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Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz

Catrin F. Williams Orcid Logo, Catherine Hather, Jainaba Sallah Conteh, Jingjing Zhang, Raluca Popa, Anthony Owen, Cara Jonas, Heungjae Choi Orcid Logo, Rhian M. Daniel, David Lloyd, Adrian Porch, Christopher George Orcid Logo

Biochemical and Biophysical Research Communications, Volume: 661, Pages: 89 - 98

Swansea University Authors: Raluca Popa, Anthony Owen, Cara Jonas, Christopher George Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2023.04.038

Abstract

The ubiquity of wireless electronic-device connectivity has seen microwaves emerge as one of the fastest growing forms of electromagnetic exposure. A growing evidence-base refutes the claim that wireless technologies pose no risk to human health at current safety levels designed to limit thermal (he...

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Published in: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
Published: Elsevier BV 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa63176
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spelling 2024-03-05T09:20:18.6356322 v2 63176 2023-04-17 Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz 4613db9ab6ebd3ba61869fa68c1bfd27 Raluca Popa Raluca Popa true false 4c219192fa339e8b5770117559a9d173 Anthony Owen Anthony Owen true false f6ddf74e66cbd3fb9869a4808365b9da Cara Jonas Cara Jonas true false a2e211f7bd379c81e9c393637803a0a0 0000-0001-9852-1135 Christopher George Christopher George true false 2023-04-17 MEDS The ubiquity of wireless electronic-device connectivity has seen microwaves emerge as one of the fastest growing forms of electromagnetic exposure. A growing evidence-base refutes the claim that wireless technologies pose no risk to human health at current safety levels designed to limit thermal (heating) effects. The potential impact of non-thermal effects of microwave exposure, especially in electrically-excitable tissues (e.g., heart), remains controversial. We exposed human embryonic stem-cell derived cardiomyocytes (CM), under baseline and beta-adrenergic receptor (β-AR)-stimulated conditions, to microwaves at 2.4 GHz, a frequency used extensively in wireless communication (e.g., 4G, Bluetooth™ and WiFi). To control for any effect of sample heating, experiments were done in CM subjected to matched rates of direct heating or CM maintained at 37 °C throughout experiments. Detailed profiling of the temporal and amplitude features of Ca2+ signalling in CM under these experimental conditions was reconciled with the extent and spatial clustering of apoptosis. The data show that exposure of CM to 2.4 GHz EMF eliminated the normal Ca2+ signalling response to β-AR stimulation and provoked spatially-clustered apoptosis. This is first evidence that non-thermal effects of 2.4 GHz microwaves might have profound effects on human CM function, responsiveness to activation, and survival. Journal Article Biochemical and Biophysical Research Communications 661 89 98 Elsevier BV 0006-291X Microwaves, Electric field, Non-thermal, Cardiomyocytes, Calcium, Apoptosis. 1 6 2023 2023-06-01 10.1016/j.bbrc.2023.04.038 http://dx.doi.org/10.1016/j.bbrc.2023.04.038 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University SU Library paid the OA fee (TA Institutional Deal) Welsh Government, British Heart Foundation, Wellcome Trust, European Union 2024-03-05T09:20:18.6356322 2023-04-17T14:43:58.3364652 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Catrin F. Williams 0000-0001-8619-2581 1 Catherine Hather 2 Jainaba Sallah Conteh 3 Jingjing Zhang 4 Raluca Popa 5 Anthony Owen 6 Cara Jonas 7 Heungjae Choi 0000-0003-1108-293x 8 Rhian M. Daniel 9 David Lloyd 10 Adrian Porch 11 Christopher George 0000-0001-9852-1135 12 63176__27436__ccbd7443918e4ea5b55d1309e39467cf.pdf 63176.pdf 2023-05-11T13:19:25.7211962 Output 2595451 application/pdf Version of Record true This is an open access article under the CC BY license http://creativecommons.org/licenses/by/4.0/). true eng http://creativecommons.org/licenses/by/4.0/ 183 true X false
title Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
spellingShingle Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
Raluca Popa
Anthony Owen
Cara Jonas
Christopher George
title_short Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
title_full Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
title_fullStr Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
title_full_unstemmed Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
title_sort Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz
author_id_str_mv 4613db9ab6ebd3ba61869fa68c1bfd27
4c219192fa339e8b5770117559a9d173
f6ddf74e66cbd3fb9869a4808365b9da
a2e211f7bd379c81e9c393637803a0a0
author_id_fullname_str_mv 4613db9ab6ebd3ba61869fa68c1bfd27_***_Raluca Popa
4c219192fa339e8b5770117559a9d173_***_Anthony Owen
f6ddf74e66cbd3fb9869a4808365b9da_***_Cara Jonas
a2e211f7bd379c81e9c393637803a0a0_***_Christopher George
author Raluca Popa
Anthony Owen
Cara Jonas
Christopher George
author2 Catrin F. Williams
Catherine Hather
Jainaba Sallah Conteh
Jingjing Zhang
Raluca Popa
Anthony Owen
Cara Jonas
Heungjae Choi
Rhian M. Daniel
David Lloyd
Adrian Porch
Christopher George
format Journal article
container_title Biochemical and Biophysical Research Communications
container_volume 661
container_start_page 89
publishDate 2023
institution Swansea University
issn 0006-291X
doi_str_mv 10.1016/j.bbrc.2023.04.038
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.1016/j.bbrc.2023.04.038
document_store_str 1
active_str 0
description The ubiquity of wireless electronic-device connectivity has seen microwaves emerge as one of the fastest growing forms of electromagnetic exposure. A growing evidence-base refutes the claim that wireless technologies pose no risk to human health at current safety levels designed to limit thermal (heating) effects. The potential impact of non-thermal effects of microwave exposure, especially in electrically-excitable tissues (e.g., heart), remains controversial. We exposed human embryonic stem-cell derived cardiomyocytes (CM), under baseline and beta-adrenergic receptor (β-AR)-stimulated conditions, to microwaves at 2.4 GHz, a frequency used extensively in wireless communication (e.g., 4G, Bluetooth™ and WiFi). To control for any effect of sample heating, experiments were done in CM subjected to matched rates of direct heating or CM maintained at 37 °C throughout experiments. Detailed profiling of the temporal and amplitude features of Ca2+ signalling in CM under these experimental conditions was reconciled with the extent and spatial clustering of apoptosis. The data show that exposure of CM to 2.4 GHz EMF eliminated the normal Ca2+ signalling response to β-AR stimulation and provoked spatially-clustered apoptosis. This is first evidence that non-thermal effects of 2.4 GHz microwaves might have profound effects on human CM function, responsiveness to activation, and survival.
published_date 2023-06-01T09:25:33Z
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