Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.

Paulsen, Sara Skøtt; Isbrandt, Thomas; Kirkegaard, Markus; Buijs, Yannick; Strube, Mikael Lenz; Sonnenschein, Eva C.; Larsen, Thomas O.; Gram, Lone

doi:10.1038/s41598-020-78439-3

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Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.

Sara Skøtt Paulsen, Thomas Isbrandt, Markus Kirkegaard, Yannick Buijs, Mikael Lenz Strube, Eva C. Sonnenschein

, Thomas O. Larsen, Lone Gram

Scientific Reports, Volume: 10, Issue: 1

Swansea University Author: Eva C. Sonnenschein

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DOI (Published version): 10.1038/s41598-020-78439-3

Abstract

Novel antimicrobials are urgently needed due to the rapid spread of antibiotic resistant bacteria. In a genome-wide analysis of Pseudoalteromonas strains, one strain (S4498) was noticed due to its potent antibiotic activity. It did not produce the yellow antimicrobial pigment bromoalterochromide, wh...

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Published in:	Scientific Reports
ISSN:	2045-2322
Published:	Springer Science and Business Media LLC 2020
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spelling	2022-11-04T17:32:13.2156248 v2 61722 2022-10-31 Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov. f6a4027578a15ea3e6453a54b849c686 0000-0001-6959-5100 Eva C. Sonnenschein Eva C. Sonnenschein true false 2022-10-31 SBI Novel antimicrobials are urgently needed due to the rapid spread of antibiotic resistant bacteria. In a genome-wide analysis of Pseudoalteromonas strains, one strain (S4498) was noticed due to its potent antibiotic activity. It did not produce the yellow antimicrobial pigment bromoalterochromide, which was produced by several related type strains with which it shared less than 95% average nucleotide identity. Also, it produced a sweet-smelling volatile not observed from other strains. Mining the genome of strain S4498 using the secondary metabolite prediction tool antiSMASH led to eight biosynthetic gene clusters with no homology to known compounds, and synteny analyses revealed that the yellow pigment bromoalterochromide was likely lost during evolution. Metabolome profiling of strain S4498 using HPLC-HRMS analyses revealed marked differences to the type strains. In particular, a series of quinolones known as pseudanes were identified and verified by NMR. The characteristic odor of the strain was linked to the pseudanes. The highly halogenated compound tetrabromopyrrole was detected as the major antibacterial component by bioassay-guided fractionation. Taken together, the polyphasic analysis demonstrates that strain S4498 belongs to a novel species within the genus Pseudoalteromonas, and we propose the name Pseudoalteromonas galatheae sp. nov. (type strain S4498T = NCIMB 15250T = LMG 31599T). Journal Article Scientific Reports 10 1 Springer Science and Business Media LLC 2045-2322 10 12 2020 2020-12-10 10.1038/s41598-020-78439-3 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University The study was funded by the Villum Foundation (Annual Award 2016 to LG), The Danish National Research Foundation (DNRF137) and Independent Research Fund Denmark (FTP). 2022-11-04T17:32:13.2156248 2022-10-31T12:58:30.2216662 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Sara Skøtt Paulsen 1 Thomas Isbrandt 2 Markus Kirkegaard 3 Yannick Buijs 4 Mikael Lenz Strube 5 Eva C. Sonnenschein 0000-0001-6959-5100 6 Thomas O. Larsen 7 Lone Gram 8 61722__25656__32121fc4565040be9b9ab7b91135e992.pdf 61722.pdf 2022-11-04T17:30:39.9450905 Output 5112258 application/pdf Version of Record true © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
spellingShingle	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov. Eva C. Sonnenschein
title_short	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
title_full	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
title_fullStr	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
title_full_unstemmed	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
title_sort	Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.
author_id_str_mv	f6a4027578a15ea3e6453a54b849c686
author_id_fullname_str_mv	f6a4027578a15ea3e6453a54b849c686_***_Eva C. Sonnenschein
author	Eva C. Sonnenschein
author2	Sara Skøtt Paulsen Thomas Isbrandt Markus Kirkegaard Yannick Buijs Mikael Lenz Strube Eva C. Sonnenschein Thomas O. Larsen Lone Gram
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container_title	Scientific Reports
container_volume	10
container_issue	1
publishDate	2020
institution	Swansea University
issn	2045-2322
doi_str_mv	10.1038/s41598-020-78439-3
publisher	Springer Science and Business Media LLC
college_str	Faculty of Science and Engineering
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hierarchy_parent_title	Faculty of Science and Engineering
department_str	School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
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description	Novel antimicrobials are urgently needed due to the rapid spread of antibiotic resistant bacteria. In a genome-wide analysis of Pseudoalteromonas strains, one strain (S4498) was noticed due to its potent antibiotic activity. It did not produce the yellow antimicrobial pigment bromoalterochromide, which was produced by several related type strains with which it shared less than 95% average nucleotide identity. Also, it produced a sweet-smelling volatile not observed from other strains. Mining the genome of strain S4498 using the secondary metabolite prediction tool antiSMASH led to eight biosynthetic gene clusters with no homology to known compounds, and synteny analyses revealed that the yellow pigment bromoalterochromide was likely lost during evolution. Metabolome profiling of strain S4498 using HPLC-HRMS analyses revealed marked differences to the type strains. In particular, a series of quinolones known as pseudanes were identified and verified by NMR. The characteristic odor of the strain was linked to the pseudanes. The highly halogenated compound tetrabromopyrrole was detected as the major antibacterial component by bioassay-guided fractionation. Taken together, the polyphasic analysis demonstrates that strain S4498 belongs to a novel species within the genus Pseudoalteromonas, and we propose the name Pseudoalteromonas galatheae sp. nov. (type strain S4498T = NCIMB 15250T = LMG 31599T).
published_date	2020-12-10T04:20:45Z
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score	11.013507

Production of the antimicrobial compound tetrabromopyrrole and the Pseudomonas quinolone system precursor, 2-heptyl-4-quinolone, by a novel marine species Pseudoalteromonas galatheae sp. nov.

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