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Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture

John Thompson, Mark A. Weaver, Ingrid Lupatsch, Robin Shields, Sue Plummer, Christopher Coates, Andrew Rowley

Frontiers in Marine Science, Volume: 9

Swansea University Authors: Robin Shields, Christopher Coates, Andrew Rowley

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DOI (Published version): 10.3389/fmars.2022.807989

Abstract

Probiotic use in aquaculture settings can be an approach for disease control and dietary supplementation. We assessed the antagonistic effect of culture supernatants of lactic acid bacteria on the growth of known shrimp pathogens, Vibrio (Listonella) anguillarum, Vibrio alginolyticus, and V. harveyi...

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Published in: Frontiers in Marine Science
ISSN: 2296-7745
Published: Frontiers Media SA 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa59342
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We assessed the antagonistic effect of culture supernatants of lactic acid bacteria on the growth of known shrimp pathogens, Vibrio (Listonella) anguillarum, Vibrio alginolyticus, and V. harveyi, using a quantitative microplate bioassay. Supernatants from Lactobacillus curvatus subsp. curvatus, L. plantarum, and Pediococcus acidolactici significantly inhibited the growth of these vibrios. The active component(s) were heat stable (&gt; 100&#xB0;C) and resistant to freeze-thawing. Most of this inhibitory activity was brought about by the production of an acid pH; however, there was evidence for other factors playing a role. In the search for novel probiotic bacteria, an organism was isolated from the gastrointestinal tract of healthy whiteleg shrimp (Penaeus vannamei)&#x2014;identified tentatively as Carnobacterium maltaromaticum. This isolate, however, had less potent vibriocidal activity than the lactic acid bacteria and reduced shrimp survival at a dose of 1 &#xD7; 107 bacteria/shrimp. During a 28-day feeding trial, juvenile P. vannamei fed with L. plantarum supplemented diets showed no gross changes in growth parameters compared with the control. 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spelling 2022-08-05T11:38:00.2541345 v2 59342 2022-02-10 Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture 53cda6fb3899826e38f5bb4d69ba8c72 Robin Shields Robin Shields true false af160934b75bea5b8ba83d68b3d1a003 Christopher Coates Christopher Coates true false e98124f6e62b9592786899d7059e3a79 Andrew Rowley Andrew Rowley true false 2022-02-10 SBI Probiotic use in aquaculture settings can be an approach for disease control and dietary supplementation. We assessed the antagonistic effect of culture supernatants of lactic acid bacteria on the growth of known shrimp pathogens, Vibrio (Listonella) anguillarum, Vibrio alginolyticus, and V. harveyi, using a quantitative microplate bioassay. Supernatants from Lactobacillus curvatus subsp. curvatus, L. plantarum, and Pediococcus acidolactici significantly inhibited the growth of these vibrios. The active component(s) were heat stable (> 100°C) and resistant to freeze-thawing. Most of this inhibitory activity was brought about by the production of an acid pH; however, there was evidence for other factors playing a role. In the search for novel probiotic bacteria, an organism was isolated from the gastrointestinal tract of healthy whiteleg shrimp (Penaeus vannamei)—identified tentatively as Carnobacterium maltaromaticum. This isolate, however, had less potent vibriocidal activity than the lactic acid bacteria and reduced shrimp survival at a dose of 1 × 107 bacteria/shrimp. During a 28-day feeding trial, juvenile P. vannamei fed with L. plantarum supplemented diets showed no gross changes in growth parameters compared with the control. We suggest that lactic acid bacteria could be incorporated into biofloc formulations to purge the growth of pathogenic vibrios in pond settings, rather than being fed directly to shrimp. Journal Article Frontiers in Marine Science 9 Frontiers Media SA 2296-7745 aquaculture, shellfish health, disease, biofloc, competitive exclusion, Carnobacterium maltaromaticum, Vibrio harveyi, Vibrio alginolyticus 28 2 2022 2022-02-28 10.3389/fmars.2022.807989 COLLEGE NANME Biosciences COLLEGE CODE SBI Swansea University JT was supported by the European Social Fund doctoral training grant. AR and CC were supported by the BBSRC/NERC ARCHUK network grant (BB/P017215/1). 2022-08-05T11:38:00.2541345 2022-02-10T11:11:39.4421086 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences John Thompson 1 Mark A. Weaver 2 Ingrid Lupatsch 3 Robin Shields 4 Sue Plummer 5 Christopher Coates 6 Andrew Rowley 7 59342__23773__fe2c9db4911b4e2b8dde4c31ad63213e.pdf 59342.pdf 2022-04-04T16:58:29.5047896 Output 1296671 application/pdf Version of Record true Copyright © 2022 Thompson, Weaver, Lupatsch, Shields, Plummer, Coates and Rowley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). true eng http://creativecommons.org/licenses/by/4.0/
title Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
spellingShingle Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
Robin Shields
Christopher Coates
Andrew Rowley
title_short Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
title_full Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
title_fullStr Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
title_full_unstemmed Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
title_sort Antagonistic Activity of Lactic Acid Bacteria Against Pathogenic Vibrios and Their Potential Use as Probiotics in Shrimp (Penaeus vannamei) Culture
author_id_str_mv 53cda6fb3899826e38f5bb4d69ba8c72
af160934b75bea5b8ba83d68b3d1a003
e98124f6e62b9592786899d7059e3a79
author_id_fullname_str_mv 53cda6fb3899826e38f5bb4d69ba8c72_***_Robin Shields
af160934b75bea5b8ba83d68b3d1a003_***_Christopher Coates
e98124f6e62b9592786899d7059e3a79_***_Andrew Rowley
author Robin Shields
Christopher Coates
Andrew Rowley
author2 John Thompson
Mark A. Weaver
Ingrid Lupatsch
Robin Shields
Sue Plummer
Christopher Coates
Andrew Rowley
format Journal article
container_title Frontiers in Marine Science
container_volume 9
publishDate 2022
institution Swansea University
issn 2296-7745
doi_str_mv 10.3389/fmars.2022.807989
publisher Frontiers Media SA
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description Probiotic use in aquaculture settings can be an approach for disease control and dietary supplementation. We assessed the antagonistic effect of culture supernatants of lactic acid bacteria on the growth of known shrimp pathogens, Vibrio (Listonella) anguillarum, Vibrio alginolyticus, and V. harveyi, using a quantitative microplate bioassay. Supernatants from Lactobacillus curvatus subsp. curvatus, L. plantarum, and Pediococcus acidolactici significantly inhibited the growth of these vibrios. The active component(s) were heat stable (> 100°C) and resistant to freeze-thawing. Most of this inhibitory activity was brought about by the production of an acid pH; however, there was evidence for other factors playing a role. In the search for novel probiotic bacteria, an organism was isolated from the gastrointestinal tract of healthy whiteleg shrimp (Penaeus vannamei)—identified tentatively as Carnobacterium maltaromaticum. This isolate, however, had less potent vibriocidal activity than the lactic acid bacteria and reduced shrimp survival at a dose of 1 × 107 bacteria/shrimp. During a 28-day feeding trial, juvenile P. vannamei fed with L. plantarum supplemented diets showed no gross changes in growth parameters compared with the control. We suggest that lactic acid bacteria could be incorporated into biofloc formulations to purge the growth of pathogenic vibrios in pond settings, rather than being fed directly to shrimp.
published_date 2022-02-28T04:16:35Z
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