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Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Fleuriane Fernandes, Alla Silkina Orcid Logo, JOSE PELAEZ, Rahul Kapoore Orcid Logo, Denis de la Broise, Carole Llewellyn

Applied Sciences, Volume: 12, Issue: 11, Start page: 5429

Swansea University Authors: Fleuriane Fernandes, Alla Silkina Orcid Logo, JOSE PELAEZ, Rahul Kapoore Orcid Logo, Carole Llewellyn

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DOI (Published version): 10.3390/app12115429

Abstract

The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cult...

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Published in: Applied Sciences
ISSN: 2076-3417
Published: MDPI AG 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa62685
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Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6&#x2013;6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 &#xB1; 0.001 day&#x2212;1; DW: 0.23 &#xB1; 0.02 g L&#x2212;1) and C. vulgaris (GR: 0.09 &#xB1; 0.001 day&#x2212;1; DW: 0.49 &#xB1; 0.012 g L&#x2212;1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 &#xB1; 0.033% DW and 4.91 &#xB1; 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time.</abstract><type>Journal Article</type><journal>Applied Sciences</journal><volume>12</volume><journalNumber>11</journalNumber><paginationStart>5429</paginationStart><paginationEnd/><publisher>MDPI AG</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2076-3417</issnElectronic><keywords>digestate; microalgae; pH control; ammonium; bioremediation</keywords><publishedDay>27</publishedDay><publishedMonth>5</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-05-27</publishedDate><doi>10.3390/app12115429</doi><url/><notes/><college>COLLEGE NANME</college><department>Biosciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>SBI</DepartmentCode><institution>Swansea University</institution><apcterm/><funders>This research was funded by the ALG-AD project funded under the INTERREG North-West Europe program (project number: NWE 520).</funders><projectreference/><lastEdited>2023-03-08T18:47:04.5269511</lastEdited><Created>2023-02-18T13:12:17.3002846</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Biosciences, Geography and Physics - Biosciences</level></path><authors><author><firstname>Fleuriane</firstname><surname>Fernandes</surname><orcid/><order>1</order></author><author><firstname>Alla</firstname><surname>Silkina</surname><orcid>0000-0002-1804-8083</orcid><order>2</order></author><author><firstname>JOSE</firstname><surname>PELAEZ</surname><order>3</order></author><author><firstname>Rahul</firstname><surname>Kapoore</surname><orcid>0000-0002-2287-0619</orcid><order>4</order></author><author><firstname>Denis de la</firstname><surname>Broise</surname><order>5</order></author><author><firstname>Carole</firstname><surname>Llewellyn</surname><order>6</order></author></authors><documents><document><filename>62685__26783__cc1dba9f31e34acca9887279171979f9.pdf</filename><originalFilename>62685_VoR.pdf</originalFilename><uploaded>2023-03-08T18:45:37.5469127</uploaded><type>Output</type><contentLength>2216680</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; 2022 by the authors. 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spelling 2023-03-08T18:47:04.5269511 v2 62685 2023-02-18 Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control 2d794f8693a3aa36c5f333897f12a82c Fleuriane Fernandes Fleuriane Fernandes true false 216d36449db09ed98c6971a2254a2457 0000-0002-1804-8083 Alla Silkina Alla Silkina true false 94eeb8ef5276b1199289e19933f390fe JOSE PELAEZ JOSE PELAEZ true false 5583be4600daecd670edac16f6e77e88 0000-0002-2287-0619 Rahul Kapoore Rahul Kapoore true false bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 2023-02-18 SBI The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time. Journal Article Applied Sciences 12 11 5429 MDPI AG 2076-3417 digestate; microalgae; pH control; ammonium; bioremediation 27 5 2022 2022-05-27 10.3390/app12115429 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University This research was funded by the ALG-AD project funded under the INTERREG North-West Europe program (project number: NWE 520). 2023-03-08T18:47:04.5269511 2023-02-18T13:12:17.3002846 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Fleuriane Fernandes 1 Alla Silkina 0000-0002-1804-8083 2 JOSE PELAEZ 3 Rahul Kapoore 0000-0002-2287-0619 4 Denis de la Broise 5 Carole Llewellyn 6 62685__26783__cc1dba9f31e34acca9887279171979f9.pdf 62685_VoR.pdf 2023-03-08T18:45:37.5469127 Output 2216680 application/pdf Version of Record true © 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
spellingShingle Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
Fleuriane Fernandes
Alla Silkina
JOSE PELAEZ
Rahul Kapoore
Carole Llewellyn
title_short Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_full Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_fullStr Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_full_unstemmed Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
title_sort Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control
author_id_str_mv 2d794f8693a3aa36c5f333897f12a82c
216d36449db09ed98c6971a2254a2457
94eeb8ef5276b1199289e19933f390fe
5583be4600daecd670edac16f6e77e88
bcd94bda79ebf4c2c82d82dfb027a140
author_id_fullname_str_mv 2d794f8693a3aa36c5f333897f12a82c_***_Fleuriane Fernandes
216d36449db09ed98c6971a2254a2457_***_Alla Silkina
94eeb8ef5276b1199289e19933f390fe_***_JOSE PELAEZ
5583be4600daecd670edac16f6e77e88_***_Rahul Kapoore
bcd94bda79ebf4c2c82d82dfb027a140_***_Carole Llewellyn
author Fleuriane Fernandes
Alla Silkina
JOSE PELAEZ
Rahul Kapoore
Carole Llewellyn
author2 Fleuriane Fernandes
Alla Silkina
JOSE PELAEZ
Rahul Kapoore
Denis de la Broise
Carole Llewellyn
format Journal article
container_title Applied Sciences
container_volume 12
container_issue 11
container_start_page 5429
publishDate 2022
institution Swansea University
issn 2076-3417
doi_str_mv 10.3390/app12115429
publisher MDPI AG
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time.
published_date 2022-05-27T04:22:30Z
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score 11.013148

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