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Different housing conditions for zebrafish: What are the effects?

Priscila Fernandes, Carlos Garcia De Leaniz Orcid Logo, Fulvio A.M. Freire, Vanessa A.M. Silveira, Ana C. Luchiari

Behavioural Processes, Volume: 209, Start page: 104886

Swansea University Authors: Priscila Fernandes, Carlos Garcia De Leaniz Orcid Logo

  • Accepted Manuscript under embargo until: 5th November 2024

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DOI (Published version): 10.1016/j.beproc.2023.104886

Abstract

Zebrafish is a popular experimental model in several research areas but little is known about the effects of using different strains or housing conditions. Poor control of genetic background and housing conditions could affect experimental results and data reproducibility. Here we investigated the e...

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Published in: Behavioural Processes
ISSN: 0376-6357
Published: Elsevier BV 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa63384
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spelling v2 63384 2023-05-10 Different housing conditions for zebrafish: What are the effects? 9fd85c26262b7ff8393428ed41f29cff Priscila Fernandes Priscila Fernandes true false 1c70acd0fd64edb0856b7cf34393ab02 0000-0003-1650-2729 Carlos Garcia De Leaniz Carlos Garcia De Leaniz true false 2023-05-10 FGSEN Zebrafish is a popular experimental model in several research areas but little is known about the effects of using different strains or housing conditions. Poor control of genetic background and housing conditions could affect experimental results and data reproducibility. Here we investigated the effects of two possible sources of variation on zebrafish behaviour: fish origin and environmental parameters (light intensity, water temperature and noise). Zebrafish behaviour was then examined using the ‘novel tank test’, one of the most common paradigms used to assess anxiety-like behaviours in zebrafish. Our results show that an increase in light intensity alters fish behaviour, particularly freezing duration and distance from the bottom of the tank, indicating increased anxiety. Swimming activity increased at the lowest temperature (25 °C). However, different levels of background noise did not cause any significant changes in behaviour. Differences were also found between zebrafish strains and populations: while the AB strain from laboratory 1 was minimally influenced by variation in holding conditions, the AB strain from laboratory 2 was highly affected by changes in temperature, light, and background noise. Our study shows that variation in strains and holding conditions can significantly influence the results of behavioural testing and should be carefully considered in the experimental design and properly reported to improve data interpretation and reproducibility. Journal Article Behavioural Processes 209 104886 Elsevier BV 0376-6357 1 6 2023 2023-06-01 10.1016/j.beproc.2023.104886 http://dx.doi.org/10.1016/j.beproc.2023.104886 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University this study was funded by a Royal Society Newton Mobility grant to ACL hosted by Professor Sonia Consuegra and CGL at CSAR-Swansea University (Grant reference number: NA160190) and Coordenaç ̃ao de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). ACL is supported by Conselho Nacional de Desenvolvimento Científico e Tecnol ́ogico (CNPq) 306207/2020–6 2023-07-18T17:18:26.6425583 2023-05-10T10:08:43.2039361 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Priscila Fernandes 1 Carlos Garcia De Leaniz 0000-0003-1650-2729 2 Fulvio A.M. Freire 3 Vanessa A.M. Silveira 4 Ana C. Luchiari 5 Under embargo Under embargo 2023-05-11T13:35:43.6209277 Output 208843 application/pdf Accepted Manuscript true 2024-11-05T00:00:00.0000000 Accepted manuscript distributed under CC BY-NC-ND licence. true eng
title Different housing conditions for zebrafish: What are the effects?
spellingShingle Different housing conditions for zebrafish: What are the effects?
Priscila Fernandes
Carlos Garcia De Leaniz
title_short Different housing conditions for zebrafish: What are the effects?
title_full Different housing conditions for zebrafish: What are the effects?
title_fullStr Different housing conditions for zebrafish: What are the effects?
title_full_unstemmed Different housing conditions for zebrafish: What are the effects?
title_sort Different housing conditions for zebrafish: What are the effects?
author_id_str_mv 9fd85c26262b7ff8393428ed41f29cff
1c70acd0fd64edb0856b7cf34393ab02
author_id_fullname_str_mv 9fd85c26262b7ff8393428ed41f29cff_***_Priscila Fernandes
1c70acd0fd64edb0856b7cf34393ab02_***_Carlos Garcia De Leaniz
author Priscila Fernandes
Carlos Garcia De Leaniz
author2 Priscila Fernandes
Carlos Garcia De Leaniz
Fulvio A.M. Freire
Vanessa A.M. Silveira
Ana C. Luchiari
format Journal article
container_title Behavioural Processes
container_volume 209
container_start_page 104886
publishDate 2023
institution Swansea University
issn 0376-6357
doi_str_mv 10.1016/j.beproc.2023.104886
publisher Elsevier BV
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url http://dx.doi.org/10.1016/j.beproc.2023.104886
document_store_str 0
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description Zebrafish is a popular experimental model in several research areas but little is known about the effects of using different strains or housing conditions. Poor control of genetic background and housing conditions could affect experimental results and data reproducibility. Here we investigated the effects of two possible sources of variation on zebrafish behaviour: fish origin and environmental parameters (light intensity, water temperature and noise). Zebrafish behaviour was then examined using the ‘novel tank test’, one of the most common paradigms used to assess anxiety-like behaviours in zebrafish. Our results show that an increase in light intensity alters fish behaviour, particularly freezing duration and distance from the bottom of the tank, indicating increased anxiety. Swimming activity increased at the lowest temperature (25 °C). However, different levels of background noise did not cause any significant changes in behaviour. Differences were also found between zebrafish strains and populations: while the AB strain from laboratory 1 was minimally influenced by variation in holding conditions, the AB strain from laboratory 2 was highly affected by changes in temperature, light, and background noise. Our study shows that variation in strains and holding conditions can significantly influence the results of behavioural testing and should be carefully considered in the experimental design and properly reported to improve data interpretation and reproducibility.
published_date 2023-06-01T17:18:21Z
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score 11.013731

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