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Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells

Elaine L. Ferguson, Sameza Naseer, Lydia Powell Orcid Logo, Joseph Hardwicke, Fraser I. Young, Bangfu Zhu, Qian Liu Orcid Logo, Bing Song, David W. Thomas

Stem Cell Research, Volume: 33, Pages: 69 - 78

Swansea University Author: Lydia Powell Orcid Logo

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DOI (Published version): 10.1016/j.scr.2018.10.008

Abstract

An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 m...

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Published in: Stem Cell Research
ISSN: 1873-5061
Published: Elsevier BV 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa61618
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spelling 2022-11-09T12:23:11.7656487 v2 61618 2022-10-20 Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells 0e7e702952672bcbfdfd4974199202fb 0000-0002-8641-0160 Lydia Powell Lydia Powell true false 2022-10-20 BMS An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair. Journal Article Stem Cell Research 33 69 78 Elsevier BV 1873-5061 Polymer therapeutics; Biodegradable polymers; Growth factors; Neural stem cells; Controlled release 1 12 2018 2018-12-01 10.1016/j.scr.2018.10.008 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University This work was supported by European Research Council StG grant 243261 (to BS) and Cardiff University. JH was funded by a Fellowship from The Healing Foundation (Registered Charity number 1078666) and the Welsh Office for Research and Development (WORD). 2022-11-09T12:23:11.7656487 2022-10-20T14:35:49.7446925 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Elaine L. Ferguson 1 Sameza Naseer 2 Lydia Powell 0000-0002-8641-0160 3 Joseph Hardwicke 4 Fraser I. Young 5 Bangfu Zhu 6 Qian Liu 0000-0002-1937-0568 7 Bing Song 8 David W. Thomas 9 61618__25709__b2c86f8d65134f83a4c716ced35b4d3f.pdf 61618.pdf 2022-11-09T12:00:37.9862272 Output 1347552 application/pdf Version of Record true © 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/BY/4.0/
title Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
spellingShingle Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
Lydia Powell
title_short Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
title_full Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
title_fullStr Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
title_full_unstemmed Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
title_sort Controlled release of dextrin-conjugated growth factors to support growth and differentiation of neural stem cells
author_id_str_mv 0e7e702952672bcbfdfd4974199202fb
author_id_fullname_str_mv 0e7e702952672bcbfdfd4974199202fb_***_Lydia Powell
author Lydia Powell
author2 Elaine L. Ferguson
Sameza Naseer
Lydia Powell
Joseph Hardwicke
Fraser I. Young
Bangfu Zhu
Qian Liu
Bing Song
David W. Thomas
format Journal article
container_title Stem Cell Research
container_volume 33
container_start_page 69
publishDate 2018
institution Swansea University
issn 1873-5061
doi_str_mv 10.1016/j.scr.2018.10.008
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description An essential aspect of stem cell in vitro culture and in vivo therapy is achieving sustained levels of growth factors to support stem cell survival and expansion, while maintaining their multipotency and differentiation potential. This study investigated the ability of dextrin (~74,000 g/mol; 27.8 mol% succinoylation) conjugated to epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF; or FGF-2) (3.9 and 6.7% w/w protein loading, respectively) to support the expansion and differentiation of stem cells in vitro via sustained, controllable growth factor release. Supplementation of mouse neural stem cells (mNSCs) with dextrin-growth factor conjugates led to greater and prolonged proliferation compared to unbound EGF/bFGF controls, with no detectable apoptosis after 7 days of treatment. Immunocytochemical detection of neural precursor (nestin) and differentiation (Olig2, MAP2, GFAP) markers verified that controlled release of dextrin-conjugated growth factors preserves stem cell properties of mNSCs for up to 7 days. These results show the potential of dextrin-growth factor conjugates for localized delivery of bioactive therapeutic agents to support stem cell expansion and differentiation, and as an adjunct to direct neuronal repair.
published_date 2018-12-01T04:20:34Z
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score 11.037056

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