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Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro

Kirsty Meldrum, Joana Amaral Duarte De Moura, Shareen Doak Orcid Logo, Martin Clift Orcid Logo

Nanomaterials, Volume: 12, Issue: 19, Start page: 3431

Swansea University Authors: Kirsty Meldrum, Joana Amaral Duarte De Moura, Shareen Doak Orcid Logo, Martin Clift Orcid Logo

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DOI (Published version): 10.3390/nano12193431

Abstract

The majority of in vitro studies focusing upon particle–lung cell interactions use static models at an air–liquid interface (ALI). Advancing the physiological characteristics of such systems allows for closer resemblance of the human lung, in turn promoting 3R strategies. PATROLS (EU Hori-zon 2020 N...

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Published in: Nanomaterials
ISSN: 2079-4991
Published: MDPI AG 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa61384
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Advancing the physiological characteristics of such systems allows for closer resemblance of the human lung, in turn promoting 3R strategies. PATROLS (EU Hori-zon 2020 No.760813) aimed to use a well-characterised in vitro model of the human alveolar ep-ithelial barrier to determine how fluid-flow dynamics would impact the outputs of the model following particle exposure. Using the QuasiVivoTM (Kirkstall Ltd., York, UK) system, fluid-flow conditions were applied to an A549 + dTHP-1 cell co-culture model cultured at the ALI. DQ12 and TiO2 (JRCNM01005a) were used as model particles to assess the in vitro systems’ sensitivity. Us-ing a quasi- and aerosol (VitroCell Cloud12, VitroCell Systems, Germany) exposure approach, cell cultures were exposed over 24hrs at IVIVE concentrations of 1 and 10 (DQ12) and 1.4 and 10.4 (TiO2) µg/cm2, respectively. We compared static and fluid flow conditions after both these expo-sure methods. The co-culture was subsequently assessed for its viability, membrane integrity and (pro-)inflammatory response (IL-8 and IL-6 production). The results suggested that the addition of fluid flow to this alveolar co-culture model can influence the viability, membrane integrity and inflammatory responses dependent on the particle type and exposure.</abstract><type>Journal Article</type><journal>Nanomaterials</journal><volume>12</volume><journalNumber>19</journalNumber><paginationStart>3431</paginationStart><paginationEnd/><publisher>MDPI AG</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2079-4991</issnElectronic><keywords>in vitro; micro-fluidics; fluid flow; co-culture; lung; nanoparticles; aerosol exposure; quasi-ALI exposure</keywords><publishedDay>30</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-09-30</publishedDate><doi>10.3390/nano12193431</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm>External research funder(s) paid the OA fee (includes OA grants disbursed by the Library)</apcterm><funders>This research was funded by the PATROLS project, European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No: 760813.</funders><projectreference/><lastEdited>2024-02-01T15:58:13.6155398</lastEdited><Created>2022-09-29T16:54:35.6007132</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Kirsty</firstname><surname>Meldrum</surname><order>1</order></author><author><firstname>Joana</firstname><surname>Amaral Duarte De Moura</surname><order>2</order></author><author><firstname>Shareen</firstname><surname>Doak</surname><orcid>0000-0002-6753-1987</orcid><order>3</order></author><author><firstname>Martin</firstname><surname>Clift</surname><orcid>0000-0001-6133-3368</orcid><order>4</order></author></authors><documents><document><filename>61384__25419__fcd68a942bd94fe8b6dc24775fadf45c.pdf</filename><originalFilename>61384_VoR.pdf</originalFilename><uploaded>2022-10-12T10:41:22.3288074</uploaded><type>Output</type><contentLength>1735552</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2022 by the authors. 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spelling v2 61384 2022-09-29 Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro bbb7bd27bfa3c6ffc73da8facfebc793 Kirsty Meldrum Kirsty Meldrum true false 8aaab6d4bf74b728fdd77352fc8a4a28 Joana Amaral Duarte De Moura Joana Amaral Duarte De Moura true false 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 2022-09-29 BMS The majority of in vitro studies focusing upon particle–lung cell interactions use static models at an air–liquid interface (ALI). Advancing the physiological characteristics of such systems allows for closer resemblance of the human lung, in turn promoting 3R strategies. PATROLS (EU Hori-zon 2020 No.760813) aimed to use a well-characterised in vitro model of