Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Dickson, Alison; Yutuc, Eylan; Thornton, Cathy; Wang, Yuqin; Griffiths, William

doi:10.3389/fendo.2022.1031013

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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Alison Dickson, Eylan Yutuc

, Cathy Thornton

, Yuqin Wang

, William Griffiths

Frontiers in Endocrinology, Volume: 13

Swansea University Authors: Alison Dickson, Eylan Yutuc , Cathy Thornton , Yuqin Wang , William Griffiths

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DOI (Published version): 10.3389/fendo.2022.1031013

Abstract

The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human...

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Published in:	Frontiers in Endocrinology
ISSN:	1664-2392
Published:	Frontiers Media SA 2022
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URI:	https://cronfa.swan.ac.uk/Record/cronfa61630
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first_indexed	2022-10-28T12:00:11Z
last_indexed	2023-01-13T19:22:30Z
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We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. 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spelling	v2 61630 2022-10-21 Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry 82dc4360769527a5f3a0cd9d85cdff40 Alison Dickson Alison Dickson true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2022-10-21 BMS The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women. Journal Article Frontiers in Endocrinology 13 Frontiers Media SA 1664-2392 sterol, oxysterol, bile acids, CYP11A1, LC-MS, CYP27A1 10 11 2022 2022-11-10 10.3389/fendo.2022.1031013 Research data via DOI 10.17605/OSF.IO/EGNCZ COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WJG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WJG and YW). ALD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund. 2023-09-13T15:35:38.7538528 2022-10-21T09:49:11.5942068 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Alison Dickson 1 Eylan Yutuc 0000-0001-9971-1950 2 Cathy Thornton 0000-0002-5153-573X 3 Yuqin Wang 0000-0002-3063-3066 4 William Griffiths 0000-0002-4129-6616 5 61630__25756__6a2ada534d9a4386911ef8cb0cb259ad.pdf 61630.pdf 2022-11-14T11:25:27.5744096 Output 14659915 application/pdf Version of Record true © 2022 Dickson, Yutuc, Thornton, Wang and Griffiths. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY) true eng http://creativecommons.org/licenses/by/4.0/
title	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
spellingShingle	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry Alison Dickson Eylan Yutuc Cathy Thornton Yuqin Wang William Griffiths
title_short	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_full	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_fullStr	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_full_unstemmed	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_sort	Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
author_id_str_mv	82dc4360769527a5f3a0cd9d85cdff40 99332f073ce913a9b7d8b6441b17516d c71a7a4be7361094d046d312202bce0c c92729b58622f9fdf6a0e7d8f4ce5081 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv	82dc4360769527a5f3a0cd9d85cdff40_*_Alison Dickson 99332f073ce913a9b7d8b6441b17516d__Eylan Yutuc c71a7a4be7361094d046d312202bce0c__Cathy Thornton c92729b58622f9fdf6a0e7d8f4ce5081__Yuqin Wang 3316b1d1b524be1831790933eed1c26e_**_William Griffiths
author	Alison Dickson Eylan Yutuc Cathy Thornton Yuqin Wang William Griffiths
author2	Alison Dickson Eylan Yutuc Cathy Thornton Yuqin Wang William Griffiths
format	Journal article
container_title	Frontiers in Endocrinology
container_volume	13
publishDate	2022
institution	Swansea University
issn	1664-2392
doi_str_mv	10.3389/fendo.2022.1031013
publisher	Frontiers Media SA
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description	The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
published_date	2022-11-10T15:35:40Z
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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

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