Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Martínez-Rodríguez, Pablo; Sánchez-Castro, Iván; Ledo, Jesus Ojeda; Abad, María M.; Descostes, Michael; Merroun, Mohamed Larbi

doi:10.3389/fmicb.2022.1092184

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Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

Pablo Martínez-Rodríguez, Iván Sánchez-Castro, Jesus Ojeda Ledo

, María M. Abad, Michael Descostes, Mohamed Larbi Merroun

Frontiers in Microbiology, Volume: 13

Swansea University Author: Jesus Ojeda Ledo

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DOI (Published version): 10.3389/fmicb.2022.1092184

Abstract

Industrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-...

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Published in:	Frontiers in Microbiology
ISSN:	1664-302X
Published:	Frontiers Media SA 2023
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Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g. phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unravelled to optimize results when applying this remediation process. In this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.). It was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. 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spelling	2023-01-11T16:57:02.9111542 v2 62183 2022-12-16 Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2022-12-16 CHEG Industrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g. phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unravelled to optimize results when applying this remediation process. In this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.). It was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation. Journal Article Frontiers in Microbiology 13 Frontiers Media SA 1664-302X Microbacterium, Uranium, Phosphate source, Bioprecipitation, Solubilization, PSB 9 1 2023 2023-01-09 10.3389/fmicb.2022.1092184 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University Another institution paid the OA fee This work was supported by ORANO Mining (France; collaborative research contract no 3022 OTRI-UGR). It results from a Joint Research Project between Orano Mining R&D Department and the Department of Microbiology of the University of Granada 2023-01-11T16:57:02.9111542 2022-12-16T16:46:27.1153741 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Pablo Martínez-Rodríguez 1 Iván Sánchez-Castro 2 Jesus Ojeda Ledo 0000-0002-2046-1010 3 María M. Abad 4 Michael Descostes 5 Mohamed Larbi Merroun 6 62183__26263__5f1e5b741ebf4cf38e65c5f309ecf3ff.pdf 62183.pdf 2023-01-11T16:55:06.2705632 Output 2447858 application/pdf Version of Record true © 2023 Martínez-Rodríguez, SánchezCastro, Ojeda, Abad, Descostes and Merroun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng http://creativecommons.org/licenses/by/4.0/
title	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
spellingShingle	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study Jesus Ojeda Ledo
title_short	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
title_full	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
title_fullStr	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
title_full_unstemmed	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
title_sort	Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study
author_id_str_mv	4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv	4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author	Jesus Ojeda Ledo
author2	Pablo Martínez-Rodríguez Iván Sánchez-Castro Jesus Ojeda Ledo María M. Abad Michael Descostes Mohamed Larbi Merroun
format	Journal article
container_title	Frontiers in Microbiology
container_volume	13
publishDate	2023
institution	Swansea University
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doi_str_mv	10.3389/fmicb.2022.1092184
publisher	Frontiers Media SA
college_str	Faculty of Science and Engineering
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hierarchy_top_title	Faculty of Science and Engineering
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department_str	School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
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description	Industrial activities related with the uranium industry are known to generate hazardous waste which must be managed adequately. Amongst the remediation activities available, eco-friendly strategies based on microbial activity have been investigated in depth in the last decades and biomineralization-based methods, mediated by microbial enzymes (e.g. phosphatase), have been proposed as a promising approach. However, the presence of different forms of phosphates in these environments plays a complicated role which must be thoroughly unravelled to optimize results when applying this remediation process. In this study, we have looked at the effect of different phosphate sources on the uranium (U) biomineralization process mediated by Microbacterium sp. Be9, a bacterial strain previously isolated from U mill tailings. We applied a multidisciplinary approach (cell surface characterization, phosphatase activity, inorganic phosphate release, cell viability, microscopy, etc.). It was clear that the U removal ability and related U interaction mechanisms by the strain depend on the type of phosphate substrate. In the absence of exogenous phosphate substrate, the cells interact with U through U phosphate biomineralization with a 98% removal of U within the first 48h. However, the U solubilization process was the main U interaction mechanism of the cells in the presence of inorganic phosphate, demonstrating the phosphate solubilizing potential of the strain. These findings show the biotechnological use of this strain in the bioremediation of U as a function of phosphate substrate: U biomineralization (in a phosphate free system) and indirectly through the solubilization of orthophosphate from phosphate (P) containing waste products needed for U precipitation.
published_date	2023-01-09T04:21:36Z
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Effect of different phosphate sources on uranium biomineralization by the Microbacterium sp. Be9 strain: A multidisciplinary approach study

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