Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications

Ferraraccio, Simona; Lisa, Donatella Di; Pastorino, Laura; Bertoncello, Paolo

doi:10.1021/acs.analchem.2c03389

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Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications

Simona Ferraraccio, Donatella Di Lisa, Laura Pastorino

, Paolo Bertoncello

Analytical Chemistry, Volume: 94, Issue: 46, Pages: 16122 - 16131

Swansea University Authors: Simona Ferraraccio, Paolo Bertoncello

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DOI (Published version): 10.1021/acs.analchem.2c03389

Abstract

A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into...

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Published in:	Analytical Chemistry
ISSN:	0003-2700 1520-6882
Published:	American Chemical Society (ACS) 2022
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa61826

first_indexed	2022-11-09T10:03:55Z
last_indexed	2023-01-13T19:22:51Z
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spelling	2023-01-05T16:12:39.8372153 v2 61826 2022-11-09 Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications 1300f1211abc29e989f5aee998831070 Simona Ferraraccio Simona Ferraraccio true false ad352842aa5fe9c1947bd24ff61816c8 0000-0002-6557-7885 Paolo Bertoncello Paolo Bertoncello true false 2022-11-09 A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis–Menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μM, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μM for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples. Journal Article Analytical Chemistry 94 46 16122 16131 American Chemical Society (ACS) 0003-2700 1520-6882 22 11 2022 2022-11-22 10.1021/acs.analchem.2c03389 COLLEGE NANME COLLEGE CODE Swansea University SU Library paid the OA fee (TA Institutional Deal) L.S.F. gratefully acknowledges financial support from the Knowledge Economy Skills PhD Scholarship (KESS2) under the Welsh Government’s European Social Fund (ESF) convergence program for West Wales and the Valleys and Perpetuus Carbon Ltd. 2023-01-05T16:12:39.8372153 2022-11-09T09:33:24.2408993 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Simona Ferraraccio 1 Donatella Di Lisa 2 Laura Pastorino 0000-0002-5928-3856 3 Paolo Bertoncello 0000-0002-6557-7885 4 61826__25704__05fc816d00164ea4a92d6d95f4e7a9e4.pdf 61826_VoR.pdf 2022-11-09T10:04:38.0547047 Output 6761367 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY 4.0) true eng https://creativecommons.org/licenses/by/4.0/
title	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
spellingShingle	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications Simona Ferraraccio Paolo Bertoncello
title_short	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_full	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_fullStr	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_full_unstemmed	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
title_sort	Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications
author_id_str_mv	1300f1211abc29e989f5aee998831070 ad352842aa5fe9c1947bd24ff61816c8
author_id_fullname_str_mv	1300f1211abc29e989f5aee998831070_*_Simona Ferraraccio ad352842aa5fe9c1947bd24ff61816c8_*_Paolo Bertoncello
author	Simona Ferraraccio Paolo Bertoncello
author2	Simona Ferraraccio Donatella Di Lisa Laura Pastorino Paolo Bertoncello
format	Journal article
container_title	Analytical Chemistry
container_volume	94
container_issue	46
container_start_page	16122
publishDate	2022
institution	Swansea University
issn	0003-2700 1520-6882
doi_str_mv	10.1021/acs.analchem.2c03389
publisher	American Chemical Society (ACS)
college_str	Faculty of Science and Engineering
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hierarchy_top_title	Faculty of Science and Engineering
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department_str	School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
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description	A simple procedure to incorporate enzymes (horseradish peroxidase, HRP, and lactate oxidase, LOx) within alginate hydrogels is reported with electrochemiluminescence (ECL) used to detect the enzymatic reactions with the corresponding substrates. First, HRP and LOx were successfully immobilized into CaCO3 microspheres, followed by the electrostatic layer-by-layer deposition of a nanoshell onto the microspheres, and finally by their dispersion into alginate solution. The as-prepared dispersion was drop cast onto the glassy carbon electrodes and cross-linked by the external and internal gelation methods using Ca2+ cations. The enzymes encapsulated within the alginate hydrogels were characterized using cyclic voltammetry and kinetic studies performed using ECL. The results showed that the enzymatic activity was significantly maintained as a result of the immobilization, with values of the apparent Michaelis–Menten constants estimated as 7.71 ± 0.62 and 8.41 ± 0.43 μM, for HRP and LOx, respectively. The proposed biosensors showed good stability and repeatability with an estimated limit of detection of 5.38 ± 0.05 and 0.50 ± 0.03 μM for hydrogen peroxide and lactic acid, respectively. The as-prepared enzymes encapsulated within the alginate hydrogels showed good stability up to 28 days from their preparation. The sensitivity and selectivity of the enzymes encapsulated within the alginate hydrogels were tested in real matrices (HRP, hydrogen peroxide, in contact lens solution; LOx, lactic acid in artificial sweat) showing the sensitivity of the ECL detection methods for the detection of hydrogen peroxide and lactic acid in real samples.
published_date	2022-11-22T08:16:42Z
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score	11.047739

Enzymes Encapsulated within Alginate Hydrogels: Bioelectrocatalysis and Electrochemiluminescence Applications

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