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Pili Formation in Filamentous Cyanobacteria / CHARLEY ROBINSON

Swansea University Author: CHARLEY ROBINSON

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Abstract

Pili are flagella-like appendages that can be found on the surface of bacteria. Type 4 pili (T4P) are a form of pili produced that aid in functions such as cell motility, which cyanobacteria rely on due to their lack of flagella. This is carried out through various Pil proteins which are responsible...

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Published: Swansea, Wales, UK 2023
Institution: Swansea University
Degree level: Master of Research
Degree name: MRes
Supervisor: Rivera, Miguel. L., Llewellyn, Carole. and Ortiz-Urquiza, Almudena.
URI: https://cronfa.swan.ac.uk/Record/cronfa63572
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first_indexed 2023-06-02T13:11:11Z
last_indexed 2023-06-02T13:11:11Z
id cronfa63572
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spelling v2 63572 2023-06-02 Pili Formation in Filamentous Cyanobacteria 50a54b639af8b595473386fb512b0c6b CHARLEY ROBINSON CHARLEY ROBINSON true false 2023-06-02 Pili are flagella-like appendages that can be found on the surface of bacteria. Type 4 pili (T4P) are a form of pili produced that aid in functions such as cell motility, which cyanobacteria rely on due to their lack of flagella. This is carried out through various Pil proteins which are responsible for mechanisms such as pilus assembly, extension/retraction of the pilus rod, along with others working as response regulators for twitching motility. To investigate this, the filamentous species Chlorogloeopsis fritschii PCC 6912 was used to observe gene expression for these Pil proteins to understand the phototactic response of pili formation under UV-B and far-red light as these wavelengths of light can be found throughout their natural environment. Under UV-B, 64.29% showed >75% increase in gene expression with 40.0% of these pil genes showing significant upregulation. Under far-red, 45.16% showed a >75% decrease with 51.6% showing significant downregulation, specifically impacting the Pil proteins responsible for the formation of the pilus rod. These results suggest a phototactic response of C.fritschii PCC6912 with regards to pili formation, however not being triggered by all wavelengths of light that could be found throughout their habitat. E-Thesis Swansea, Wales, UK Filamentous cyanobacteria, pili, UV-B, far-red, Chlorogloeopsis fritschii 11 5 2023 2023-05-11 COLLEGE NANME COLLEGE CODE Swansea University Rivera, Miguel. L., Llewellyn, Carole. and Ortiz-Urquiza, Almudena. Master of Research MRes 2023-10-27T15:58:18.5444776 2023-06-02T14:09:00.1860892 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences CHARLEY ROBINSON 1 63572__27686__50842c6c445b44539e6a6a5c0f755729.pdf 2023_Robinson_C.final.63572.pdf 2023-06-02T14:25:23.4090529 Output 1095809 application/pdf E-Thesis – open access true Copyright: The Author, Charley Robinson, 2023. true eng
title Pili Formation in Filamentous Cyanobacteria
spellingShingle Pili Formation in Filamentous Cyanobacteria
CHARLEY ROBINSON
title_short Pili Formation in Filamentous Cyanobacteria
title_full Pili Formation in Filamentous Cyanobacteria
title_fullStr Pili Formation in Filamentous Cyanobacteria
title_full_unstemmed Pili Formation in Filamentous Cyanobacteria
title_sort Pili Formation in Filamentous Cyanobacteria
author_id_str_mv 50a54b639af8b595473386fb512b0c6b
author_id_fullname_str_mv 50a54b639af8b595473386fb512b0c6b_***_CHARLEY ROBINSON
author CHARLEY ROBINSON
author2 CHARLEY ROBINSON
format E-Thesis
publishDate 2023
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Pili are flagella-like appendages that can be found on the surface of bacteria. Type 4 pili (T4P) are a form of pili produced that aid in functions such as cell motility, which cyanobacteria rely on due to their lack of flagella. This is carried out through various Pil proteins which are responsible for mechanisms such as pilus assembly, extension/retraction of the pilus rod, along with others working as response regulators for twitching motility. To investigate this, the filamentous species Chlorogloeopsis fritschii PCC 6912 was used to observe gene expression for these Pil proteins to understand the phototactic response of pili formation under UV-B and far-red light as these wavelengths of light can be found throughout their natural environment. Under UV-B, 64.29% showed >75% increase in gene expression with 40.0% of these pil genes showing significant upregulation. Under far-red, 45.16% showed a >75% decrease with 51.6% showing significant downregulation, specifically impacting the Pil proteins responsible for the formation of the pilus rod. These results suggest a phototactic response of C.fritschii PCC6912 with regards to pili formation, however not being triggered by all wavelengths of light that could be found throughout their habitat.
published_date 2023-05-11T15:58:16Z
_version_ 1780921083443019776
score 11.037056

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