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Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth

Alexander S. Tagg, Theodor Sperlea Orcid Logo, Matthias Labrenz, Jesse P. Harrison Orcid Logo, Jesus Ojeda Ledo Orcid Logo, Melanie Sapp Orcid Logo

Microorganisms, Volume: 10, Issue: 9, Start page: 1775

Swansea University Author: Jesus Ojeda Ledo Orcid Logo

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DOI (Published version): 10.3390/microorganisms10091775

Abstract

Microplastics are a globally-ubiquitous aquatic pollutant, and have been heavily studied over the last decade. Of particularly interest are the interactions between microplastics and microorgan-isms, especially the pursuit to discover a plastic-specific biome; the so called plastisphere. To follow t...

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Published in: Microorganisms
ISSN: 2076-2607
Published: MDPI AG 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa60987
first_indexed 2022-08-30T21:40:54Z
last_indexed 2023-01-13T19:21:31Z
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spelling 2022-09-13T15:07:39.2594716 v2 60987 2022-08-30 Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth 4c1c9800dffa623353dff0ab1271be64 0000-0002-2046-1010 Jesus Ojeda Ledo Jesus Ojeda Ledo true false 2022-08-30 EAAS Microplastics are a globally-ubiquitous aquatic pollutant, and have been heavily studied over the last decade. Of particularly interest are the interactions between microplastics and microorgan-isms, especially the pursuit to discover a plastic-specific biome; the so called plastisphere. To follow this up, a year-long microcosm experimental setup was deployed to expose 5 different microplastic types (and silica beads control) to activated aerobic wastewater in controlled condi-tions, with microbial communities being measured 4 times over the course of the year using 16S rDNA (bacterial) and ITS (fungal) amplicon sequencing. The biofilm community shows no evi-dence of a specific plastisphere, even after a year of incubation. Indeed, the microbial communities (particularly bacterial) show a clear trend of increasing dissimilarity between plastic types as time increases. Despite little evidence for a plastic-specific community, there was a slight grouping observed for polyolefins (PE and PP) in 6-12 month biofilms. Additionally, an OTU assigned to the genus Devosia was identified on many plastics, increasing over time, while showing no growth on silicate (natural particle) controls, suggesting this could be either a slow-growing plastic-specific taxon, or a symbiont to such. Both substrate-associated findings were only possible to observe in samples incubated for 6 - 12 months, which highlights the importance in studying long-term microbial community dynamics on plastic surfaces. Journal Article Microorganisms 10 9 1775 MDPI AG 2076-2607 2 9 2022 2022-09-02 10.3390/microorganisms10091775 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee This work was funded by a NERC (Natural Environment Research Council) CASE studentship (NE/K007521) with contribution from industrial partner Fera Science Ltd., United Kingdom. 2022-09-13T15:07:39.2594716 2022-08-30T22:27:44.0047079 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Alexander S. Tagg 1 Theodor Sperlea 0000-0003-4307-2963 2 Matthias Labrenz 3 Jesse P. Harrison 0000-0002-4877-4684 4 Jesus Ojeda Ledo 0000-0002-2046-1010 5 Melanie Sapp 0000-0002-0817-5425 6 60987__25070__50f8e09562c44d01a1e5d7cb733f83e0.pdf microorganisms-10-01775.pdf 2022-09-02T17:53:16.9500384 Output 1381730 application/pdf Version of Record true © 2022 by the authors. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license true eng https://cre-ativecommons.org/licenses/by/4.0/
title Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
spellingShingle Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
Jesus Ojeda Ledo
title_short Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
title_full Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
title_fullStr Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
title_full_unstemmed Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
title_sort Year-Long Microbial Succession on Microplastics in Wastewater: Chaotic Dynamics Outweigh Preferential Growth
author_id_str_mv 4c1c9800dffa623353dff0ab1271be64
author_id_fullname_str_mv 4c1c9800dffa623353dff0ab1271be64_***_Jesus Ojeda Ledo
author Jesus Ojeda Ledo
author2 Alexander S. Tagg
Theodor Sperlea
Matthias Labrenz
Jesse P. Harrison
Jesus Ojeda Ledo
Melanie Sapp
format Journal article
container_title Microorganisms
container_volume 10
container_issue 9
container_start_page 1775
publishDate 2022
institution Swansea University
issn 2076-2607
doi_str_mv 10.3390/microorganisms10091775
publisher MDPI AG
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
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description Microplastics are a globally-ubiquitous aquatic pollutant, and have been heavily studied over the last decade. Of particularly interest are the interactions between microplastics and microorgan-isms, especially the pursuit to discover a plastic-specific biome; the so called plastisphere. To follow this up, a year-long microcosm experimental setup was deployed to expose 5 different microplastic types (and silica beads control) to activated aerobic wastewater in controlled condi-tions, with microbial communities being measured 4 times over the course of the year using 16S rDNA (bacterial) and ITS (fungal) amplicon sequencing. The biofilm community shows no evi-dence of a specific plastisphere, even after a year of incubation. Indeed, the microbial communities (particularly bacterial) show a clear trend of increasing dissimilarity between plastic types as time increases. Despite little evidence for a plastic-specific community, there was a slight grouping observed for polyolefins (PE and PP) in 6-12 month biofilms. Additionally, an OTU assigned to the genus Devosia was identified on many plastics, increasing over time, while showing no growth on silicate (natural particle) controls, suggesting this could be either a slow-growing plastic-specific taxon, or a symbiont to such. Both substrate-associated findings were only possible to observe in samples incubated for 6 - 12 months, which highlights the importance in studying long-term microbial community dynamics on plastic surfaces.
published_date 2022-09-02T08:14:17Z
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