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ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it

Venkat Kanamarlapudi Orcid Logo, Salam Tamaddon Jahromi, Kate Murphy Orcid Logo

PLOS ONE, Volume: 17, Issue: 2, Start page: e0263845

Swansea University Authors: Venkat Kanamarlapudi Orcid Logo, Salam Tamaddon Jahromi

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DOI (Published version): 10.1371/journal.pone.0263845

Abstract

ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a w...

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Published in: PLOS ONE
ISSN: 1932-6203
Published: Public Library of Science (PLoS) 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa60111
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spelling 2022-06-14T11:08:38.1493487 v2 60111 2022-05-30 ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it 63741801137148abfa4c00cd547dcdfa 0000-0002-8739-1483 Venkat Kanamarlapudi Venkat Kanamarlapudi true false 1a74754e3fabec6379651cbd842c96bf Salam Tamaddon Jahromi Salam Tamaddon Jahromi true false 2022-05-30 BMS ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a wide range of tissues has not been studied so far. In this report, we analysed the expression of ARF6 mRNA in cancers and corresponding healthy controls from 17 different tissues by real-time qualitative polymerase chain reaction (RT-qPCR). We further evaluated ARF6 protein expression in oesophageal adenocarcinoma (EAC) tissue microarray cores by immunohistochemistry. The ARF6 gene expression levels are highly variable between healthy and cancer tissues. Our findings suggest that the ARF6 gene expression is up-regulated highest in oesophageal cancer. In EAC TMAs, ARF6 protein expression increase correlated with EAC progression. This is the first study to investigate ARF6 gene expression in a wide array of cancer tissues and demonstrate that ARF6 expression, at both mRNA and protein levels, is significantly upregulated in higher grades of EAC, which may be useful in targeting ARF6 for cancer diagnostic and therapeutic purposes. Journal Article PLOS ONE 17 2 e0263845 Public Library of Science (PLoS) 1932-6203 10 2 2022 2022-02-10 10.1371/journal.pone.0263845 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University VK: BBSRC UK (BB/F017596/1, BB/C515455/2 and BB/S019588/1) and MRC UK (G0401232) 2022-06-14T11:08:38.1493487 2022-05-30T18:01:55.5116818 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Venkat Kanamarlapudi 0000-0002-8739-1483 1 Salam Tamaddon Jahromi 2 Kate Murphy 0000-0003-2870-3018 3 60111__24304__2b541405918c450582bcca203962e5ab.pdf 60111.pdf 2022-06-14T11:06:41.7227799 Output 2203943 application/pdf Version of Record true © 2022 Kanamarlapudi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License true eng http://creativecommons.org/licenses/by/4.0/
title ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
spellingShingle ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
Venkat Kanamarlapudi
Salam Tamaddon Jahromi
title_short ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
title_full ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
title_fullStr ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
title_full_unstemmed ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
title_sort ADP-ribosylation factor 6 expression increase in oesophageal adenocarcinoma suggests a potential biomarker role for it
author_id_str_mv 63741801137148abfa4c00cd547dcdfa
1a74754e3fabec6379651cbd842c96bf
author_id_fullname_str_mv 63741801137148abfa4c00cd547dcdfa_***_Venkat Kanamarlapudi
1a74754e3fabec6379651cbd842c96bf_***_Salam Tamaddon Jahromi
author Venkat Kanamarlapudi
Salam Tamaddon Jahromi
author2 Venkat Kanamarlapudi
Salam Tamaddon Jahromi
Kate Murphy
format Journal article
container_title PLOS ONE
container_volume 17
container_issue 2
container_start_page e0263845
publishDate 2022
institution Swansea University
issn 1932-6203
doi_str_mv 10.1371/journal.pone.0263845
publisher Public Library of Science (PLoS)
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description ADP-ribosylation factor 6 small GTPase plays an important role in cell migration, invasion and angiogenesis, which are the hallmarks of cancer. Although alterations in ARF6 expression and activity have been linked to metastatic cancer in one or two tissues, the expression of ARF6 in cancers over a wide range of tissues has not been studied so far. In this report, we analysed the expression of ARF6 mRNA in cancers and corresponding healthy controls from 17 different tissues by real-time qualitative polymerase chain reaction (RT-qPCR). We further evaluated ARF6 protein expression in oesophageal adenocarcinoma (EAC) tissue microarray cores by immunohistochemistry. The ARF6 gene expression levels are highly variable between healthy and cancer tissues. Our findings suggest that the ARF6 gene expression is up-regulated highest in oesophageal cancer. In EAC TMAs, ARF6 protein expression increase correlated with EAC progression. This is the first study to investigate ARF6 gene expression in a wide array of cancer tissues and demonstrate that ARF6 expression, at both mRNA and protein levels, is significantly upregulated in higher grades of EAC, which may be useful in targeting ARF6 for cancer diagnostic and therapeutic purposes.
published_date 2022-02-10T04:17:57Z
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