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Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>

Samantha Llewellyn, Wolfgang J. Parak, Jonas Hühn, Michael Burgum, Stephen Evans Orcid Logo, Katherine Chapman Orcid Logo, Gareth Jenkins Orcid Logo, Shareen Doak Orcid Logo, Martin Clift Orcid Logo

Nanotoxicology, Volume: 16, Issue: 1, Pages: 52 - 72

Swansea University Authors: Samantha Llewellyn, Michael Burgum, Stephen Evans Orcid Logo, Katherine Chapman Orcid Logo, Gareth Jenkins Orcid Logo, Shareen Doak Orcid Logo, Martin Clift Orcid Logo

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DOI (Published version): 10.1080/17435390.2022.2030823

Abstract

Human ENP exposure is inevitable and the novel, size dependent physico-chemical properties that enable ENPs to be beneficial in innovative technologies are concomitantly causing heightened public concerns as to their potential adverse effects upon human health. This study aims to deduce the mechanis...

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Published in: Nanotoxicology
ISSN: 1743-5390 1743-5404
Published: Informa UK Limited 2022
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URI: https://cronfa.swan.ac.uk/Record/cronfa59210
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spelling 2022-10-26T14:58:31.4719028 v2 59210 2022-01-18 Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i> fb4b6c686b654c6687c4063974b5cc8c Samantha Llewellyn Samantha Llewellyn true false d3fe156a5ee169e586b8bad6ae4cb1d8 Michael Burgum Michael Burgum true false cfca981bdfb8492873a48cc1629def9a 0000-0002-5352-9800 Stephen Evans Stephen Evans true false 19e7d85eec17117858d867ec0c9f575e 0000-0001-6668-0705 Katherine Chapman Katherine Chapman true false a44095d26187304e903da7ca778697b6 0000-0002-5437-8389 Gareth Jenkins Gareth Jenkins true false 8f70286908f67238a527a98cbf66d387 0000-0002-6753-1987 Shareen Doak Shareen Doak true false 71bf49b157691e541950f5c3f49c9169 0000-0001-6133-3368 Martin Clift Martin Clift true false 2022-01-18 BMS Human ENP exposure is inevitable and the novel, size dependent physico-chemical properties that enable ENPs to be beneficial in innovative technologies are concomitantly causing heightened public concerns as to their potential adverse effects upon human health. This study aims to deduce the mechanisms associated with potential ENP mediated (geno)toxicity and impact upon telomere integrity, if any, of varying concentrations of both ~16 nm (4.34x10-3 - 17.36x10-3 mg/mL) Gold (Au) and ~14 nm (0.85x10-5 - 3.32x10-5 mg/mL) Silver (Ag) ENPs upon two commonly used lung epithelial cell lines, 16HBE14o- and A549. Following cytotoxicity analysis (via Trypan Blue and Lactate Dehydrogenase assay), two sub-lethal concentrations were selected for genotoxicity analysis using the cytokinesis-blocked micronucleus assay. Whilst both ENP types induced significant oxidative stress, Ag ENPs (1.66x10-5 mg/mL) did not display a significant genotoxic response in either epithelial cell lines, but Au ENPs (8.68x10-3 mg/mL) showed a highly significant 2.63-fold and 2.4-fold increase in micronucleus frequency in A549 and 16HBE14o- cells respectively. It is hypothesised that the DNA damage induced by acute 24-hour Au ENP exposure resulted in a cell cycle stall indicated by the increased mononuclear cell fraction (>6.0-fold) and cytostasis level. Albeit insignificant, a small reduction in telomere length was observed following acute exposure to both ENPs which could indicate potential for ENP mediated telomere attrition. Finally, from the data shown, both in vitro lung cell cultures (16HBE14o- and A549) are equally as suitable and reliable for the in vitro ENP hazard identification approach adopted in this study. Journal Article Nanotoxicology 16 1 52 72 Informa UK Limited 1743-5390 1743-5404 Gold; silver; engineered nanoparticles; in vitro genotoxicity; lung epithelial cell lines 27 1 2022 2022-01-27 10.1080/17435390.2022.2030823 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University SU Library paid the OA fee (TA Institutional Deal) 2022-10-26T14:58:31.4719028 2022-01-18T12:47:19.8092037 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Samantha Llewellyn 1 Wolfgang J. Parak 2 Jonas Hühn 3 Michael Burgum 4 Stephen Evans 0000-0002-5352-9800 5 Katherine Chapman 0000-0001-6668-0705 6 Gareth Jenkins 0000-0002-5437-8389 7 Shareen Doak 0000-0002-6753-1987 8 Martin Clift 0000-0001-6133-3368 9 59210__24060__c325fac6aac649a98b7d4453b77ca45d.pdf 59210_VOR.pdf 2022-05-13T09:46:14.8980123 Output 4809433 application/pdf Version of Record true © 2022 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License true eng http://creativecommons.org/licenses/by-nc-nd/4.0/
title Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
spellingShingle Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
Samantha Llewellyn
Michael Burgum
Stephen Evans
Katherine Chapman
Gareth Jenkins
Shareen Doak
Martin Clift
title_short Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
title_full Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
title_fullStr Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
title_full_unstemmed Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
title_sort Deducing the cellular mechanisms associated with the potential genotoxic impact of gold and silver engineered nanoparticles upon different lung epithelial cell lines <i>in vitro</i>
author_id_str_mv fb4b6c686b654c6687c4063974b5cc8c
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author_id_fullname_str_mv fb4b6c686b654c6687c4063974b5cc8c_***_Samantha Llewellyn
d3fe156a5ee169e586b8bad6ae4cb1d8_***_Michael Burgum
cfca981bdfb8492873a48cc1629def9a_***_Stephen Evans
19e7d85eec17117858d867ec0c9f575e_***_Katherine Chapman
a44095d26187304e903da7ca778697b6_***_Gareth Jenkins
8f70286908f67238a527a98cbf66d387_***_Shareen Doak
71bf49b157691e541950f5c3f49c9169_***_Martin Clift
author Samantha Llewellyn
Michael Burgum
Stephen Evans
Katherine Chapman
Gareth Jenkins
Shareen Doak
Martin Clift
author2 Samantha Llewellyn
Wolfgang J. Parak
Jonas Hühn
Michael Burgum
Stephen Evans
Katherine Chapman
Gareth Jenkins
Shareen Doak
Martin Clift
format Journal article
container_title Nanotoxicology
container_volume 16
container_issue 1
container_start_page 52
publishDate 2022
institution Swansea University
issn 1743-5390
1743-5404
doi_str_mv 10.1080/17435390.2022.2030823
publisher Informa UK Limited
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Human ENP exposure is inevitable and the novel, size dependent physico-chemical properties that enable ENPs to be beneficial in innovative technologies are concomitantly causing heightened public concerns as to their potential adverse effects upon human health. This study aims to deduce the mechanisms associated with potential ENP mediated (geno)toxicity and impact upon telomere integrity, if any, of varying concentrations of both ~16 nm (4.34x10-3 - 17.36x10-3 mg/mL) Gold (Au) and ~14 nm (0.85x10-5 - 3.32x10-5 mg/mL) Silver (Ag) ENPs upon two commonly used lung epithelial cell lines, 16HBE14o- and A549. Following cytotoxicity analysis (via Trypan Blue and Lactate Dehydrogenase assay), two sub-lethal concentrations were selected for genotoxicity analysis using the cytokinesis-blocked micronucleus assay. Whilst both ENP types induced significant oxidative stress, Ag ENPs (1.66x10-5 mg/mL) did not display a significant genotoxic response in either epithelial cell lines, but Au ENPs (8.68x10-3 mg/mL) showed a highly significant 2.63-fold and 2.4-fold increase in micronucleus frequency in A549 and 16HBE14o- cells respectively. It is hypothesised that the DNA damage induced by acute 24-hour Au ENP exposure resulted in a cell cycle stall indicated by the increased mononuclear cell fraction (>6.0-fold) and cytostasis level. Albeit insignificant, a small reduction in telomere length was observed following acute exposure to both ENPs which could indicate potential for ENP mediated telomere attrition. Finally, from the data shown, both in vitro lung cell cultures (16HBE14o- and A549) are equally as suitable and reliable for the in vitro ENP hazard identification approach adopted in this study.
published_date 2022-01-27T04:16:20Z
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