of a new biological control agent. Journal Article Journal of Fungi 9 7 759 MDPI AG 2309-608X biological control; entomopathogenic fungi; virulence; persistence 18 7 2023 2023-07-18 10.3390/jof9070759 http://dx.doi.org/10.3390/jof9070759 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University Another institution paid the OA fee This research was funded by FAPERJ (Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro) grant numbers: E-26/201.336/2016; E-26/210.120/2022; EI-260003/000339/2023. RIS (productivity grant number: 309975/2021-2) and CPS are CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) research fellows. 2023-10-02T10:25:52.5486726 2023-08-24T15:24:16.6798454 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Simone Azevedo Gomes 1 Aline Teixeira Carolino 2 Thais Berçot Pontes Teodoro 3 Gerson Adriano Silva 0000-0002-1445-9758 4 Ricardo de Oliveira Barbosa Bitencourt 5 Carlos Peres Silva 0000-0001-5112-7050 6 Abeer M. Alkhaibari 0000-0003-4376-2438 7 Tariq Butt 0000-0002-8789-9543 8 Richard Ian Samuels 0000-0003-1814-2456 9 64132__28375__badd84b088b944209fb3938866e4fb67.pdf 64132.pdf 2023-08-24T15:26:38.7883381 Output 1043977 application/pdf Version of Record true This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license. false eng http://creativecommons.org/licenses/by/4.0/
title The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
spellingShingle The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
Tariq Butt
title_short The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
title_full The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
title_fullStr The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
title_full_unstemmed The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
title_sort The Potential of Metarhizium anisopliae Blastospores to Control Aedes aegypti Larvae in the Field
author_id_str_mv 85d1c2ddde272a1176e74978e25ebece
author_id_fullname_str_mv 85d1c2ddde272a1176e74978e25ebece_***_Tariq Butt
author Tariq Butt
author2 Simone Azevedo Gomes
Aline Teixeira Carolino
Thais Berçot Pontes Teodoro
Gerson Adriano Silva
Ricardo de Oliveira Barbosa Bitencourt
Carlos Peres Silva
Abeer M. Alkhaibari
Tariq Butt
Richard Ian Samuels
format Journal article
container_title Journal of Fungi
container_volume 9
container_issue 7
container_start_page 759
publishDate 2023
institution Swansea University
issn 2309-608X
doi_str_mv 10.3390/jof9070759
publisher MDPI AG
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url http://dx.doi.org/10.3390/jof9070759
document_store_str 1
active_str 0
description Entomopathogenic fungi are promising as an environmentally benign alternative to chemical pesticides for mosquito control. The current study investigated the virulence of Metarhizium anisopliae blastospores against Aedes aegypti under both laboratory and field conditions. Virulence bioassays of conidia and blastospores were conducted in the laboratory, while field simulation bioassays were conducted under two conditions: totally shaded (TS) or partially shaded (PS). In the first bioassay (zero h), the larvae were added to the cups shortly after the preparation of the blastospores, and in the subsequent assays, larvae were added to the cups 3, 6, 9, and 12 days later. The survival of the larvae exposed to blastospores in the laboratory was zero on day two, as was the case for the larvae exposed to conidia on the sixth day. Under TS conditions, zero survival was seen on the third day of the bioassay. Under PS conditions, low survival rates were recorded on day 7. For the persistence bioassay under PS conditions, low survival rates were also observed. Metarhizium anisopliae blastospores were more virulent to Ae. aegypti larvae than conidia in the laboratory. Blastospores remained virulent under field simulation conditions. However, virulence rapidly declined from the third day of field bioassays. Formulating blastospores in vegetable oil could protect these propagules when applied under adverse conditions. This is the first time that blastospores have been tested against mosquito larvae under simulated field conditions, and the current study could be the basis for the development of a new biological control agent.
published_date 2023-07-18T02:41:34Z
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