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Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association / XIAORUI CHEN

Swansea University Author: XIAORUI CHEN

DOI (Published version): 10.23889/SUthesis.61749

Abstract

Pest control in agriculture employs diverse strategies, among which the use of predatory insects has steadily increased. The use of several species within the genus Orius in pest control is widely spread, particularly in Mediterranean Europe. The use of predatory insects in pest control in agricultu...

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Published: Swansea 2022
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
Supervisor: Del Sol, Ricardo
URI: https://cronfa.swan.ac.uk/Record/cronfa61749
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first_indexed 2022-11-01T11:48:13Z
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spelling 2022-11-01T12:22:34.1830621 v2 61749 2022-11-01 Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association fcd35489adbc51f1df9df0379c5f25ec XIAORUI CHEN XIAORUI CHEN true false 2022-11-01 Pest control in agriculture employs diverse strategies, among which the use of predatory insects has steadily increased. The use of several species within the genus Orius in pest control is widely spread, particularly in Mediterranean Europe. The use of predatory insects in pest control in agriculture has spread worldwide and increased significantly, especially in the use of various Orius species. Currently, most studies about Orius species have been focused on the diet manipulation or selective breeding methods to reduce the rearing costs and improve the efficiency, only a few studies were associated to their Wolbachia symbionts. The characterisation and contribution of microbial symbionts to Orius sp. fitness, behaviour, and potential impact on human health has been neglected. Therefore, there is a lack of knowledge regarding Orius’ symbionts such as their taxonomic characterisation, the functions of the symbionts and potential influences on human health. This project was focused on the first comparative genomics report of genome sequences level description of the predominant culturable facultative bacterial symbionts associated with the analyses of draft genomes of facultative symbionts using Next Generation Sequencing (NGS) technique related to five Orius species (Orius laevigatus, Orius niger, Orius pallidicornis, Orius majusculus and Orius albidipennis) and collected from various European countries (Greece, Italy, and Spain). Initially, coxl (COI) based taxonomic classification of the Orius species used was performed, followed by the isolation of culturable bacteria from live insects. The whole genome sequences of the bacterial isolates were generated and assembled into draft genomes using NGS. The isolates of two predominant bacteria belong to Serratia and Leucobacter genera, the third predominant bacteria are most likely to be a new genus within the Erwiniaceae. Orius sp. Serratia isolates genomes are more similar to Serratia sp. SCBI. Pan-genome analysis of Serratia sp. Orius isolates evidenced an open pan-genome, and 279 accessory genes were related to the insect symbiosis trait. Additionally, pan-genome analyses of the Serratia sp. isolates offered clues linking Type VI secretion system effector–immunity proteins from the Tai4 sub-family to the symbiotic lifestyle. These symbionts were found to colonise all the insect specimens tested, which evidenced an ancestral symbiotic association between these bacteria and the genus Orius. Additionally, plasmid sequence analyses suggest sequence exchanges between Serratia sp. Orius isolates and pathogenic Serratia species, which may have implications for food safety and human health. E-Thesis Swansea Microbiology, bioinformatic, insect symbiont 26 10 2022 2022-10-26 10.23889/SUthesis.61749 COLLEGE NANME COLLEGE CODE Swansea University Del Sol, Ricardo Doctoral Ph.D 2022-11-01T12:22:34.1830621 2022-11-01T11:45:28.9880130 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine XIAORUI CHEN 1 61749__25623__8ac4f49401b5436aa0a77a7cc555b260.pdf Chen_Xiaorui_PhD_Thesis_Final_Redacted_Signature.pdf 2022-11-01T12:11:08.8095123 Output 21847979 application/pdf E-Thesis – open access true Copyright: The author, Xiaorui Chen, 2022. true eng
title Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
spellingShingle Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
XIAORUI CHEN
title_short Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
title_full Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
title_fullStr Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
title_full_unstemmed Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
title_sort Facultative bacterial symbionts from European Orius species: Evidence for an ancestral symbiotic association
author_id_str_mv fcd35489adbc51f1df9df0379c5f25ec
author_id_fullname_str_mv fcd35489adbc51f1df9df0379c5f25ec_***_XIAORUI CHEN
author XIAORUI CHEN
author2 XIAORUI CHEN
format E-Thesis
publishDate 2022
institution Swansea University
doi_str_mv 10.23889/SUthesis.61749
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Pest control in agriculture employs diverse strategies, among which the use of predatory insects has steadily increased. The use of several species within the genus Orius in pest control is widely spread, particularly in Mediterranean Europe. The use of predatory insects in pest control in agriculture has spread worldwide and increased significantly, especially in the use of various Orius species. Currently, most studies about Orius species have been focused on the diet manipulation or selective breeding methods to reduce the rearing costs and improve the efficiency, only a few studies were associated to their Wolbachia symbionts. The characterisation and contribution of microbial symbionts to Orius sp. fitness, behaviour, and potential impact on human health has been neglected. Therefore, there is a lack of knowledge regarding Orius’ symbionts such as their taxonomic characterisation, the functions of the symbionts and potential influences on human health. This project was focused on the first comparative genomics report of genome sequences level description of the predominant culturable facultative bacterial symbionts associated with the analyses of draft genomes of facultative symbionts using Next Generation Sequencing (NGS) technique related to five Orius species (Orius laevigatus, Orius niger, Orius pallidicornis, Orius majusculus and Orius albidipennis) and collected from various European countries (Greece, Italy, and Spain). Initially, coxl (COI) based taxonomic classification of the Orius species used was performed, followed by the isolation of culturable bacteria from live insects. The whole genome sequences of the bacterial isolates were generated and assembled into draft genomes using NGS. The isolates of two predominant bacteria belong to Serratia and Leucobacter genera, the third predominant bacteria are most likely to be a new genus within the Erwiniaceae. Orius sp. Serratia isolates genomes are more similar to Serratia sp. SCBI. Pan-genome analysis of Serratia sp. Orius isolates evidenced an open pan-genome, and 279 accessory genes were related to the insect symbiosis trait. Additionally, pan-genome analyses of the Serratia sp. isolates offered clues linking Type VI secretion system effector–immunity proteins from the Tai4 sub-family to the symbiotic lifestyle. These symbionts were found to colonise all the insect specimens tested, which evidenced an ancestral symbiotic association between these bacteria and the genus Orius. Additionally, plasmid sequence analyses suggest sequence exchanges between Serratia sp. Orius isolates and pathogenic Serratia species, which may have implications for food safety and human health.
published_date 2022-10-26T04:20:48Z
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