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Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction

Stephen Evans Orcid Logo, Rachel Lawrence, Martha Ilett, Michael J. Burgum Orcid Logo, Kirsty Meldrum, Nicole Hondow, Gareth Jenkins Orcid Logo, Martin Clift Orcid Logo, Shareen Doak Orcid Logo

Toxicology in Vitro, Volume: 83, Start page: 105415

Swansea University Authors: Stephen Evans Orcid Logo, Rachel Lawrence, Michael J. Burgum Orcid Logo, Kirsty Meldrum, Gareth Jenkins Orcid Logo, Martin Clift Orcid Logo, Shareen Doak Orcid Logo

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DOI (Published version): 10.1016/j.tiv.2022.105415

Abstract

Due to the expansive application of TiO2 and its variance in physico-chemical characteristics, the toxicological profile of TiO2, in all its various forms, requires evaluation. This study aimed to assess the hazard of five TiO2 particle-types in relation to their cytotoxic profile correlated to thei...

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Published in: Toxicology in Vitro
ISSN: 0887-2333
Published: Elsevier BV 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa60312
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spelling 2022-09-07T12:40:16.4026160 v2 60312 2022-06-23 Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction cfca981bdfb8492873a48cc1629def9a 0000-0002-5352-9800 Stephen Evans Stephen Evans true false 74fa942e1fcca16180802a2607c47bfb Rachel Lawrence Rachel Lawrence true false 9a3ab85e1f89e7664b83f1016d4a9a93 NULL Michael J. Burgum Michael J. Burgum true true bbb7bd27bfa3c6ffc73da8facfebc793 Kirsty Meldrum Kirsty Meldrum true false a44095d26187304e903da7ca778697b6 0000-0002-5437-8389 Gareth Jenkins Gareth Jenkins true false 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 2022-06-23 BMS Due to the expansive application of TiO2 and its variance in physico-chemical characteristics, the toxicological profile of TiO2, in all its various forms, requires evaluation. This study aimed to assess the hazard of five TiO2 particle-types in relation to their cytotoxic profile correlated to their cellular interaction, specifically in human lymphoblast (TK6) and type-II alveolar epithelial (A549) cells. Treatment with the test materials was undertaken at a concentration range of 1–100 μg/cm2 over 24 and 72 h exposure. TiO2 interaction with both cell types was visualised by transmission electron microscopy, supported by energy-dispersive X-ray. None of the TiO2 materials tested promoted cytotoxicity in either cell type over the concentration and time range studied. All materials were observed to interact with the A549 cells and were further noted to be internalised following 24 h exposure. In contrast, only the pigmentary rutile was internalised by TK6 lymphoblasts after 24 h exposure. Where uptake was observed there was no evidence, as determined by 2D microscopy techniques, of particle localisation within the nucleus of either cell type. This study indicates that industrially relevant TiO2 particles demonstrate cell interactions that are cell-type dependent and do not induce cytotoxicity at the applied dose range. Journal Article Toxicology in Vitro 83 105415 Elsevier BV 0887-2333 Titanium dioxide; Industrially relevant; Cytotoxicity; Cellular association; Cellular uptake 1 9 2022 2022-09-01 10.1016/j.tiv.2022.105415 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University SU Library paid the OA fee (TA Institutional Deal) This study was funded by the Titanium Dioxide Manufacturers Association (TDMA). 2022-09-07T12:40:16.4026160 2022-06-23T15:32:25.4590163 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Stephen Evans 0000-0002-5352-9800 1 Rachel Lawrence 2 Martha Ilett 3 Michael J. Burgum NULL 4 Kirsty Meldrum 5 Nicole Hondow 6 Gareth Jenkins 0000-0002-5437-8389 7 Martin Clift 0000-0001-6133-3368 8 Shareen Doak 0000-0002-6753-1987 9 60312__24485__e88f04dd356a4a57bebe5ff703ec4c7c.pdf 60312_VoR.pdf 2022-07-07T15:22:56.8668323 Output 1703113 application/pdf Version of Record true © 2022 The Author(s). This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/by/4.0/
title Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
spellingShingle Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
Stephen Evans
Rachel Lawrence
Michael J. Burgum
Kirsty Meldrum
Gareth Jenkins
Martin Clift
Shareen Doak
title_short Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
title_full Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
title_fullStr Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
title_full_unstemmed Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
title_sort Industrial-relevant TiO2 types do not promote cytotoxicity in the A549 or TK6 cell lines regardless of cell specific interaction
author_id_str_mv cfca981bdfb8492873a48cc1629def9a
74fa942e1fcca16180802a2607c47bfb
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bbb7bd27bfa3c6ffc73da8facfebc793
a44095d26187304e903da7ca778697b6
71bf49b157691e541950f5c3f49c9169
8f70286908f67238a527a98cbf66d387
author_id_fullname_str_mv cfca981bdfb8492873a48cc1629def9a_***_Stephen Evans
74fa942e1fcca16180802a2607c47bfb_***_Rachel Lawrence
9a3ab85e1f89e7664b83f1016d4a9a93_***_Michael J. Burgum
bbb7bd27bfa3c6ffc73da8facfebc793_***_Kirsty Meldrum
a44095d26187304e903da7ca778697b6_***_Gareth Jenkins
71bf49b157691e541950f5c3f49c9169_***_Martin Clift
8f70286908f67238a527a98cbf66d387_***_Shareen Doak
author Stephen Evans
Rachel Lawrence
Michael J. Burgum
Kirsty Meldrum
Gareth Jenkins
Martin Clift
Shareen Doak
author2 Stephen Evans
Rachel Lawrence
Martha Ilett
Michael J. Burgum
Kirsty Meldrum
Nicole Hondow
Gareth Jenkins
Martin Clift
Shareen Doak
format Journal article
container_title Toxicology in Vitro
container_volume 83
container_start_page 105415
publishDate 2022
institution Swansea University
issn 0887-2333
doi_str_mv 10.1016/j.tiv.2022.105415
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Due to the expansive application of TiO2 and its variance in physico-chemical characteristics, the toxicological profile of TiO2, in all its various forms, requires evaluation. This study aimed to assess the hazard of five TiO2 particle-types in relation to their cytotoxic profile correlated to their cellular interaction, specifically in human lymphoblast (TK6) and type-II alveolar epithelial (A549) cells. Treatment with the test materials was undertaken at a concentration range of 1–100 μg/cm2 over 24 and 72 h exposure. TiO2 interaction with both cell types was visualised by transmission electron microscopy, supported by energy-dispersive X-ray. None of the TiO2 materials tested promoted cytotoxicity in either cell type over the concentration and time range studied. All materials were observed to interact with the A549 cells and were further noted to be internalised following 24 h exposure. In contrast, only the pigmentary rutile was internalised by TK6 lymphoblasts after 24 h exposure. Where uptake was observed there was no evidence, as determined by 2D microscopy techniques, of particle localisation within the nucleus of either cell type. This study indicates that industrially relevant TiO2 particles demonstrate cell interactions that are cell-type dependent and do not induce cytotoxicity at the applied dose range.
published_date 2022-09-01T04:18:19Z
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