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Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn / Pierre Bourdon

Swansea University Author: Pierre Bourdon

  • E-Thesis – open access under embargo until: 20th January 2028

DOI (Published version): 10.23889/SUthesis.62670

Abstract

Wireworms, the larvae of the click beetle are a major polyphagous soil pest, damaging crops such as corn, wheat, or potatoes. The withdrawal of registration of many chemical insecticides left farmers with a limited number of solutions against wireworms and soil pests. Entomopathogenic fungi (EPF) ca...

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Published: Swansea 2023
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: Butt, Tariq ; Wilson, Rory ; Baxter, Ian ; Myrta, Arben
URI: https://cronfa.swan.ac.uk/Record/cronfa62670
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To override these limitations, stress and kill strategies were tested, combining spinosad and different Metarhizium strain against three different wireworm species from the genera Agriotes. Combining spinosad and M. brunneum could increase the mortality of the EPF strains; yet, the susceptibility of the Agriotes species differs for each combination (Chapter I). The aim of the PhD was to develop new integrated pest management strategies against wireworms to avoid important corn yield losses. Developing pest management strategies using EPF seemed unreliable; thus, fungal volatile organic compounds (VOCs) were tested for their fumigant and attractant/ repellent activity (Chapter II and III). In Chapter II, fumigation using 1-octen-3-ol and 3-octanone was tested against wireworm (Agriotes lineatus), corn rootworm (Diabrotica virgifera virgifera) and garden chafer (Phyllopertha horticola). The VOCs were highly efficacious in a closed environment (Chapter II); yet, in open containers, no mortality was observed (Chapter III). In this experiment, the VOCs had attractant/ repellent properties depending on the species. In silico study, confirmed that corn rootworm could detect and recognize the VOCs with their odorant binding proteins. As odorant binding proteins are well conserved between species, it is expected that wireworm and garden chafer can also detect and recognize the VOCs. In chapter IV, the VOCs were formulated as slow-release granules. These granules facilitate application of the VOCs in the field and increase the remanence of the VOCs in the soil. In fact, to avoid economic damage, wireworms need to be repelled from maize seedlings during the three first weeks of growth. Four doses of 1-octen-3-ol cyclodextrin granules were tested to reduce wireworm damage on maize. The lowest dose (2.26kg/ha) was attractive and increased damage; but doses of 4.52kg/ha or higher, significantly reduced damage for 18 days. 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spelling 2023-02-16T16:23:44.8382180 v2 62670 2023-02-15 Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn ff34dba02fbc659ca5cd2200f815ca38 Pierre Bourdon Pierre Bourdon true false 2023-02-15 SBI Wireworms, the larvae of the click beetle are a major polyphagous soil pest, damaging crops such as corn, wheat, or potatoes. The withdrawal of registration of many chemical insecticides left farmers with a limited number of solutions against wireworms and soil pests. Entomopathogenic fungi (EPF) can be useful against wireworm; yet they are slow acting and species dependent. To override these limitations, stress and kill strategies were tested, combining spinosad and different Metarhizium strain against three different wireworm species from the genera Agriotes. Combining spinosad and M. brunneum could increase the mortality of the EPF strains; yet, the susceptibility of the Agriotes species differs for each combination (Chapter I). The aim of the PhD was to develop new integrated pest management strategies against wireworms to avoid important corn yield losses. Developing pest management strategies using EPF seemed unreliable; thus, fungal volatile organic compounds (VOCs) were tested for their fumigant and attractant/ repellent activity (Chapter II and III). In Chapter II, fumigation using 1-octen-3-ol and 3-octanone was tested against wireworm (Agriotes lineatus), corn rootworm (Diabrotica virgifera virgifera) and garden chafer (Phyllopertha horticola). The VOCs were highly efficacious in a closed environment (Chapter II); yet, in open containers, no mortality was observed (Chapter III). In this experiment, the VOCs had attractant/ repellent properties depending on the species. In silico study, confirmed that corn rootworm could detect and recognize the VOCs with their odorant binding proteins. As odorant binding proteins are well conserved between species, it is expected that wireworm and garden chafer can also detect and recognize the VOCs. In chapter IV, the VOCs were formulated as slow-release granules. These granules