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Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs

Nattinee Kittiwan, Jessica K. Calland Orcid Logo, Evangelos Mourkas Orcid Logo, Matthew Hitchings Orcid Logo, Susan Murray Orcid Logo, Pakpoom Tadee Orcid Logo, Phacharaporn Tadee Orcid Logo, Kwanjit Duangsonk Orcid Logo, Guillaume Meric Orcid Logo, Samuel K. Sheppard Orcid Logo, Prapas Patchanee Orcid Logo, Ben Pascoe Orcid Logo

Microbial Genomics, Volume: 8, Issue: 11

Swansea University Authors: Matthew Hitchings Orcid Logo, Ben Pascoe Orcid Logo

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DOI (Published version): 10.1099/mgen.0.000882

Abstract

Streptococcus suis is a leading cause of bacterial meningitis in South-East Asia, with frequent zoonotic transfer to humans associated with close contact with pigs. A small number of invasive lineages are responsible for endemic infection in the swine industry, causing considerable global economic l...

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Published in: Microbial Genomics
ISSN: 2057-5858
Published: Microbiology Society 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa63744
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spelling 2023-07-14T15:31:19.6425315 v2 63744 2023-06-28 Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 4660c0eb7e6bfd796cd749ae713ea558 0000-0001-6376-5121 Ben Pascoe Ben Pascoe true false 2023-06-28 MEDS Streptococcus suis is a leading cause of bacterial meningitis in South-East Asia, with frequent zoonotic transfer to humans associated with close contact with pigs. A small number of invasive lineages are responsible for endemic infection in the swine industry, causing considerable global economic losses. A lack of surveillance and a rising trend in clinical treatment failure has raised concerns of growing antimicrobial resistance (AMR) among invasive S. suis. Gene flow between healthy and disease isolates is poorly understood and, in this study, we sample and sequence a collection of isolates predominantly from healthy pigs in Chiang Mai province, Northern Thailand. Pangenome characterization identified extensive genetic diversity and frequent AMR carriage in isolates from healthy pigs. Multiple AMR genes were identified, conferring resistance to aminoglycosides, lincosamides, tetracycline and macrolides. All isolates were non-susceptible to three or more different antimicrobial classes, and 75 % of non-serotype 2 isolates were non-susceptible to six or more classes (compared to 37.5 % of serotype 2 isolates). AMR genes were found on integrative and conjugative elements previously observed in other species, suggesting a mobile gene pool that can be accessed by invasive disease isolates. This article contains data hosted by Microreact. Journal Article Microbial Genomics 8 11 Microbiology Society 2057-5858 Antimicrobial resistance , gene pool transmission , meningitis , One Health, Streptococcus suis 3 11 2022 2022-11-03 10.1099/mgen.0.000882 http://dx.doi.org/10.1099/mgen.0.000882 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University Another institution paid the OA fee This study was supported by the: Medical Research Council (Award MR/V001213/1), Principle Award Recipient: Ben Pascoe, Medical Research Council (Award MR/T030062/1), Principle Award Recipient: Samuel K Sheppard. 2023-07-14T15:31:19.6425315 2023-06-28T15:37:56.4024312 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Nattinee Kittiwan 1 Jessica K. Calland 0000-0001-8888-0812 2 Evangelos Mourkas 0000-0002-7411-4743 3 Matthew Hitchings 0000-0002-5527-4709 4 Susan Murray 0000-0002-3310-651x 5 Pakpoom Tadee 0000-0003-3171-9678 6 Phacharaporn Tadee 0000-0002-0889-3291 7 Kwanjit Duangsonk 0000-0002-3325-1688 8 Guillaume Meric 0000-0001-6288-9958 9 Samuel K. Sheppard 0000-0001-6901-3203 10 Prapas Patchanee 0000-0001-7690-0046 11 Ben Pascoe 0000-0001-6376-5121 12 63744__28095__c9c44707e4c548849ec0ad2dcd08b734.pdf 63744.pdf 2023-07-12T14:33:08.5328250 Output 4858651 application/pdf Version of Record true This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. true eng https://creativecommons.org/licenses/by/4.0/deed.ast
title Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
spellingShingle Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
Matthew Hitchings
Ben Pascoe
title_short Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
title_full Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
title_fullStr Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
title_full_unstemmed Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
title_sort Genetic diversity and variation in antimicrobial-resistance determinants of non-serotype 2 Streptococcus suis isolates from healthy pigs
author_id_str_mv be98847c72c14a731c4a6b7bc02b3bcf
4660c0eb7e6bfd796cd749ae713ea558
author_id_fullname_str_mv be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
4660c0eb7e6bfd796cd749ae713ea558_***_Ben Pascoe
author Matthew Hitchings
Ben Pascoe
author2 Nattinee Kittiwan
Jessica K. Calland
Evangelos Mourkas
Matthew Hitchings
Susan Murray
Pakpoom Tadee
Phacharaporn Tadee
Kwanjit Duangsonk
Guillaume Meric
Samuel K. Sheppard
Prapas Patchanee
Ben Pascoe
format Journal article
container_title Microbial Genomics
container_volume 8
container_issue 11
publishDate 2022
institution Swansea University
issn 2057-5858
doi_str_mv 10.1099/mgen.0.000882
publisher Microbiology Society
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://dx.doi.org/10.1099/mgen.0.000882
document_store_str 1
active_str 0
description Streptococcus suis is a leading cause of bacterial meningitis in South-East Asia, with frequent zoonotic transfer to humans associated with close contact with pigs. A small number of invasive lineages are responsible for endemic infection in the swine industry, causing considerable global economic losses. A lack of surveillance and a rising trend in clinical treatment failure has raised concerns of growing antimicrobial resistance (AMR) among invasive S. suis. Gene flow between healthy and disease isolates is poorly understood and, in this study, we sample and sequence a collection of isolates predominantly from healthy pigs in Chiang Mai province, Northern Thailand. Pangenome characterization identified extensive genetic diversity and frequent AMR carriage in isolates from healthy pigs. Multiple AMR genes were identified, conferring resistance to aminoglycosides, lincosamides, tetracycline and macrolides. All isolates were non-susceptible to three or more different antimicrobial classes, and 75 % of non-serotype 2 isolates were non-susceptible to six or more classes (compared to 37.5 % of serotype 2 isolates). AMR genes were found on integrative and conjugative elements previously observed in other species, suggesting a mobile gene pool that can be accessed by invasive disease isolates. This article contains data hosted by Microreact.
published_date 2022-11-03T08:22:39Z
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