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Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium / MARTYN ALLEN

Swansea University Author: MARTYN ALLEN

  • E-Thesis – open access under embargo until: 14th December 2027

DOI (Published version): 10.23889/SUthesis.62296

Abstract

As defined by the WHO, probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host (WHO, 2002). Commonly, the target site for probiotic bacteria is the gastrointestinal system, and in particular, the epithelial layer of the large and small intes...

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Published: Swansea 2022
Institution: Swansea University
Degree level: Doctoral
Degree name: EngD
Supervisor: Li, Chenfeng ; Dettmer, Wulf ;
URI: https://cronfa.swan.ac.uk/Record/cronfa62296
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first_indexed 2023-01-10T15:34:31Z
last_indexed 2023-01-16T17:31:05Z
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Previously, probiotics and other beneficial organisms were commonly delivered through liquid and semi-solid (i.e. yogurt, milk and other dairy) products - however, these have been shown to lack temporal stability and consumer acceptability. More recently, lyophilisation techniques have allowed freeze-dried probiotics to be included into solid dosage formats that include directly compressed tablets. Probiotic bacteria are often selected based on their ability to exert [often strain-specific] effects on the host - the functionality of which is often assessed as the native strain. Recent work has shown that the influence of exogenous stressors introduced during stages of manufacturing have the ability to affect both viability and functionality, which constitutes an economic burden on the manufacturers of probiotic supplements who need to guarantee both viable number and functionality. Using both culture-dependant and independent techniques, this thesis shows that the manufacturing processes of tabletting induces a loss of viability of the Lab4&#xAE; probiotic consortium in a control formulation. However, using in vitro and cell-based models, this piece of work has shown that the tabletting manufacturing processes does not affect some of the desirable functionalities of the probiotic when compared to the un-processed, control formulation. Pressure-dependant studies subsequently highlighted the causation between tablet compaction pressure and bacterial viability. Following this, optimisation work to improve tabletability and reduce the required compaction pressure was undertaken utilising both machining and formulation parameters. Results showed that optimisation of the control formulation and machining parameters were able to elicit greater bacterial recovery values without compromising on tablet integrity. The results from this Eng.D. can be seen as highly pertinent to the successful design and production of probiotic solid dosage formats, especially tablets. The results from which highlight the impact of tablet manufacturing methods on probiotic viability and functionality, therefore can be utilised to improve the probiotic product whilst also reducing costs through a reduced need to have large overages in bacterial additions to account for the previous losses that occur during tabletting.</abstract><type>E-Thesis</type><journal/><volume/><journalNumber/><paginationStart/><paginationEnd/><publisher/><placeOfPublication>Swansea</placeOfPublication><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic/><keywords>probiotic; tabletting; nutraceuticals; oral solid dose; functionality; viability; bacteria</keywords><publishedDay>14</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-12-14</publishedDate><doi>10.23889/SUthesis.62296</doi><url/><notes/><college>COLLEGE NANME</college><CollegeCode>COLLEGE CODE</CollegeCode><institution>Swansea University</institution><supervisor>Li, Chenfeng ; Dettmer, Wulf ;</supervisor><degreelevel>Doctoral</degreelevel><degreename>EngD</degreename><degreesponsorsfunders>EPSRC/M2A</degreesponsorsfunders><apcterm/><funders/><projectreference/><lastEdited>2023-01-16T15:18:18.8083500</lastEdited><Created>2023-01-10T15:28:10.8991438</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Uncategorised</level></path><authors><author><firstname>MARTYN</firstname><surname>ALLEN</surname><order>1</order></author></authors><documents><document><filename>Under embargo</filename><originalFilename>Under embargo</originalFilename><uploaded>2023-01-10T16:00:03.5594958</uploaded><type>Output</type><contentLength>4447363</contentLength><contentType>application/pdf</contentType><version>E-Thesis &#x2013; open access</version><cronfaStatus>true</cronfaStatus><embargoDate>2027-12-14T00:00:00.0000000</embargoDate><documentNotes>Copyright: The author, Martyn Allen, 2022.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2023-01-16T15:18:18.8083500 v2 62296 2023-01-10 Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium 86a74c6b6ecbd4f453ac26a6f53c77d6 MARTYN ALLEN MARTYN ALLEN true false 2023-01-10 As defined by the WHO, probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host (WHO, 2002). Commonly, the target site for probiotic bacteria is the gastrointestinal system, and in particular, the epithelial layer of the large and small intestines. The oral delivery route is often preferred as a less-invasive, low cost and high patient compliant method. Previously, probiotics and other beneficial organisms were commonly delivered through liquid and semi-solid (i.e. yogurt, milk and other dairy) products - however, these have been shown to lack temporal stability and consumer acceptability. More recently, lyophilisation techniques have allowed freeze-dried probiotics to be included into solid dosage formats that include directly compressed tablets. Probiotic bacteria are often selected based on their ability to exert [often strain-specific] effects on the host - the functionality of which is often assessed as the native strain. Recent