Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Fernandes, Fleuriane; Silkina, Alla; PELAEZ, JOSE; Kapoore, Rahul; la, Denis de; Llewellyn, Carole

doi:10.3390/app12115429

Journal article 705 views 92 downloads

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Fleuriane Fernandes, Alla Silkina

, JOSE PELAEZ, Rahul Kapoore

, Denis de la Broise, Carole Llewellyn

Applied Sciences, Volume: 12, Issue: 11, Start page: 5429

Swansea University Authors: Fleuriane Fernandes, Alla Silkina , JOSE PELAEZ, Rahul Kapoore , Carole Llewellyn

PDF | Version of Record

© 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
Download (2.11MB)

Check full text

DOI (Published version): 10.3390/app12115429

Abstract

The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cult...

Full description

Published in:	Applied Sciences
ISSN:	2076-3417
Published:	MDPI AG 2022
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa62685

first_indexed	2023-03-08T18:45:05Z
last_indexed	2023-03-09T04:17:16Z
id	cronfa62685
recordtype	SURis
fullrecord	<?xml version="1.0"?><rfc1807><datestamp>2023-03-08T18:47:04.5269511</datestamp><bib-version>v2</bib-version><id>62685</id><entry>2023-02-18</entry><title>Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control</title><swanseaauthors><author><sid>2d794f8693a3aa36c5f333897f12a82c</sid><ORCID/><firstname>Fleuriane</firstname><surname>Fernandes</surname><name>Fleuriane Fernandes</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>216d36449db09ed98c6971a2254a2457</sid><ORCID>0000-0002-1804-8083</ORCID><firstname>Alla</firstname><surname>Silkina</surname><name>Alla Silkina</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>94eeb8ef5276b1199289e19933f390fe</sid><firstname>JOSE</firstname><surname>PELAEZ</surname><name>JOSE PELAEZ</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>5583be4600daecd670edac16f6e77e88</sid><ORCID>0000-0002-2287-0619</ORCID><firstname>Rahul</firstname><surname>Kapoore</surname><name>Rahul Kapoore</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>bcd94bda79ebf4c2c82d82dfb027a140</sid><firstname>Carole</firstname><surname>Llewellyn</surname><name>Carole Llewellyn</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2023-02-18</date><deptcode>BGPS</deptcode><abstract>The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time.</abstract><type>Journal Article</type><journal>Applied Sciences</journal><volume>12</volume><journalNumber>11</journalNumber><paginationStart>5429</paginationStart><paginationEnd/><publisher>MDPI AG</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2076-3417</issnElectronic><keywords>digestate; microalgae; pH control; ammonium; bioremediation</keywords><publishedDay>27</publishedDay><publishedMonth>5</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-05-27</publishedDate><doi>10.3390/app12115429</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences Geography and Physics School</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BGPS</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>This research was funded by the ALG-AD project funded under the INTERREG North-West Europe program (project number: NWE 520).</funders><projectreference/><lastEdited>2023-03-08T18:47:04.5269511</lastEdited><Created>2023-02-18T13:12:17.3002846</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Fleuriane</firstname><surname>Fernandes</surname><orcid/><order>1</order></author><author><firstname>Alla</firstname><surname>Silkina</surname><orcid>0000-0002-1804-8083</orcid><order>2</order></author><author><firstname>JOSE</firstname><surname>PELAEZ</surname><order>3</order></author><author><firstname>Rahul</firstname><surname>Kapoore</surname><orcid>0000-0002-2287-0619</orcid><order>4</order></author><author><firstname>Denis de la</firstname><surname>Broise</surname><order>5</order></author><author><firstname>Carole</firstname><surname>Llewellyn</surname><order>6</order></author></authors><documents><document><filename>62685__26783__cc1dba9f31e34acca9887279171979f9.pdf</filename><originalFilename>62685_VoR.pdf</originalFilename><uploaded>2023-03-08T18:45:37.5469127</uploaded><type>Output</type><contentLength>2216680</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling	2023-03-08T18:47:04.5269511 v2 62685 2023-02-18 Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control 2d794f8693a3aa36c5f333897f12a82c Fleuriane Fernandes Fleuriane Fernandes true false 216d36449db09ed98c6971a2254a2457 0000-0002-1804-8083 Alla Silkina Alla Silkina true false 94eeb8ef5276b1199289e19933f390fe JOSE PELAEZ JOSE PELAEZ true false 5583be4600daecd670edac16f6e77e88 0000-0002-2287-0619 Rahul Kapoore Rahul Kapoore true false bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 2023-02-18 BGPS The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time. Journal Article Applied Sciences 12 11 5429 MDPI AG 2076-3417 digestate; microalgae; pH control; ammonium; bioremediation 27 5 2022 2022-05-27 10.3390/app12115429 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University This research was funded by the ALG-AD project funded under the INTERREG North-West Europe program (project number: NWE 520). 2023-03-08T18:47:04.5269511 2023-02-18T13:12:17.3002846 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Fleuriane Fernandes 1 Alla Silkina 0000-0002-1804-8083 2 JOSE PELAEZ 3 Rahul Kapoore 0000-0002-2287-0619 4 Denis de la Broise 5 Carole Llewellyn 6 62685__26783__cc1dba9f31e34acca9887279171979f9.pdf 62685_VoR.pdf 2023-03-08T18:45:37.5469127 Output 2216680 application/pdf Version of Record true © 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
spellingShingle	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control Fleuriane Fernandes Alla Silkina JOSE PELAEZ Rahul Kapoore Carole Llewellyn
title_short	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_full	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_fullStr	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_full_unstemmed	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_sort	Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
author_id_str_mv	2d794f8693a3aa36c5f333897f12a82c 216d36449db09ed98c6971a2254a2457 94eeb8ef5276b1199289e19933f390fe 5583be4600daecd670edac16f6e77e88 bcd94bda79ebf4c2c82d82dfb027a140
author_id_fullname_str_mv	2d794f8693a3aa36c5f333897f12a82c_*_Fleuriane Fernandes 216d36449db09ed98c6971a2254a2457__Alla Silkina 94eeb8ef5276b1199289e19933f390fe__JOSE PELAEZ 5583be4600daecd670edac16f6e77e88__Rahul Kapoore bcd94bda79ebf4c2c82d82dfb027a140_**_Carole Llewellyn
author	Fleuriane Fernandes Alla Silkina JOSE PELAEZ Rahul Kapoore Carole Llewellyn
author2	Fleuriane Fernandes Alla Silkina JOSE PELAEZ Rahul Kapoore Denis de la Broise Carole Llewellyn
format	Journal article
container_title	Applied Sciences
container_volume	12
container_issue	11
container_start_page	5429
publishDate	2022
institution	Swansea University
issn	2076-3417
doi_str_mv	10.3390/app12115429
publisher	MDPI AG
college_str	Faculty of Science and Engineering
hierarchytype
hierarchy_top_id	facultyofscienceandengineering
hierarchy_top_title	Faculty of Science and Engineering
hierarchy_parent_id	facultyofscienceandengineering
hierarchy_parent_title	Faculty of Science and Engineering
department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str	1
active_str	0
description	The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time.
published_date	2022-05-27T20:19:55Z
_version_	1821347566195834880
score	11.04748

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Similar Items