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Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation

C. Pararasa, D.J. Messenger, K.E. Barrett, D. Hyliands, D. Talbot Orcid Logo, M.I. Fowler, T. Kawatra, D.A. Gunn, F.L. Lim Orcid Logo, L.J. Wainwright Orcid Logo, G. Jenkins, Helen Griffiths

Biochemical and Biophysical Research Communications, Volume: 601, Pages: 9 - 15

Swansea University Author: Helen Griffiths

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DOI (Published version): 10.1016/j.bbrc.2022.02.055

Abstract

Keratinocytes produce lipids that are critical for the skin barrier, however, little is known about the impact of age on fatty acid (FA) biosynthesis in these cells. We have examined the relationship between keratinocyte FA composition, lipid biosynthetic gene expression, gene promoter methylation a...

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Published in: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
Published: Elsevier BV 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa59412
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spelling 2022-09-07T12:09:19.9677593 v2 59412 2022-02-16 Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation 0366ea9a689b222146b7d63c6baf8427 Helen Griffiths Helen Griffiths true false 2022-02-16 SMT Keratinocytes produce lipids that are critical for the skin barrier, however, little is known about the impact of age on fatty acid (FA) biosynthesis in these cells. We have examined the relationship between keratinocyte FA composition, lipid biosynthetic gene expression, gene promoter methylation and age. Expression of elongase (ELOVL6 and 7) and desaturase (FADS1 and 2) genes was lower in adult versus neonatal keratinocytes, and was associated with lower concentrations of n-7, n-9 and n-10 polyunsaturated FA in adult cells. Consistent with these findings, transient FADS2 knockdown in neonatal keratinocytes mimicked the adult keratinocyte FA profile in neonatal cells. Interrogation of methylation levels across the FADS2 locus (53 genomic sites) revealed differential methylation of 15 sites in neonatal versus adult keratinocytes, of which three hypermethylated sites in adult keratinocytes overlapped with a SMARCA4 protein binding site in the FADS2 promoter. Journal Article Biochemical and Biophysical Research Communications 601 9 15 Elsevier BV 0006-291X Fatty acid; Epigenetic; mRNA; GC-MS; Keratinocytes; SMARCA4 23 4 2022 2022-04-23 10.1016/j.bbrc.2022.02.055 COLLEGE NANME Senior Leadership Team COLLEGE CODE SMT Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) BBSRC (BB/M005054/1) 2022-09-07T12:09:19.9677593 2022-02-16T11:54:56.3221915 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine C. Pararasa 1 D.J. Messenger 2 K.E. Barrett 3 D. Hyliands 4 D. Talbot 0000-0002-4503-4920 5 M.I. Fowler 6 T. Kawatra 7 D.A. Gunn 8 F.L. Lim 0000-0002-7744-4836 9 L.J. Wainwright 0000-0002-3372-9856 10 G. Jenkins 11 Helen Griffiths 12 59412__23719__1b435a060adf477f9d5de86c5c4320ea.pdf 59412.pdf 2022-03-29T12:29:45.0453682 Output 1273712 application/pdf Version of Record true © 2022 The Authors. This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/by/4.0/
title Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
spellingShingle Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
Helen Griffiths
title_short Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
title_full Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
title_fullStr Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
title_full_unstemmed Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
title_sort Lower polyunsaturated fatty acid levels and FADS2 expression in adult compared to neonatal keratinocytes are associated with FADS2 promotor hypermethylation
author_id_str_mv 0366ea9a689b222146b7d63c6baf8427
author_id_fullname_str_mv 0366ea9a689b222146b7d63c6baf8427_***_Helen Griffiths
author Helen Griffiths
author2 C. Pararasa
D.J. Messenger
K.E. Barrett
D. Hyliands
D. Talbot
M.I. Fowler
T. Kawatra
D.A. Gunn
F.L. Lim
L.J. Wainwright
G. Jenkins
Helen Griffiths
format Journal article
container_title Biochemical and Biophysical Research Communications
container_volume 601
container_start_page 9
publishDate 2022
institution Swansea University
issn 0006-291X
doi_str_mv 10.1016/j.bbrc.2022.02.055
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Keratinocytes produce lipids that are critical for the skin barrier, however, little is known about the impact of age on fatty acid (FA) biosynthesis in these cells. We have examined the relationship between keratinocyte FA composition, lipid biosynthetic gene expression, gene promoter methylation and age. Expression of elongase (ELOVL6 and 7) and desaturase (FADS1 and 2) genes was lower in adult versus neonatal keratinocytes, and was associated with lower concentrations of n-7, n-9 and n-10 polyunsaturated FA in adult cells. Consistent with these findings, transient FADS2 knockdown in neonatal keratinocytes mimicked the adult keratinocyte FA profile in neonatal cells. Interrogation of methylation levels across the FADS2 locus (53 genomic sites) revealed differential methylation of 15 sites in neonatal versus adult keratinocytes, of which three hypermethylated sites in adult keratinocytes overlapped with a SMARCA4 protein binding site in the FADS2 promoter.
published_date 2022-04-23T04:16:42Z
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