Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Tizaoui, Chedly; Stanton, Richard; Statkute, Evelina; Rubina, Anzelika; Lester-Card, Edward; Lewis, Anthony; Holliman, Peter; Worsley, David

doi:10.1016/j.jhazmat.2022.128251

Journal article 640 views 167 downloads

Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Chedly Tizaoui

, Richard Stanton, Evelina Statkute, Anzelika Rubina, Edward Lester-Card, Anthony Lewis, Peter Holliman

, David Worsley

Journal of Hazardous Materials, Volume: 428, Start page: 128251

Swansea University Authors: Chedly Tizaoui , Edward Lester-Card, Anthony Lewis, Peter Holliman , David Worsley

PDF | Version of Record

© 2022 The Authors. This is an open access article under the CC BY license
Download (2.13MB)

Check full text

DOI (Published version): 10.1016/j.jhazmat.2022.128251

Abstract

This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SA...

Full description

Published in:	Journal of Hazardous Materials
ISSN:	0304-3894
Published:	Elsevier BV 2022
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa59183
Tags:	Add Tag No Tags, Be the first to tag this record!

first_indexed	2022-01-14T19:13:13Z
last_indexed	2023-01-11T14:40:17Z
id	cronfa59183
recordtype	SURis
fullrecord	<?xml version="1.0" encoding="utf-8"?><rfc1807 xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xsd="http://www.w3.org/2001/XMLSchema"><bib-version>v2</bib-version><id>59183</id><entry>2022-01-14</entry><title>Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media</title><swanseaauthors><author><sid>4b34a0286d3c0b0b081518fa6987031d</sid><ORCID>0000-0003-2159-7881</ORCID><firstname>Chedly</firstname><surname>Tizaoui</surname><name>Chedly Tizaoui</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>021c5bbf5c153fa16bcbbb8514e35869</sid><firstname>Edward</firstname><surname>Lester-Card</surname><name>Edward Lester-Card</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>328c21711ee3091505363e2b5060fba0</sid><firstname>Anthony</firstname><surname>Lewis</surname><name>Anthony Lewis</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c8f52394d776279c9c690dc26066ddf9</sid><ORCID>0000-0002-9911-8513</ORCID><firstname>Peter</firstname><surname>Holliman</surname><name>Peter Holliman</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>c426b1c1b0123d7057c1b969083cea69</sid><ORCID>0000-0002-9956-6228</ORCID><firstname>David</firstname><surname>Worsley</surname><name>David Worsley</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2022-01-14</date><deptcode>CHEG</deptcode><abstract>This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19.</abstract><type>Journal Article</type><journal>Journal of Hazardous Materials</journal><volume>428</volume><journalNumber/><paginationStart>128251</paginationStart><paginationEnd/><publisher>Elsevier BV</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint>0304-3894</issnPrint><issnElectronic/><keywords>SARS-CoV-2; Ozone; Disinfection; Sanitisation; Virus</keywords><publishedDay>15</publishedDay><publishedMonth>4</publishedMonth><publishedYear>2022</publishedYear><publishedDate>2022-04-15</publishedDate><doi>10.1016/j.jhazmat.2022.128251</doi><url/><notes/><college>COLLEGE NANME</college><department>Chemical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>CHEG</DepartmentCode><institution>Swansea University</institution><apcterm>SU Library paid the OA fee (TA Institutional Deal)</apcterm><funders>This work was supported by the Welsh Government Small Business Research Initiative (SBRI) Centre of Excellence - Welsh Ambulance Service & Defence and Security Accelerator, United Kingdom (ACC2014616).</funders><projectreference/><lastEdited>2023-04-28T15:58:23.6054495</lastEdited><Created>2022-01-14T19:07:53.6409170</Created><path><level id="1">Faculty of Science and Engineering</level><level id="2">School of Engineering and Applied Sciences - Materials Science and Engineering</level></path><authors><author><firstname>Chedly</firstname><surname>Tizaoui</surname><orcid>0000-0003-2159-7881</orcid><order>1</order></author><author><firstname>Richard</firstname><surname>Stanton</surname><order>2</order></author><author><firstname>Evelina</firstname><surname>Statkute</surname><order>3</order></author><author><firstname>Anzelika</firstname><surname>Rubina</surname><order>4</order></author><author><firstname>Edward</firstname><surname>Lester-Card</surname><order>5</order></author><author><firstname>Anthony</firstname><surname>Lewis</surname><order>6</order></author><author><firstname>Peter</firstname><surname>Holliman</surname><orcid>0000-0002-9911-8513</orcid><order>7</order></author><author><firstname>David</firstname><surname>Worsley</surname><orcid>0000-0002-9956-6228</orcid><order>8</order></author></authors><documents><document><filename>59183__22305__a2f17dffd280430a9816f922c892b102.pdf</filename><originalFilename>59183.pdf</originalFilename><uploaded>2022-02-04T14:42:01.4572672</uploaded><type>Output</type><contentLength>2235941</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>© 2022 The Authors. This is an open access article under the CC BY license</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling	v2 59183 2022-01-14 Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media 4b34a0286d3c0b0b081518fa6987031d 0000-0003-2159-7881 Chedly Tizaoui Chedly Tizaoui true false 021c5bbf5c153fa16bcbbb8514e35869 Edward Lester-Card Edward Lester-Card true false 328c21711ee3091505363e2b5060fba0 Anthony Lewis Anthony Lewis true false c8f52394d776279c9c690dc26066ddf9 0000-0002-9911-8513 Peter Holliman Peter Holliman true false c426b1c1b0123d7057c1b969083cea69 0000-0002-9956-6228 David Worsley David Worsley true false 2022-01-14 CHEG This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19. Journal Article Journal of Hazardous Materials 428 128251 Elsevier BV 0304-3894 SARS-CoV-2; Ozone; Disinfection; Sanitisation; Virus 15 4 2022 2022-04-15 10.1016/j.jhazmat.2022.128251 COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University SU Library paid the OA fee (TA Institutional Deal) This work was supported by the Welsh Government Small Business Research Initiative (SBRI) Centre of Excellence - Welsh Ambulance Service & Defence and Security Accelerator, United Kingdom (ACC2014616). 2023-04-28T15:58:23.6054495 2022-01-14T19:07:53.6409170 Faculty of Science and Engineering School of Engineering and Applied Sciences - Materials Science and Engineering Chedly Tizaoui 0000-0003-2159-7881 1 Richard Stanton 2 Evelina Statkute 3 Anzelika Rubina 4 Edward Lester-Card 5 Anthony Lewis 6 Peter Holliman 0000-0002-9911-8513 7 David Worsley 0000-0002-9956-6228 8 59183__22305__a2f17dffd280430a9816f922c892b102.pdf 59183.pdf 2022-02-04T14:42:01.4572672 Output 2235941 application/pdf Version of Record true © 2022 The Authors. This is an open access article under the CC BY license true eng http://creativecommons.org/licenses/by/4.0/
title	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
spellingShingle	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media Chedly Tizaoui Edward Lester-Card Anthony Lewis Peter Holliman David Worsley
title_short	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_full	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_fullStr	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_full_unstemmed	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
title_sort	Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media
author_id_str_mv	4b34a0286d3c0b0b081518fa6987031d 021c5bbf5c153fa16bcbbb8514e35869 328c21711ee3091505363e2b5060fba0 c8f52394d776279c9c690dc26066ddf9 c426b1c1b0123d7057c1b969083cea69
author_id_fullname_str_mv	4b34a0286d3c0b0b081518fa6987031d_*_Chedly Tizaoui 021c5bbf5c153fa16bcbbb8514e35869__Edward Lester-Card 328c21711ee3091505363e2b5060fba0__Anthony Lewis c8f52394d776279c9c690dc26066ddf9__Peter Holliman c426b1c1b0123d7057c1b969083cea69_**_David Worsley
author	Chedly Tizaoui Edward Lester-Card Anthony Lewis Peter Holliman David Worsley
author2	Chedly Tizaoui Richard Stanton Evelina Statkute Anzelika Rubina Edward Lester-Card Anthony Lewis Peter Holliman David Worsley
format	Journal article
container_title	Journal of Hazardous Materials
container_volume	428
container_start_page	128251
publishDate	2022
institution	Swansea University
issn	0304-3894
doi_str_mv	10.1016/j.jhazmat.2022.128251
publisher	Elsevier BV
college_str	Faculty of Science and Engineering
hierarchytype
hierarchy_top_id	facultyofscienceandengineering
hierarchy_top_title	Faculty of Science and Engineering
hierarchy_parent_id	facultyofscienceandengineering
hierarchy_parent_title	Faculty of Science and Engineering
department_str	School of Engineering and Applied Sciences - Materials Science and Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Materials Science and Engineering
document_store_str	1
active_str	0
description	This study evaluated the inactivation of SARS-CoV-2, the virus responsible for COVID-19, by ozone using virus grown in cell culture media either dried on surfaces (plastic, glass, stainless steel, copper, and coupons of ambulance seat and floor) or suspended in liquid. Treatment in liquid reduced SARS-CoV-2 at a rate of 0.92 ± 0.11 log10-reduction per ozone CT dose(mg min/L); where CT is ozone concentration times exposure time. On surface, the synergistic effect of CT and relative humidity (RH) was key to virus inactivation; the rate varied from 0.01 to 0.27 log10-reduction per ozone CT value(g min/m3) as RH varied from 17% to 70%. Depletion of ozone by competitive reactions with the medium constituents, mass transfer limiting the penetration of ozone to the bulk of the medium, and occlusion of the virus in dried matrix were postulated as potential mechanisms that reduce ozone efficacy. RH70% was found plausible since it provided the highest disinfection rate while being below the critical RH that promotes mould growth in buildings. In conclusion, through careful choice of (CT, RH), gaseous ozone is effective against SARS-CoV-2 and our results are of significance to a growing field where ozone is applied to control the spread of COVID-19.
published_date	2022-04-15T15:58:21Z
_version_	1764432440760205312
score	11.013194

Ozone for SARS-CoV-2 inactivation on surfaces and in liquid cell culture media

Similar Items