facilitate application of the VOCs in the field and increase the remanence of the VOCs in the soil. In fact, to avoid economic damage, wireworms need to be repelled from maize seedlings during the three first weeks of growth. Four doses of 1-octen-3-ol cyclodextrin granules were tested to reduce wireworm damage on maize. The lowest dose (2.26kg/ha) was attractive and increased damage; but doses of 4.52kg/ha or higher, significantly reduced damage for 18 days. The granule formulation needs to be optimized to increase the duration of the protection, but early indications suggest that VOCs granules appear to be a possible candidate to achieve wireworm control. E-Thesis Swansea Wireworm, pest management, microbials, volatiles organic compounds, entomopathogenic fungi, soil pest 20 1 2023 2023-01-20 10.23889/SUthesis.62670 ORCiD identifier: https://orcid.org/0000-0003-1884-137X COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University Butt, Tariq ; Wilson, Rory ; Baxter, Ian ; Myrta, Arben Doctoral Ph.D Certis-Belchim 2023-02-16T16:23:44.8382180 2023-02-15T16:29:35.6956778 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Pierre Bourdon 1 Under embargo Under embargo 2023-02-16T16:18:37.2521130 Output 3227893 application/pdf E-Thesis – open access true 2028-01-20T00:00:00.0000000 Copyright: The author, Pierre-Antoine Bourdon, 2023. true eng
title Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
spellingShingle Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
Pierre Bourdon
title_short Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
title_full Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
title_fullStr Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
title_full_unstemmed Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
title_sort Development of novel integrated pest management strategies against wireworms (Agriotes spp.) in French corn
author_id_str_mv ff34dba02fbc659ca5cd2200f815ca38
author_id_fullname_str_mv ff34dba02fbc659ca5cd2200f815ca38_***_Pierre Bourdon
author Pierre Bourdon
author2 Pierre Bourdon
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publishDate 2023
institution Swansea University
doi_str_mv 10.23889/SUthesis.62670
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description Wireworms, the larvae of the click beetle are a major polyphagous soil pest, damaging crops such as corn, wheat, or potatoes. The withdrawal of registration of many chemical insecticides left farmers with a limited number of solutions against wireworms and soil pests. Entomopathogenic fungi (EPF) can be useful against wireworm; yet they are slow acting and species dependent. To override these limitations, stress and kill strategies were tested, combining spinosad and different Metarhizium strain against three different wireworm species from the genera Agriotes. Combining spinosad and M. brunneum could increase the mortality of the EPF strains; yet, the susceptibility of the Agriotes species differs for each combination (Chapter I). The aim of the PhD was to develop new integrated pest management strategies against wireworms to avoid important corn yield losses. Developing pest management strategies using EPF seemed unreliable; thus, fungal volatile organic compounds (VOCs) were tested for their fumigant and attractant/ repellent activity (Chapter II and III). In Chapter II, fumigation using 1-octen-3-ol and 3-octanone was tested against wireworm (Agriotes lineatus), corn rootworm (Diabrotica virgifera virgifera) and garden chafer (Phyllopertha horticola). The VOCs were highly efficacious in a closed environment (Chapter II); yet, in open containers, no mortality was observed (Chapter III). In this experiment, the VOCs had attractant/ repellent properties depending on the species. In silico study, confirmed that corn rootworm could detect and recognize the VOCs with their odorant binding proteins. As odorant binding proteins are well conserved between species, it is expected that wireworm and garden chafer can also detect and recognize the VOCs. In chapter IV, the VOCs were formulated as slow-release granules. These granules facilitate application of the VOCs in the field and increase the remanence of the VOCs in the soil. In fact, to avoid economic damage, wireworms need to be repelled from maize seedlings during the three first weeks of growth. Four doses of 1-octen-3-ol cyclodextrin granules were tested to reduce wireworm damage on maize. The lowest dose (2.26kg/ha) was attractive and increased damage; but doses of 4.52kg/ha or higher, significantly reduced damage for 18 days. The granule formulation needs to be optimized to increase the duration of the protection, but early indications suggest that VOCs granules appear to be a possible candidate to achieve wireworm control.
published_date 2023-01-20T04:22:28Z
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score 11.013371

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