work has shown that the influence of exogenous stressors introduced during stages of manufacturing have the ability to affect both viability and functionality, which constitutes an economic burden on the manufacturers of probiotic supplements who need to guarantee both viable number and functionality. Using both culture-dependant and independent techniques, this thesis shows that the manufacturing processes of tabletting induces a loss of viability of the Lab4® probiotic consortium in a control formulation. However, using in vitro and cell-based models, this piece of work has shown that the tabletting manufacturing processes does not affect some of the desirable functionalities of the probiotic when compared to the un-processed, control formulation. Pressure-dependant studies subsequently highlighted the causation between tablet compaction pressure and bacterial viability. Following this, optimisation work to improve tabletability and reduce the required compaction pressure was undertaken utilising both machining and formulation parameters. Results showed that optimisation of the control formulation and machining parameters were able to elicit greater bacterial recovery values without compromising on tablet integrity. The results from this Eng.D. can be seen as highly pertinent to the successful design and production of probiotic solid dosage formats, especially tablets. The results from which highlight the impact of tablet manufacturing methods on probiotic viability and functionality, therefore can be utilised to improve the probiotic product whilst also reducing costs through a reduced need to have large overages in bacterial additions to account for the previous losses that occur during tabletting. E-Thesis Swansea probiotic; tabletting; nutraceuticals; oral solid dose; functionality; viability; bacteria 14 12 2022 2022-12-14 10.23889/SUthesis.62296 COLLEGE NANME COLLEGE CODE Swansea University Li, Chenfeng ; Dettmer, Wulf ; Doctoral EngD EPSRC/M2A 2023-01-16T15:18:18.8083500 2023-01-10T15:28:10.8991438 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised MARTYN ALLEN 1 Under embargo Under embargo 2023-01-10T16:00:03.5594958 Output 4447363 application/pdf E-Thesis – open access true 2027-12-14T00:00:00.0000000 Copyright: The author, Martyn Allen, 2022. true eng
title Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
spellingShingle Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
MARTYN ALLEN
title_short Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
title_full Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
title_fullStr Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
title_full_unstemmed Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
title_sort Assessing the impact of tablet manufacturing on the viability and functionality of the LAB4® probiotic consortium
author_id_str_mv 86a74c6b6ecbd4f453ac26a6f53c77d6
author_id_fullname_str_mv 86a74c6b6ecbd4f453ac26a6f53c77d6_***_MARTYN ALLEN
author MARTYN ALLEN
author2 MARTYN ALLEN
format E-Thesis
publishDate 2022
institution Swansea University
doi_str_mv 10.23889/SUthesis.62296
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 0
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description As defined by the WHO, probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host (WHO, 2002). Commonly, the target site for probiotic bacteria is the gastrointestinal system, and in particular, the epithelial layer of the large and small intestines. The oral delivery route is often preferred as a less-invasive, low cost and high patient compliant method. Previously, probiotics and other beneficial organisms were commonly delivered through liquid and semi-solid (i.e. yogurt, milk and other dairy) products - however, these have been shown to lack temporal stability and consumer acceptability. More recently, lyophilisation techniques have allowed freeze-dried probiotics to be included into solid dosage formats that include directly compressed tablets. Probiotic bacteria are often selected based on their ability to exert [often strain-specific] effects on the host - the functionality of which is often assessed as the native strain. Recent work has shown that the influence of exogenous stressors introduced during stages of manufacturing have the ability to affect both viability and functionality, which constitutes an economic burden on the manufacturers of probiotic supplements who need to guarantee both viable number and functionality. Using both culture-dependant and independent techniques, this thesis shows that the manufacturing processes of tabletting induces a loss of viability of the Lab4® probiotic consortium in a control formulation. However, using in vitro and cell-based models, this piece of work has shown that the tabletting manufacturing processes does not affect some of the desirable functionalities of the probiotic when compared to the un-processed, control formulation. Pressure-dependant studies subsequently highlighted the causation between tablet compaction pressure and bacterial viability. Following this, optimisation work to improve tabletability and reduce the required compaction pressure was undertaken utilising both machining and formulation parameters. Results showed that optimisation of the control formulation and machining parameters were able to elicit greater bacterial recovery values without compromising on tablet integrity. The results from this Eng.D. can be seen as highly pertinent to the successful design and production of probiotic solid dosage formats, especially tablets. The results from which highlight the impact of tablet manufacturing methods on probiotic viability and functionality, therefore can be utilised to improve the probiotic product whilst also reducing costs through a reduced need to have large overages in bacterial additions to account for the previous losses that occur during tabletting.
published_date 2022-12-14T04:21:47Z
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