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Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels

Simona Ferraraccio Orcid Logo, Paolo Bertoncello Orcid Logo

Bioelectrochemistry, Volume: 150, Start page: 108365

Swansea University Authors: Simona Ferraraccio Orcid Logo, Paolo Bertoncello Orcid Logo

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DOI (Published version): 10.1016/j.bioelechem.2023.108365

Abstract

Two dehydrogenase enzymes (glucose, GDH, and lactate, LDH, dehydrogenases) encapsulated within alginate hydrogels were deposited on glassy carbon electrodes. The as-prepared enzyme modified alginate hydrogels were utilized as electrochemiluminescence (ECL)-based biosensors for the indirect detection...

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Published in: Bioelectrochemistry
ISSN: 1567-5394
Published: Elsevier BV 2023
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URI: https://cronfa.swan.ac.uk/Record/cronfa62252
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spelling 2023-02-01T18:06:35.5768138 v2 62252 2023-01-03 Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels 1300f1211abc29e989f5aee998831070 0000-0001-6949-6882 Simona Ferraraccio Simona Ferraraccio true false ad352842aa5fe9c1947bd24ff61816c8 0000-0002-6557-7885 Paolo Bertoncello Paolo Bertoncello true false 2023-01-03 CHEG Two dehydrogenase enzymes (glucose, GDH, and lactate, LDH, dehydrogenases) encapsulated within alginate hydrogels were deposited on glassy carbon electrodes. The as-prepared enzyme modified alginate hydrogels were utilized as electrochemiluminescence (ECL)-based biosensors for the indirect detection of glucose and lactic acid upon reaction between NADH and tris(2,2′-bipyridyl) ruthenium (II) [Ru(bpy)3]2+. The ECL response was obtained from the redox reaction between the substrate, the cofactor NAD+ and the encapsulated enzyme. The production of NADH resulting from the enzymatic reaction led to the ECL emission upon reaction with [Ru(bpy)3]2+. The biosensors showed good stability and repeatability, with linear range between 0.56-4.2 µM and limit of detection of 0.84 µM for glucose, and linear range between 5-30 µM with a limit of detection of 2.52 µM for lactic acid. These ECL-based biosensors showed good sensitivity when tested in the presence of common interfering species. These biosensors were utilized in artificial sweat and were characterized by good reproducibility and repeatability. The results herein presented suggest that the dehydrogenases encapsulated within alginate hydrogels have potential for the development of biocompatible sensors for detection of glucose and lactic acid in physiological fluids. Journal Article Bioelectrochemistry 150 108365 Elsevier BV 1567-5394 Enzymes; Electrochemiluminescence; Bioelectrochemistry; Biosensors; Glucose dehydrogenase; Lactate dehydrogenase 1 4 2023 2023-04-01 10.1016/j.bioelechem.2023.108365 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University SU Library paid the OA fee (TA Institutional Deal) Welsh Government, Swansea University 2023-02-01T18:06:35.5768138 2023-01-03T13:43:49.2892449 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Simona Ferraraccio 0000-0001-6949-6882 1 Paolo Bertoncello 0000-0002-6557-7885 2 62252__26273__15dcfaac9c3b4267b8f1da9017339893.pdf 62252 (2).pdf 2023-01-12T15:07:16.3742132 Output 3295446 application/pdf Version of Record true © 2023 The Author(s). This is an open access article under the CC BY license true eng https://creativecommons.org/licenses/by/4.0/
title Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
spellingShingle Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
Simona Ferraraccio
Paolo Bertoncello
title_short Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
title_full Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
title_fullStr Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
title_full_unstemmed Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
title_sort Electrochemiluminescence (ECL) biosensor based on tris(2,2′-bipyridyl)ruthenium(II) with glucose and lactate dehydrogenases encapsulated within alginate hydrogels
author_id_str_mv 1300f1211abc29e989f5aee998831070
ad352842aa5fe9c1947bd24ff61816c8
author_id_fullname_str_mv 1300f1211abc29e989f5aee998831070_***_Simona Ferraraccio
ad352842aa5fe9c1947bd24ff61816c8_***_Paolo Bertoncello
author Simona Ferraraccio
Paolo Bertoncello
author2 Simona Ferraraccio
Paolo Bertoncello
format Journal article
container_title Bioelectrochemistry
container_volume 150
container_start_page 108365
publishDate 2023
institution Swansea University
issn 1567-5394
doi_str_mv 10.1016/j.bioelechem.2023.108365
publisher Elsevier BV
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 1
active_str 0
description Two dehydrogenase enzymes (glucose, GDH, and lactate, LDH, dehydrogenases) encapsulated within alginate hydrogels were deposited on glassy carbon electrodes. The as-prepared enzyme modified alginate hydrogels were utilized as electrochemiluminescence (ECL)-based biosensors for the indirect detection of glucose and lactic acid upon reaction between NADH and tris(2,2′-bipyridyl) ruthenium (II) [Ru(bpy)3]2+. The ECL response was obtained from the redox reaction between the substrate, the cofactor NAD+ and the encapsulated enzyme. The production of NADH resulting from the enzymatic reaction led to the ECL emission upon reaction with [Ru(bpy)3]2+. The biosensors showed good stability and repeatability, with linear range between 0.56-4.2 µM and limit of detection of 0.84 µM for glucose, and linear range between 5-30 µM with a limit of detection of 2.52 µM for lactic acid. These ECL-based biosensors showed good sensitivity when tested in the presence of common interfering species. These biosensors were utilized in artificial sweat and were characterized by good reproducibility and repeatability. The results herein presented suggest that the dehydrogenases encapsulated within alginate hydrogels have potential for the development of biocompatible sensors for detection of glucose and lactic acid in physiological fluids.
published_date 2023-04-01T04:21:43Z
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