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Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry

Alison Dickson, Eylan Yutuc Orcid Logo, Cathy Thornton Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

Frontiers in Endocrinology, Volume: 13

Swansea University Authors: Alison Dickson, Eylan Yutuc Orcid Logo, Cathy Thornton Orcid Logo, Yuqin Wang Orcid Logo, William Griffiths Orcid Logo

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DOI (Published version): 10.3389/fendo.2022.1031013

Abstract

The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human...

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Published in: Frontiers in Endocrinology
ISSN: 1664-2392
Published: Frontiers Media SA 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61630
first_indexed 2022-10-28T12:00:11Z
last_indexed 2024-11-14T12:19:26Z
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spelling 2023-09-13T15:35:38.7538528 v2 61630 2022-10-21 Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry 82dc4360769527a5f3a0cd9d85cdff40 Alison Dickson Alison Dickson true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2022-10-21 MEDS The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women. Journal Article Frontiers in Endocrinology 13 Frontiers Media SA 1664-2392 sterol, oxysterol, bile acids, CYP11A1, LC-MS, CYP27A1 10 11 2022 2022-11-10 10.3389/fendo.2022.1031013 Research data via DOI 10.17605/OSF.IO/EGNCZ COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC, grant numbers BB/I001735/1, BB/N015932/1 and BB/S019588/1 to WJG, BB/L001942/1 to YW), the European Union through European Structural Funds (ESF), as part of the Welsh Government funded Academic Expertise for Business project (to WJG and YW). ALD was supported via a KESS2 award in association with Markes International from the Welsh Government and the European Social Fund. 2023-09-13T15:35:38.7538528 2022-10-21T09:49:11.5942068 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Alison Dickson 1 Eylan Yutuc 0000-0001-9971-1950 2 Cathy Thornton 0000-0002-5153-573X 3 Yuqin Wang 0000-0002-3063-3066 4 William Griffiths 0000-0002-4129-6616 5 61630__25756__6a2ada534d9a4386911ef8cb0cb259ad.pdf 61630.pdf 2022-11-14T11:25:27.5744096 Output 14659915 application/pdf Version of Record true © 2022 Dickson, Yutuc, Thornton, Wang and Griffiths. This is an openaccess article distributed under the terms of the Creative Commons Attribution License (CC BY) true eng http://creativecommons.org/licenses/by/4.0/
title Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
spellingShingle Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
Alison Dickson
Eylan Yutuc
Cathy Thornton
Yuqin Wang
William Griffiths
title_short Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_full Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_fullStr Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_full_unstemmed Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
title_sort Identification of unusual oxysterols biosynthesised in human pregnancy by charge-tagging and liquid chromatography - mass spectrometry
author_id_str_mv 82dc4360769527a5f3a0cd9d85cdff40
99332f073ce913a9b7d8b6441b17516d
c71a7a4be7361094d046d312202bce0c
c92729b58622f9fdf6a0e7d8f4ce5081
3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 82dc4360769527a5f3a0cd9d85cdff40_***_Alison Dickson
99332f073ce913a9b7d8b6441b17516d_***_Eylan Yutuc
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author Alison Dickson
Eylan Yutuc
Cathy Thornton
Yuqin Wang
William Griffiths
author2 Alison Dickson
Eylan Yutuc
Cathy Thornton
Yuqin Wang
William Griffiths
format Journal article
container_title Frontiers in Endocrinology
container_volume 13
publishDate 2022
institution Swansea University
issn 1664-2392
doi_str_mv 10.3389/fendo.2022.1031013
publisher Frontiers Media SA
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description The aim of this study was to identify oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S-hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3β,20R,22R-trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.
published_date 2022-11-10T20:16:34Z
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