No Cover Image

Journal article 530 views 48 downloads

A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips

Miranda Walker, Qi Xue, Clauvis Nji Tizi Taning, Reuben James, Guy Smagghe, Ricardo del Sol, Matthew Hitchings Orcid Logo, Paul Dyson Orcid Logo

Frontiers in Insect Science, Volume: 3

Swansea University Authors: Miranda Walker, Reuben James, Matthew Hitchings Orcid Logo, Paul Dyson Orcid Logo

  • 62479.VOR.pdf

    PDF | Version of Record

    Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0).

    Download (1.94MB)

Check full text

DOI (Published version): 10.3389/finsc.2023.1093970

Abstract

Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrat...

Full description

Published in: Frontiers in Insect Science
ISSN: 2673-8600
Published: Frontiers Media SA 2023
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa62479
Tags: Add Tag
No Tags, Be the first to tag this record!
first_indexed 2023-02-01T15:03:42Z
last_indexed 2023-03-03T04:19:42Z
id cronfa62479
recordtype SURis
fullrecord <?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>62479</id><entry>2023-02-01</entry><title>A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips</title><swanseaauthors><author><sid>83389c57d000a704fae36fda87d7ca76</sid><firstname>Miranda</firstname><surname>Walker</surname><name>Miranda Walker</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>5a0edd73a6907dc33c016e97d6e784b1</sid><firstname>Reuben</firstname><surname>James</surname><name>Reuben James</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>be98847c72c14a731c4a6b7bc02b3bcf</sid><ORCID>0000-0002-5527-4709</ORCID><firstname>Matthew</firstname><surname>Hitchings</surname><name>Matthew Hitchings</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>300e3f46b70ae83f563b24f41d00cd17</sid><ORCID>0000-0002-0558-2666</ORCID><firstname>Paul</firstname><surname>Dyson</surname><name>Paul Dyson</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2023-02-01</date><deptcode>FGMHL</deptcode><abstract>Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrated in the globally-invasive pest species Western Flower Thrips (WFT). Here we determine a very limited distribution of the two predominant bacterial symbionts of WFT, BFo1 and BFo2, among genome-sequenced insects. Moreover, we have challenged two non-target insect species with both bacterial species, namely the pollinating European bumblebee, Bombus terrestris, and an insect predator of WFT, the pirate bug Orius laevigatus. Our data indicate that whereas BFo1 could establish itself in both these non-natural hosts, albeit with no significant effects on insect fitness, BFo2 was unable to persist in either species. In terms of biosafety, these data, together with its more specific growth requirements, vindicate the choice of BFo2 for delivery of RNAi and precision pest management of WFT.</abstract><type>Journal Article</type><journal>Frontiers in Insect Science</journal><volume>3</volume><journalNumber/><paginationStart/><paginationEnd/><publisher>Frontiers Media SA</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2673-8600</issnElectronic><keywords>symbiotic bacteria, Host-range, Western flower thrips, European bumblebee, Pirate bug</keywords><publishedDay>8</publishedDay><publishedMonth>3</publishedMonth><publishedYear>2023</publishedYear><publishedDate>2023-03-08</publishedDate><doi>10.3389/finsc.2023.1093970</doi><url>http://dx.doi.org/10.3389/finsc.2023.1093970</url><notes/><college>COLLEGE NANME</college><department>Medicine, Health and Life Science - Faculty</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>FGMHL</DepartmentCode><institution>Swansea University</institution><apcterm>External research funder(s) paid the OA fee (includes OA grants disbursed by the Library)</apcterm><funders>BB/R006148/1</funders><projectreference/><lastEdited>2024-01-08T10:33:57.6500298</lastEdited><Created>2023-02-01T15:02:21.0096065</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Biomedical Science</level></path><authors><author><firstname>Miranda</firstname><surname>Walker</surname><order>1</order></author><author><firstname>Qi</firstname><surname>Xue</surname><order>2</order></author><author><firstname>Clauvis Nji Tizi</firstname><surname>Taning</surname><order>3</order></author><author><firstname>Reuben</firstname><surname>James</surname><order>4</order></author><author><firstname>Guy</firstname><surname>Smagghe</surname><order>5</order></author><author><firstname>Ricardo del</firstname><surname>Sol</surname><order>6</order></author><author><firstname>Matthew</firstname><surname>Hitchings</surname><orcid>0000-0002-5527-4709</orcid><order>7</order></author><author><firstname>Paul</firstname><surname>Dyson</surname><orcid>0000-0002-0558-2666</orcid><order>8</order></author></authors><documents><document><filename>62479__27315__7eb81e829f554690976432e43c53b98f.pdf</filename><originalFilename>62479.VOR.pdf</originalFilename><uploaded>2023-05-03T12:11:40.0954020</uploaded><type>Output</type><contentLength>2031394</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>https://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling v2 62479 2023-02-01 A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips 83389c57d000a704fae36fda87d7ca76 Miranda Walker Miranda Walker true false 5a0edd73a6907dc33c016e97d6e784b1 Reuben James Reuben James true false be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 300e3f46b70ae83f563b24f41d00cd17 0000-0002-0558-2666 Paul Dyson Paul Dyson true false 2023-02-01 FGMHL Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrated in the globally-invasive pest species Western Flower Thrips (WFT). Here we determine a very limited distribution of the two predominant bacterial symbionts of WFT, BFo1 and BFo2, among genome-sequenced insects. Moreover, we have challenged two non-target insect species with both bacterial species, namely the pollinating European bumblebee, Bombus terrestris, and an insect predator of WFT, the pirate bug Orius laevigatus. Our data indicate that whereas BFo1 could establish itself in both these non-natural hosts, albeit with no significant effects on insect fitness, BFo2 was unable to persist in either species. In terms of biosafety, these data, together with its more specific growth requirements, vindicate the choice of BFo2 for delivery of RNAi and precision pest management of WFT. Journal Article Frontiers in Insect Science 3 Frontiers Media SA 2673-8600 symbiotic bacteria, Host-range, Western flower thrips, European bumblebee, Pirate bug 8 3 2023 2023-03-08 10.3389/finsc.2023.1093970 http://dx.doi.org/10.3389/finsc.2023.1093970 COLLEGE NANME Medicine, Health and Life Science - Faculty COLLEGE CODE FGMHL Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) BB/R006148/1 2024-01-08T10:33:57.6500298 2023-02-01T15:02:21.0096065 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Miranda Walker 1 Qi Xue 2 Clauvis Nji Tizi Taning 3 Reuben James 4 Guy Smagghe 5 Ricardo del Sol 6 Matthew Hitchings 0000-0002-5527-4709 7 Paul Dyson 0000-0002-0558-2666 8 62479__27315__7eb81e829f554690976432e43c53b98f.pdf 62479.VOR.pdf 2023-05-03T12:11:40.0954020 Output 2031394 application/pdf Version of Record true Distributed under the terms of a Creative Commons Attribution 4.0 License (CC BY 4.0). true eng https://creativecommons.org/licenses/by/4.0/
title A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
spellingShingle A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
Miranda Walker
Reuben James
Matthew Hitchings
Paul Dyson
title_short A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
title_full A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
title_fullStr A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
title_full_unstemmed A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
title_sort A narrow host-range and lack of persistence in two non-target insect species of a bacterial symbiont exploited to deliver insecticidal RNAi in Western Flower Thrips
author_id_str_mv 83389c57d000a704fae36fda87d7ca76
5a0edd73a6907dc33c016e97d6e784b1
be98847c72c14a731c4a6b7bc02b3bcf
300e3f46b70ae83f563b24f41d00cd17
author_id_fullname_str_mv 83389c57d000a704fae36fda87d7ca76_***_Miranda Walker
5a0edd73a6907dc33c016e97d6e784b1_***_Reuben James
be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
300e3f46b70ae83f563b24f41d00cd17_***_Paul Dyson
author Miranda Walker
Reuben James
Matthew Hitchings
Paul Dyson
author2 Miranda Walker
Qi Xue
Clauvis Nji Tizi Taning
Reuben James
Guy Smagghe
Ricardo del Sol
Matthew Hitchings
Paul Dyson
format Journal article
container_title Frontiers in Insect Science
container_volume 3
publishDate 2023
institution Swansea University
issn 2673-8600
doi_str_mv 10.3389/finsc.2023.1093970
publisher Frontiers Media SA
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
url http://dx.doi.org/10.3389/finsc.2023.1093970
document_store_str 1
active_str 0
description Insecticidal RNAi is a targeted pest insect population control measure. The specificity of insecticidal RNAi can theoretically be enhanced by using symbiotic bacteria with a narrow host range to deliver RNAi, an approach termed symbiont-mediated RNAi (SMR), a technology we have previously demonstrated in the globally-invasive pest species Western Flower Thrips (WFT). Here we determine a very limited distribution of the two predominant bacterial symbionts of WFT, BFo1 and BFo2, among genome-sequenced insects. Moreover, we have challenged two non-target insect species with both bacterial species, namely the pollinating European bumblebee, Bombus terrestris, and an insect predator of WFT, the pirate bug Orius laevigatus. Our data indicate that whereas BFo1 could establish itself in both these non-natural hosts, albeit with no significant effects on insect fitness, BFo2 was unable to persist in either species. In terms of biosafety, these data, together with its more specific growth requirements, vindicate the choice of BFo2 for delivery of RNAi and precision pest management of WFT.
published_date 2023-03-08T10:33:59Z
_version_ 1787518034264457216
score 11.013148

Similar Items