the human alveolar ep-ithelial barrier to determine how fluid-flow dynamics would impact the outputs of the model following particle exposure. Using the QuasiVivoTM (Kirkstall Ltd., York, UK) system, fluid-flow conditions were applied to an A549 + dTHP-1 cell co-culture model cultured at the ALI. DQ12 and TiO2 (JRCNM01005a) were used as model particles to assess the in vitro systems’ sensitivity. Us-ing a quasi- and aerosol (VitroCell Cloud12, VitroCell Systems, Germany) exposure approach, cell cultures were exposed over 24hrs at IVIVE concentrations of 1 and 10 (DQ12) and 1.4 and 10.4 (TiO2) µg/cm2, respectively. We compared static and fluid flow conditions after both these expo-sure methods. The co-culture was subsequently assessed for its viability, membrane integrity and (pro-)inflammatory response (IL-8 and IL-6 production). The results suggested that the addition of fluid flow to this alveolar co-culture model can influence the viability, membrane integrity and inflammatory responses dependent on the particle type and exposure. Journal Article Nanomaterials 12 19 3431 MDPI AG 2079-4991 in vitro; micro-fluidics; fluid flow; co-culture; lung; nanoparticles; aerosol exposure; quasi-ALI exposure 30 9 2022 2022-09-30 10.3390/nano12193431 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) This research was funded by the PATROLS project, European Union’s Horizon 2020 Research and Innovation Programme under grant agreement No: 760813. 2024-02-01T15:58:13.6155398 2022-09-29T16:54:35.6007132 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Kirsty Meldrum 1 Joana Amaral Duarte De Moura 2 Shareen Doak 0000-0002-6753-1987 3 Martin Clift 0000-0001-6133-3368 4 61384__25419__fcd68a942bd94fe8b6dc24775fadf45c.pdf 61384_VoR.pdf 2022-10-12T10:41:22.3288074 Output 1735552 application/pdf Version of Record true © 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
spellingShingle Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
Kirsty Meldrum
Joana Amaral Duarte De Moura
Shareen Doak
Martin Clift
title_short Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
title_full Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
title_fullStr Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
title_full_unstemmed Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
title_sort Dynamic Fluid Flow Exacerbates the (Pro-)Inflammatory Effects of Aerosolised Engineered Nanomaterials In Vitro
author_id_str_mv bbb7bd27bfa3c6ffc73da8facfebc793
8aaab6d4bf74b728fdd77352fc8a4a28
8f70286908f67238a527a98cbf66d387
71bf49b157691e541950f5c3f49c9169
author_id_fullname_str_mv bbb7bd27bfa3c6ffc73da8facfebc793_***_Kirsty Meldrum
8aaab6d4bf74b728fdd77352fc8a4a28_***_Joana Amaral Duarte De Moura
8f70286908f67238a527a98cbf66d387_***_Shareen Doak
71bf49b157691e541950f5c3f49c9169_***_Martin Clift
author Kirsty Meldrum
Joana Amaral Duarte De Moura
Shareen Doak
Martin Clift
author2 Kirsty Meldrum
Joana Amaral Duarte De Moura
Shareen Doak
Martin Clift
format Journal article
container_title Nanomaterials
container_volume 12
container_issue 19
container_start_page 3431
publishDate 2022
institution Swansea University
issn 2079-4991
doi_str_mv 10.3390/nano12193431
publisher MDPI AG
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description The majority of in vitro studies focusing upon particle–lung cell interactions use static models at an air–liquid interface (ALI). Advancing the physiological characteristics of such systems allows for closer resemblance of the human lung, in turn promoting 3R strategies. PATROLS (EU Hori-zon 2020 No.760813) aimed to use a well-characterised in vitro model of the human alveolar ep-ithelial barrier to determine how fluid-flow dynamics would impact the outputs of the model following particle exposure. Using the QuasiVivoTM (Kirkstall Ltd., York, UK) system, fluid-flow conditions were applied to an A549 + dTHP-1 cell co-culture model cultured at the ALI. DQ12 and TiO2 (JRCNM01005a) were used as model particles to assess the in vitro systems’ sensitivity. Us-ing a quasi- and aerosol (VitroCell Cloud12, VitroCell Systems, Germany) exposure approach, cell cultures were exposed over 24hrs at IVIVE concentrations of 1 and 10 (DQ12) and 1.4 and 10.4 (TiO2) µg/cm2, respectively. We compared static and fluid flow conditions after both these expo-sure methods. The co-culture was subsequently assessed for its viability, membrane integrity and (pro-)inflammatory response (IL-8 and IL-6 production). The results suggested that the addition of fluid flow to this alveolar co-culture model can influence the viability, membrane integrity and inflammatory responses dependent on the particle type and exposure.
published_date 2022-09-30T15:58:14Z
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